ABSTRACT
Cells derived from human skin malignant melanoma were implanted subcutaneously in athymic nude mice. Tumors which developed at the implant site were treated with ultrasonically induced hyperthermia at 49 degrees C for 30 min. Tumors were scanned with a computerized diagnostic ultrasound system before and after treatment. Light (LM) and electron (EM) micrographs of tumors were obtained after scanning. Changes in ultrasonic tissue characterization parameters following treatment were well correlated with histopathologic changes observed in tumors. The results are significant in terms of clinical application of ultrasonically induced hyperthermia for treatment of intraocular tumors and the noninvasive monitoring of tumors by use of diagnostic ultrasound.
Subject(s)
Melanoma, Experimental/pathology , Skin Neoplasms/pathology , Ultrasonic Therapy , Animals , Humans , Image Interpretation, Computer-Assisted , Melanoma, Experimental/therapy , Mice , Mice, Nude , Microscopy, Electron , Neoplasm Transplantation , Skin Neoplasms/therapy , Spectrum AnalysisABSTRACT
High-intensity focused ultrasound was employed to seal lens capsular tears in a rabbit model. Ultrasound therapy was applied either contiguously, thereby completely covering the tear, or in a discrete exposure pattern around the tear. Both methods prevented the formation of a generalised cataract. This was in contrast to results observed in a group of control (untreated) animals which all developed generalised lens opacities. Each control animal also developed a local lens opacity at the site of the capsular tear, as did half the animals treated with the discrete pattern. No animal treated with contiguous exposures developed any local or generalised traumatic-type cataract other than the small lens opacity immediately produced by the treatment. These treatment cataracts would not constitute a significant impediment to vision so long as they did not fall on the visual axis.
Subject(s)
Lens Capsule, Crystalline/injuries , Lens, Crystalline/injuries , Ultrasonic Therapy , Animals , Cataract/pathology , Cataract/prevention & control , Lens Capsule, Crystalline/pathology , RabbitsABSTRACT
Human intraocular tumors and tumors derived from human tumor cell lines grown subcutaneously in the athymic nude mouse were scanned by diagnostic ultrasound. Radiofrequency scan data were converted to digital form and analyzed in the frequency domain. Characteristics of normalized power spectra were found to be significantly different among human spindle cell malignant melanomas, mixed/epithelioid malignant melanomas, metastatic carcinomas, and hemangiomas. Significant differences, as well, were found between implanted primary skin malignant melanomas and adenocarcinomas of the lung, colon, and stomach. Comparison of spectral properties of human intraocular and implanted tumors revealed that human spindle cell malignant melanomas and implanted melanomas exhibit similar characteristics. Human intraocular metastatic tumors from the lung were found to exhibit characteristics similar to those of implanted lung tumors. These results indicate that the implantation of human tumor cell lines in the nude mouse may provide a very useful model for application of diagnostic and therapeutic ultrasound modalities to human intraocular tumors.
Subject(s)
Acoustics , Eye Neoplasms/diagnosis , Melanoma/diagnosis , Ultrasonography , Adenocarcinoma/diagnosis , Animals , Cell Line , Cell Separation , Colonic Neoplasms/pathology , Computers , Disease Models, Animal , Humans , Lung Neoplasms/pathology , Mice , Mice, Nude , Neoplasm Transplantation , Skin Neoplasms/pathology , Spectrum AnalysisABSTRACT
Focused, high-intensity therapeutic ultrasound was used to treat 69 selected patients with uncontrollably elevated intraocular pressure (IOP). This new technique selectively thins scleral collagen, and produces focal damage to the ciliary epithelium. These tissue modifications provide a reduction in IOP pressure to 25 mmHg or less in 83% of patients with a minimum three-month follow-up period.
