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1.
Plant Cell Environ ; 2024 Jul 15.
Article in English | MEDLINE | ID: mdl-39007522

ABSTRACT

Living organisms have the capacity to respond to environmental stimuli, including warm conditions. Upon sensing mild temperature, plants launch a transcriptional response that promotes morphological changes, globally known as thermomorphogenesis. This response is orchestrated by different hormonal networks and by the activity of different transcription factors, including the heat shock factor A1 (HSFA1) family. Members of this family interact with heat shock protein 70 (HSP70) and heat shock protein 90 (HSP90); however, the effect of this binding on the regulation of HSFA1 activity or of the role of cochaperones, such as the HSP70-HSP90 organizing protein (HOP) on HSFA1 regulation, remains unknown. Here, we show that AtHOPs are involved in the folding and stabilization of the HSFA1a and are required for the onset of the transcriptional response associated to thermomorphogenesis. Our results demonstrate that the three members of the AtHOP family bind in vivo to the HSFA1a and that the expression of multiple HSFA1a-responsive-responsive genes is altered in the hop1 hop2 hop3 mutant under warm temperature. Interestingly, HSFA1a is accumulated at lower levels in the hop1 hop2 hop3 mutant, while control levels are recovered in the presence of the proteasome inhibitor MG132 or the synthetic chaperone tauroursodeoxycholic acid (TUDCA). This uncovers the HSFA1a as a client of HOP complexes in plants and reveals the participation of HOPs in HSFA1a stability.

2.
Viruses ; 16(2)2024 01 30.
Article in English | MEDLINE | ID: mdl-38399981

ABSTRACT

Alphaviruses can replicate in arthropods and in many vertebrate species including humankind, but only in vertebrate cells do infections with these viruses result in a strong inhibition of host translation and transcription. Translation shutoff by alphaviruses is a multifactorial process that involves both host- and virus-induced mechanisms, and some of them are not completely understood. Alphavirus genomes contain cis-acting elements (RNA structures and dinucleotide composition) and encode protein activities that promote the translational and transcriptional resistance to type I IFN-induced antiviral effectors. Among them, IFIT1, ZAP and PKR have played a relevant role in alphavirus evolution, since they have promoted the emergence of multiple viral evasion mechanisms at the translational level. In this review, we will discuss how the adaptations of alphaviruses to vertebrate hosts likely involved the acquisition of new features in viral mRNAs and proteins to overcome the effect of type I IFN.


Subject(s)
Alphavirus , Interferon Type I , Animals , Alphavirus/physiology , Cell Line , Interferon Type I/genetics , Vertebrates , Tropism , Antiviral Agents/pharmacology , Virus Replication
3.
J Exp Bot ; 75(14): 4274-4286, 2024 Jul 23.
Article in English | MEDLINE | ID: mdl-38330220

ABSTRACT

Proteins need to acquire their native structure in order to become fully functional. In specific cases, the active conformation is obtained spontaneously; nevertheless, many proteins need the assistance of chaperones and co-chaperones to be properly folded. These proteins help to maintain protein homeostasis under control conditions and under different stresses. HOP (HSP70-HSP90 organizing protein) is a highly conserved family of co-chaperones that assist HSP70 and HSP90 in the folding of specific proteins. In the last few years, findings in mammals and yeast have revealed novel functions of HOP and re-defined the role of HOP in protein folding. Here, we provide an overview of the most important aspects of HOP regulation and function in other eukaryotes and analyse whether these aspects are conserved in plants. In addition, we highlight the HOP clients described in plants and the role of HOP in plant development and stress response.


Subject(s)
Homeostasis , Plant Proteins , Stress, Physiological , Plant Proteins/metabolism , Plant Proteins/genetics , Molecular Chaperones/metabolism , Plant Development , Plants/metabolism
4.
Plant Cell Environ ; 45(8): 2508-2519, 2022 08.
Article in English | MEDLINE | ID: mdl-35610185

