ABSTRACT
PURPOSE: In this study, the antiproliferative effects of the aromatase inhibitor anastrozole (arimidex®) was evaluated on estrogen receptor (ER) positive FM3A cell line originated from C3H mouse mammary carcinoma. METHODS: For this purpose cell kinetic parameters including viability analysis, mitotic index and labelling index were used. Three different doses of anostrozole (D1= 0.01 µM, D2= 0.1µM, D3= 1µM) were applied to cells for 24 h to determine the most effective dose. A dose of 1µM dose was determined as the most effective and this was used in all subsequent applications for 0-72 h. RESULTS: The results showed that there was a significant decrease in viability, mitotic index and labelling index for all experimental groups. The differences between control and all experimental groups were statistically significant (p<0.01) for all applications. CONCLUSIONS: Anastrozole offers a promising treatment modality in estrogen sensitive breast cancer.
Subject(s)
Antineoplastic Agents, Hormonal/pharmacology , Aromatase Inhibitors/pharmacology , Breast Neoplasms/pathology , Cell Proliferation/drug effects , Neoplasms, Hormone-Dependent/pathology , Nitriles/pharmacology , Triazoles/pharmacology , Anastrozole , Animals , Breast Neoplasms/metabolism , Cell Line, Tumor , Cell Survival/drug effects , Dose-Response Relationship, Drug , Female , Mice , Mice, Inbred C3H , Mitotic Index , Neoplasms, Hormone-Dependent/metabolism , Receptors, Estrogen/metabolism , Time FactorsABSTRACT
PURPOSE: In this study, the antiproliferative and apoptotic effects of sunitinib (SU-11248, Sutent) which is used for targeted therapy was evaluated on HeLa cell line originated from human cervix carcinoma. METHODS: Three different doses of sutent (D1= 1 µM, D2= 5 µM, D3= 10 µM) were administered to cells for 72 h to determine the optimal dose. RESULTS: Increase in apoptotic index (AI), decrease in mitotic index (MI) of cells and slow down in proliferation rate were achieved at the dose level of 10 µM, especially at 72 h. All these findings were statistically significant (p<0.001). In addition, anaphase bridges and existence of tripolar metaphase cells that were observed at 72 h were possibly attributable to a chromosomal instability arising from shortening of telomere. CONCLUSION: In this study, sutent effected cell kinetic parameters significantly. These results are consistent with other studies in the literature. In addition, anaphase bridges which were seen in mitosis preparations were interpreted as shortening or degradation of the telomere.
Subject(s)
Angiogenesis Inhibitors/pharmacology , Cell Proliferation/drug effects , Indoles/pharmacology , Protein Kinase Inhibitors/pharmacology , Pyrroles/pharmacology , Receptor Protein-Tyrosine Kinases/antagonists & inhibitors , Uterine Cervical Neoplasms/enzymology , Apoptosis/drug effects , Dose-Response Relationship, Drug , Female , HeLa Cells , Humans , Mitotic Index , Molecular Targeted Therapy , Oxidoreductases/metabolism , Receptor Protein-Tyrosine Kinases/metabolism , Sunitinib , Tetrazolium Salts/metabolism , Time Factors , Uterine Cervical Neoplasms/pathologyABSTRACT
Cancer stem cells (CSCs) possess several characteristics including self-renewal, pluripotency and tumorigenicity and constitute a rare population in a tumor mass. Because conventional cancer therapies can not kill CSCs, these cells are responsible for tumor relapse and metastasis. Currently, with advances in the identification of CSCs, the importance of these cells is increasing in the field of cancer diagnosis and prognosis. In addition, clarifying the mechanisms responsible for the maintenance of CSCs properties led to the development of CSC-targeted therapies.
Subject(s)
Neoplasms/drug therapy , Neoplastic Stem Cells/drug effects , Animals , Drug Resistance, Neoplasm , Humans , Neoplasms/diagnosis , PrognosisABSTRACT
The effect of epirubicin (EPI) on 3H-thymidine labelling index (3H-TdR LI) of L-strain cells in culture was investigated. EPI concentrations of 0.001 microg/ml, 0.01 microg/ml and 0.1 microg/ml were applied to the cells for 4, 8, 16 and 32 hours. Following the treatment with EPI, labelling process in 3H-TdR medium continued for 0, 2, 4, 8, 16, 20, 22, 25 and 30 hours. The results showed that EPI diminished labelling index of L-strain cells depends on time and applied concentrations. When compared to control this decrease was found statistically significant (p<0.001) in each group.