ABSTRACT

HOP (HSP70-HSP90 organising protein) is a conserved family of co-chaperones well known in mammals for its role in the folding of signalling proteins associated with development. In plants, HOP proteins have been involved in the response to multiple stresses, but their role in plant development remains elusive. Herein, we describe that the members of the HOP family participate in different aspects of plant development as well as in the response to warm temperatures through the regulation of auxin signalling. Arabidopsis hop1 hop2 hop3 triple mutant shows different auxin-related phenotypes and a reduced auxin sensitivity. HOP interacts with TIR1 auxin coreceptor in vivo. Furthermore, TIR1 accumulation and auxin transcriptional response are reduced in the hop1 hop2 hop3 triple mutant, suggesting that HOP's function in auxin signalling is related, at least, to TIR1 interaction and stabilisation. Interestingly, HOP proteins form part of the same complexes as SGT1b (a different HSP90 co-chaperone) and these co-chaperones synergistically cooperate in auxin signalling. This study provides relevant data about the role of HOP in auxin regulation in plants and uncovers that both co-chaperones, SGT1b and HOP, cooperate in the stabilisation of common targets involved in plant development.


Subject(s)
Arabidopsis Proteins , Arabidopsis , F-Box Proteins , Animals , Arabidopsis/metabolism , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , F-Box Proteins/metabolism , HSP90 Heat-Shock Proteins/metabolism , Indoleacetic Acids/metabolism , Mammals/metabolism , Molecular Chaperones/metabolism , Receptors, Cell Surface/metabolism
5.
Plant Physiol ; 187(3): 1679-1689, 2021 11 03.
Article in English | MEDLINE | ID: mdl-34618051

ABSTRACT

HOPs (HSP70-HSP90 organizing proteins) are a highly conserved family of HSP70 and HSP90 co-chaperones whose role in assisting the folding of various hormonal receptors has been extensively studied in mammals. In plants, HOPs are mainly associated with stress response, but their potential involvement in hormonal networks remains completely unexplored. In this article we describe that a member of the HOP family, HOP3, is involved in the jasmonic acid (JA) pathway and is linked to plant defense responses not only to pathogens, but also to a generalist herbivore. The JA pathway regulates responses to Botrytis cinerea infection and to Tetranychus urticae feeding; our data demonstrate that the Arabidopsis (Arabidopsis thaliana) hop3-1 mutant shows an increased susceptibility to both. The hop3-1 mutant exhibits reduced sensitivity to JA derivatives in root growth assays and downregulation of different JA-responsive genes in response to methyl jasmonate, further revealing the relevance of HOP3 in the JA pathway. Interestingly, yeast two-hybrid assays and in planta co-immunoprecipitation assays found that HOP3 interacts with COI1, suggesting that COI1 is a target of HOP3. Consistent with this observation, COI1 activity is reduced in the hop3-1 mutant. All these data strongly suggest that, specifically among HOPs, HOP3 plays a relevant role in the JA pathway by regulating COI1 activity in response to JA and, consequently, participating in defense signaling to biotic stresses.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/physiology , Cyclopentanes/pharmacology , Molecular Chaperones/genetics , Oxylipins/pharmacology , Plant Growth Regulators/pharmacology , Arabidopsis/genetics , Arabidopsis Proteins/metabolism , Molecular Chaperones/metabolism , Signal Transduction
6.
Plant J ; 107(1): 256-267, 2021 07.
Article in English | MEDLINE | ID: mdl-33899980

ABSTRACT

Mutations in the eukaryotic translation initiation factors eIF4E and eIF(iso)4E confer potyvirus resistance in a range of plant hosts. This supports the notion that, in addition to their role in translation of cellular mRNAs, eIF4E isoforms are also essential for the potyvirus cycle. CERES is a plant eIF4E- and eIF(iso)4E-binding protein that, through its binding to the eIF4Es, modulates translation initiation; however, its possible role in potyvirus resistance is unknown. In this article, we analyse if the ectopic expression of AtCERES is able to interfere with turnip mosaic virus replication in plants. Our results demonstrate that, during infection, the ectopic expression of CERES in Nicotiana benthamiana promotes the development of a mosaic phenotype when it is accumulated to moderate levels, but induces veinal necrosis when it is accumulated to higher levels. This necrotic process resembles a hypersensitive response (HR)-like response that occurs with different HR hallmarks. Remarkably, Arabidopsis plants inoculated with a virus clone that promotes high expression of CERES do not show signs of infection. These final phenotypical outcomes are independent of the capacity of CERES to bind to eIF4E. All these data suggest that CERES, most likely due to its leucine-rich repeat nature, could act as a resistance protein, able to promote a range of different defence responses when it is highly overexpressed from viral constructs.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/virology , Eukaryotic Initiation Factors/genetics , Nicotiana/genetics , Nicotiana/virology , Plant Diseases/virology , Arabidopsis/genetics , Arabidopsis Proteins/metabolism , Eukaryotic Initiation Factor-4E/genetics , Eukaryotic Initiation Factor-4E/metabolism , Eukaryotic Initiation Factors/metabolism , Gene Expression Regulation, Plant , Host-Pathogen Interactions/genetics , Host-Pathogen Interactions/physiology , Necrosis , Phenotype , Plant Leaves/virology , Plants, Genetically Modified , Potyvirus/pathogenicity , Potyvirus/physiology , Protein Isoforms/metabolism , Virus Replication
7.
Front Plant Sci ; 11: 591940, 2020.
Article in English | MEDLINE | ID: mdl-33193548

ABSTRACT

Protein folding is an essential step for protein functionality. In eukaryotes this process is carried out by multiple chaperones that act in a cooperative manner to maintain the proteome homeostasis. Some of these chaperones are assisted during protein folding by different co-chaperones. One of these co-chaperones is HOP, the HSP70-HSP90 organizing protein. This assistant protein, due to its importance, has been deeply analyzed in other eukaryotes, but its function has only recently started to be envisaged in plants. In this kingdom, the role of HOP has been associated to plant response to different cellular, biotic and abiotic stresses. In this article, we analyze the current knowledge about HOP in eukaryotes, paying a special attention to the recently described roles of HOP in plants. In addition, we discuss the recent breakthroughs in the field and the possible new avenues for the study of plant HOP proteins in the future.

8.
Int J Mol Sci ; 21(19)2020 Oct 04.
Article in English | MEDLINE | ID: mdl-33020405

ABSTRACT

Translation plays an important role in plant adaptation to different abiotic and biotic stresses; however, the mechanisms involved in translational regulation during each specific response and their effect in translation are poorly understood in plants. In this work, we show that GCN2 promotes eIF2α phosphorylation upon contact with Botrytis cinerea spores, and that this phosphorylation is required for the proper establishment of plant defense against the fungus. In fact, independent gcn2 mutants display an enhanced susceptibility to B. cinerea infection, which is highlighted by an increased cell death and reduced expression of ethylene- and jasmonic-related genes in the gcn2 mutants. eIF2α phosphorylation is not only triggered in the presence of the fungus, but interestingly, is also achieved in the sole presence of the microbe-associated molecular pattern (MAMP) chitin. Moreover, analysis of de novo protein synthesis by 35SMet-35SCys incorporation indicates that chitin treatment promotes a global inhibition of translation. Taken together, these results suggest that eIF2α phosphorylation by GCN2 is promoted in the presence of chitin and plays an important role in plant defense against B. cinerea infection.


Subject(s)
Arabidopsis Proteins/genetics , Disease Resistance/genetics , Eukaryotic Initiation Factor-2/genetics , Plant Diseases/genetics , Protein Kinases/genetics , Arabidopsis/genetics , Arabidopsis/growth & development , Botrytis/pathogenicity , Chitin/genetics , Ethylenes/metabolism , Gene Expression Regulation, Plant/genetics , Phosphorylation/genetics , Plant Diseases/microbiology
9.
Bio Protoc ; 10(16): e3713, 2020 Aug 20.
Article in English | MEDLINE | ID: mdl-33659377

ABSTRACT

The nucleotides involved in RNA-RNA interaction can be tagged by chemical- or UV-induced crosslinking, and further identified by classical or modern high throughput techniques. The contacts of mRNA with 18S rRNA that occur along the mRNA channel of 40S subunit have been mapped by site-specific UV crosslinking followed by reverse transcriptase termination sites (RTTS) using radioactive or fluorescent oligonucleotides. However, the sensitivity of this technique is restricted to the detection of those fragments that resulted from the most frequent crosslinkings. Here, we combined RTTS with RNAseq to map the mRNA-18S rRNA contacts with a much deeper resolution. Although aimed to detect the interaction of mRNA with the ES6S region of 18S rRNA, this technique can also be applied to map the interaction of mRNA with other non-coding RNA molecules (e.g., snRNAs, microRNAs and lncRNAs) during transcription, splicing or RNA-mediated postranscriptional regulation.

10.
J Virol ; 94(3)2020 01 17.
Article in English | MEDLINE | ID: mdl-31723025

ABSTRACT

Alphaviruses are insect-borne viruses that alternate between replication in mosquitoes and vertebrate species. Adaptation of some alphaviruses to vertebrate hosts has involved the acquisition of an RNA structure (downstream loop [DLP]) in viral subgenomic mRNAs that confers translational resistance to protein kinase (PKR)-mediated eIF2α phosphorylation. Here, we found that, in addition to promoting eIF2-independent translation of viral subgenomic mRNAs, presence of the DLP structure also increased the resistance of alphavirus to type I interferon (IFN). Aura virus (AURAV), an ecologically isolated relative of Sindbis virus (SV) that is poorly adapted to replication in vertebrate cells, displayed a nonfunctional DLP structure and dramatic sensitivity to type I IFN. Our data suggest that an increased resistance to IFN emerged during translational adaptation of alphavirus mRNA to vertebrate hosts, reinforcing the role that double-stranded RNA (dsRNA)-activated protein kinase (PKR) plays as both a constitutive and IFN-induced antiviral effector. Interestingly, a mutant SV lacking the DLP structure (SV-ΔDLP) and AURAV both showed a marked oncotropism for certain tumor cell lines that have defects in PKR expression and/or activation. AURAV selectively replicated in and killed some cell lines derived from human hepatocarcinoma (HCC) that lacked PKR response to infection or poly(I·C) transfection. The oncolytic activities of SV-ΔDLP and AURAV were also confirmed using tumor xenografts in mice, showing tumor regression activities comparable to wild-type SV. Our data show that translation of alphavirus subgenomic mRNAs plays a central role in IFN susceptibility and cell tropism, suggesting an unanticipated oncolytic potential that some naive arboviruses may have in virotherapy.IMPORTANCE Interferons (IFNs) induce the expression of a number of antiviral genes that protect the cells of vertebrates against viruses and other microbes. The susceptibility of cells to viruses greatly depends on the level and activity of these antiviral effectors but also on the ability of viruses to counteract this antiviral response. Here, we found that the level of one of the main IFN effectors in the cell, the dsRNA-activated protein kinase (PKR), greatly determines the permissiveness of cells to alphaviruses that lack mechanisms to counteract its activation. These naive viruses also showed a hypersensitivity to IFN, suggesting that acquisition of IFN resistance (even partial) has probably been involved in expanding the host range of alphaviruses in the past. Interestingly, some of these naive viruses showed a marked oncotropism for some tumor cell lines derived from human hepatocarcinoma (HCC), opening the possibility of their use in oncolytic therapy to treat human tumors.


Subject(s)
Alphavirus/genetics , Alphavirus/metabolism , Eukaryotic Initiation Factor-2/metabolism , Interferons/metabolism , Protein Kinases/metabolism , RNA, Double-Stranded/metabolism , Animals , Antiviral Agents/pharmacology , Apoptosis , Carcinoma, Hepatocellular , Cell Line, Tumor , Female , Humans , Liver Neoplasms , Mice, SCID , Phosphorylation , RNA, Messenger/metabolism , Sindbis Virus/genetics , Vertebrates/genetics , Virus Replication/drug effects
11.
Nat Plants ; 5(12): 1283-1296, 2019 12.
Article in English | MEDLINE | ID: mdl-31819221

ABSTRACT

Translation is a fundamental step in gene expression that regulates multiple developmental and stress responses. One key step of translation initiation is the association between eIF4E and eIF4G. This process is regulated in different eukaryotes by proteins that bind to eIF4E; however, evidence of eIF4E-interacting proteins able to regulate translation is missing in plants. Here, we report the discovery of CERES, a plant eIF4E-interacting protein. CERES contains an LRR domain and a canonical eIF4E-binding site. Although the CERES-eIF4E complex does not include eIF4G, CERES forms part of cap-binding complexes, interacts with eIF4A, PABP and eIF3, and co-sediments with translation initiation complexes in vivo. Moreover, CERES promotes translation in vitro and general translation in vivo, while it modulates the translation of specific mRNAs related to light and carbohydrate response. These data suggest that CERES is a non-canonical translation initiation factor that modulates translation in plants.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Eukaryotic Initiation Factor-4E/metabolism , Eukaryotic Initiation Factors/metabolism , Protein Biosynthesis , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Binding Sites , Eukaryotic Initiation Factor-4E/genetics , Eukaryotic Initiation Factor-4G/genetics , Eukaryotic Initiation Factor-4G/metabolism , Eukaryotic Initiation Factors/genetics , Protein Binding , Protein Domains , RNA, Messenger/genetics
12.
Elife ; 82019 12 02.
Article in English | MEDLINE | ID: mdl-31789591

ABSTRACT

Loading of mRNA onto the ribosomal 43S pre-initiation complex (PIC) and its subsequent scanning require the removal of the secondary structure of the by RNA helicases such as eIF4A. However, the topology and mechanics of the scanning complex bound to mRNA (48S-PIC) and the influence of its solvent-side composition on the scanning process are poorly known. Here, we found that the ES6S region of the 48S-PIC constitutes an extended binding channel for eIF4A-mediated unwinding of mRNA and scanning. Blocking ES6S inhibited the cap-dependent translation of mRNAs that have structured 5' UTRs (including G-quadruplexes), many of which are involved in signal transduction and growth, but it did not affect IRES-driven translation. Genome-wide analysis of mRNA translation revealed a great diversity in ES6S-mediated scanning dependency. Our data suggest that mRNA threading into the ES6S region makes scanning by 48S PIC slower but more processive. Hence, we propose a topological and functional model of the scanning 48S-PIC.


Subject(s)
Eukaryotic Initiation Factor-4F/genetics , RNA Helicases/chemistry , RNA, Messenger/genetics , Saccharomyces cerevisiae Proteins/genetics , Transduction, Genetic , 5' Untranslated Regions/genetics , G-Quadruplexes , Peptide Chain Initiation, Translational/genetics , Protein Biosynthesis/genetics , RNA Helicases/genetics , RNA, Messenger/chemistry , Ribosomes/genetics , Saccharomyces cerevisiae/genetics
13.
Plant J ; 99(6): 1203-1219, 2019 09.
Article in English | MEDLINE | ID: mdl-31111599

ABSTRACT

Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5' untranslated regions (5'-UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Plant Roots/metabolism , Polyadenylation/genetics , Salt Stress/genetics , mRNA Cleavage and Polyadenylation Factors/metabolism , 5' Untranslated Regions , Abscisic Acid/metabolism , Alleles , Arabidopsis/drug effects , Arabidopsis/growth & development , Arabidopsis Proteins/genetics , Cadmium/toxicity , Cell Division/genetics , Gene Expression Regulation, Plant/genetics , Mutation , Phenotype , Plant Roots/cytology , Plant Roots/drug effects , Plant Roots/genetics , Polyadenylation/drug effects , Protein Biosynthesis/genetics , RNA, Messenger/genetics , RNA, Messenger/metabolism , mRNA Cleavage and Polyadenylation Factors/genetics
14.
Plant Cell Environ ; 41(8): 1852-1869, 2018 08.
Article in English | MEDLINE | ID: mdl-29740845

ABSTRACT

HSP70-HSP90 organizing protein (HOP) is a family of cytosolic cochaperones whose molecular role in thermotolerance is quite unknown in eukaryotes and unexplored in plants. In this article, we describe that the three members of the AtHOP family display a different induction pattern under heat, being HOP3 highly regulated during the challenge and the attenuation period. Despite HOP3 is the most heat-regulated member, the analysis of the hop1 hop2 hop3 triple mutant demonstrates that the three HOP proteins act redundantly to promote long-term acquired thermotolerance in Arabidopsis. HOPs interact strongly with HSP90 and part of the bulk of HOPs shuttles from the cytoplasm to the nuclei and to cytoplasmic foci during the challenge. RNAseq analyses demonstrate that, although the expression of the Hsf targets is not generally affected, the transcriptional response to heat is drastically altered during the acclimation period in the hop1 hop2 hop3 triple mutant. This mutant also displays an unusual high accumulation of insoluble and ubiquitinated proteins under heat, which highlights the additional role of HOP in protein quality control. These data reveal that HOP family is involved in different aspects of the response to heat, affecting the plant capacity to acclimate to high temperatures for long periods.


Subject(s)
Arabidopsis Proteins/physiology , Arabidopsis/physiology , Molecular Chaperones/physiology , Thermotolerance , Blotting, Western , Gene Expression Regulation, Plant , Glucuronidase/metabolism , Polymerase Chain Reaction , Sequence Analysis, RNA
15.
Nucleic Acids Res ; 46(8): 4176-4187, 2018 05 04.
Article in English | MEDLINE | ID: mdl-29415133

ABSTRACT

The topology and dynamics of the scanning ribosomal 43S pre-initiation complex (PIC) bound to mRNA and initiation factors (eIFs) are probably the least understood aspects of translation initiation in eukaryotes. Recently, we described a trapping mechanism in alphavirus that stalls the PIC during scanning of viral mRNA. Using this model, we were able to snapshot for the first time the eIF4A helicase bound to mRNA in a 48S initiation complex assembled in vitro. This interaction was only detected in the presence of the natural stem loop structure (DLP) located downstream from the AUG in viral mRNA that promoted stalling of the PIC, suggesting that DLP stability was enough to jam the helicase activity of eIF4A in a fraction of assembled 48S complexes. However, a substantial proportion of DLP mRNA molecules were effectively unwound by eIF4A in vitro, an activity that alphaviruses counteract in infected cells by excluding eIF4A from viral factories. Our data indicated that eIF4A-mRNA contact occurred in (or near) the ES6S region of the 40S subunit, suggesting that incoming mRNA sequences penetrate through the ES6S region during the scanning process. We propose a topological model of the scanning PIC and how some viruses have exploited this topology to translate their mRNAs with fewer eIF requirements.


Subject(s)
Alphavirus/genetics , Eukaryotic Initiation Factor-4A/chemistry , Peptide Chain Initiation, Translational , RNA, Messenger/chemistry , RNA, Viral/chemistry , Ribosome Subunits, Small, Eukaryotic/chemistry , Animals , Cell Line , Eukaryotic Initiation Factor-4A/metabolism , Models, Molecular , RNA, Messenger/metabolism , RNA, Viral/metabolism , Ribosome Subunits, Small, Eukaryotic/metabolism
16.
RNA Biol ; 13(12): 1223-1227, 2016 12.
Article in English | MEDLINE | ID: mdl-27824302

ABSTRACT

Location of the translation initiation codon generally requires scanning of the 43S ribosomal preinitiation complex (43S PIC) from the 5' of the mRNA. Associated RNA helicases can facilitate movement of the 43S PIC by removing secondary structure present in the 5' UTR of mRNA, which is required for codon inspection. The canonical RNA-dependent helicase eIF4A is directly involved in this process, as part of the eIF4F complex (eIF4G + eIF4A + eIF4E) that associates first with mRNA and then recruits the 43S PIC to initiate scanning. The topology and operational mechanism of the scanning PIC are probably the least understood aspects of the initiation step. Recent findings from translation of alphavirus mRNA, together with new biochemical and structural data of the 43S PIC, suggest a role for the ES6S region of 40S as the gateway for mRNA entry during scanning. The presence of eIF4G-eIF4A complex in this region, interacting with the incoming mRNA, supports a model where eIF4A could work ahead of the scanning complex during translation initiation. Here we present additional data supporting this model.


Subject(s)
RNA, Messenger/chemistry , Ribosomes/metabolism , Viral Proteins/metabolism , Viruses/metabolism , Eukaryotic Initiation Factor-4A/metabolism , Eukaryotic Initiation Factor-4E/metabolism , Eukaryotic Initiation Factor-4G/metabolism , Models, Molecular , Protein Biosynthesis , RNA, Messenger/genetics , RNA, Viral/chemistry , RNA, Viral/genetics , Viruses/genetics
17.
Nucleic Acids Res ; 44(9): 4368-80, 2016 05 19.
Article in English | MEDLINE | ID: mdl-26984530

ABSTRACT

During translation initiation, eukaryotic initiation factor 2 (eIF2) delivers the Met-tRNA to the 40S ribosomal subunit to locate the initiation codon (AUGi) of mRNA during the scanning process. Stress-induced eIF2 phosphorylation leads to a general blockade of translation initiation and represents a key antiviral pathway in mammals. However, some viral mRNAs can initiate translation in the presence of phosphorylated eIF2 via stable RNA stem-loop structures (DLP; Downstream LooP) located in their coding sequence (CDS), which promote 43S preinitiation complex stalling on the initiation codon. We show here that during the scanning process, DLPs of Alphavirus mRNA become trapped in ES6S region (680-914 nt) of 18S rRNA that are projected from the solvent side of 40S subunit. This trapping can lock the progress of the 40S subunit on the mRNA in a way that places the upstream initiator AUGi on the P site of 40S subunit, obviating the participation of eIF2. Notably, the DLP structure is released from 18S rRNA upon 60S ribosomal subunit joining, suggesting conformational changes in ES6Ss during the initiation process. These novel findings illustrate how viral mRNA is threaded into the 40S subunit during the scanning process, exploiting the topology of the 40S subunit solvent side to enhance its translation in vertebrate hosts.


Subject(s)
Alphavirus/genetics , Peptide Chain Initiation, Translational , RNA, Messenger/genetics , RNA, Viral/genetics , Aedes , Alphavirus/metabolism , Animals , Base Sequence , Cell Line , Codon, Initiator , Cricetinae , Gene Expression Regulation, Viral , Inverted Repeat Sequences , Models, Molecular , RNA Stability , RNA, Messenger/chemistry , RNA, Messenger/metabolism , RNA, Ribosomal, 18S/chemistry , RNA, Ribosomal, 18S/physiology , RNA, Viral/chemistry , RNA, Viral/metabolism , Ribosomes/physiology
18.
PLoS One ; 6(2): e16711, 2011 Feb 02.
Article in English | MEDLINE | ID: mdl-21311764

ABSTRACT

Most viruses express during infection products that prevent or neutralize the effect of the host dsRNA activated protein kinase (PKR). Translation of Sindbis virus (SINV) mRNA escapes to PKR activation and eIF2 phosphorylation in infected cells by a mechanism that requires a stem loop structure in viral 26S mRNA termed DLP to initiate translation in the absence of functional eIF2. Unlike the rest of viruses tested, we found that Alphavirus infection allowed a strong PKR activation and eIF2α phosphorylation in vitro and in infected animals so that the presence of DLP structure in mRNA was critical for translation and replication of SINV. Interestingly, infection of MEFs with some viruses that express PKR inhibitors prevented eIF2α phosphorylation after superinfection with SINV, suggesting that viral anti-PKR mechanisms could be exchangeable. Thus, translation of SINV mutant lacking the DLP structure (ΔDLP) in 26S mRNA was partially rescued in cells expressing vaccinia virus (VV) E3 protein, a known inhibitor of PKR. This case of heterotypic complementation among evolutionary distant viruses confirmed experimentally a remarkable case of convergent evolution in viral anti-PKR mechanisms. Our data reinforce the critical role of PKR in regulating virus-host interaction and reveal the versatility of viruses to find different solutions to solve the same conflict.


Subject(s)
Evolution, Molecular , Genetic Variation , Host-Pathogen Interactions/genetics , Viruses/genetics , eIF-2 Kinase/antagonists & inhibitors , Animals , Base Sequence , Cells, Cultured , Genetic Variation/physiology , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Models, Biological , Molecular Sequence Data , Nucleic Acid Conformation , Signal Transduction/genetics , Sindbis Virus/genetics , Sindbis Virus/metabolism , Virus Replication/genetics , Viruses/metabolism , Viruses/pathogenicity , eIF-2 Kinase/genetics
19.
Proc Natl Acad Sci U S A ; 107(21): 9837-42, 2010 May 25.
Article in English | MEDLINE | ID: mdl-20457920

ABSTRACT

Infection of cultured cells with lytic animal viruses often results in the selective inhibition of host protein synthesis, whereas viral mRNA is efficiently translated under these circumstances. This phenomenon, known as "shut off," has been well described at the molecular level for some viruses, but there is not yet any direct or indirect evidence supporting the idea that it also should operate in animals infected with viruses. To address this issue, we constructed recombinant Sindbis virus (SV)-expressing reporter mRNA, the translation of which is sensitive or resistant to virus-induced shut off. As found in cultured cells, replication of SV in mouse brain was associated with a strong phosphorylation of eukaryotic initiation factor (eIF2) that prevented translation of reporter mRNA (luciferase and EGFP). Translation of these reporters was restored in vitro, in vivo, and ex vivo when a viral RNA structure, termed downstream hairpin loop, present in viral 26S mRNA, was placed at the 5' end of reporter mRNAs. By comparing the expression of shut off-sensitive and -resistant reporters, we unequivocally concluded that replication of SV in animal tissues is associated with a profound inhibition of nonviral mRNA translation. A strategy as simple as that followed here might be applicable to other viruses to evaluate their interference on host translation in infected animals.


Subject(s)
Alphavirus Infections/virology , Protein Biosynthesis , Sindbis Virus/physiology , Viral Proteins/genetics , Animals , Brain/virology , Cell Line , Cricetinae , Eukaryotic Initiation Factor-2/metabolism , Female , Genes, Reporter , Mice , RNA, Messenger/genetics , Rats , Sindbis Virus/genetics , Virus Replication
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