Calidad de vida laboral en empleados de una empresa distribuidora de bebidas de Santander, Colombia / Quality of work life in employees of beverage distribution company in Santander, Colombia

Introducción: Es deber de las organizaciones preocuparse por el bienestar físico, mental y social de los trabajadores. Evaluar la calidad de vida laboral puede indicar cómo el empleo cubre las necesidades de seguridad, satisfacción, bienestar y desarrollo personal/laboral del trabajador. Método: estudio transversal en 122 trabajadores con labores de conducción de vehículos, cargue/descargue, entrega/recepción de mercancía y dinero, seleccionados a conveniencia en una empresa distribuidora de bebidas. La calidad de vida laboral se evaluó mediante CVT-GOHISALO y se exploró su relación con variables sociodemográficas y laborales. Resultados: se encontró un alto nivel de satisfacción en el soporte institucional para el trabajo y la seguridad en el trabajo, un nivel medio en la integración al puesto y el desarrollo personal, un nivel bajo en la satisfacción a través del trabajo y un nivel de satisfacción en riesgo en el bienestar logrado y la administración del tiempo libre. La calidad de vida laboral se relacionó con el número de horas y días trabajados, también con la antigüedad en las dimensiones de integración al puesto y seguridad en el trabajo; el nivel educativo, la jornada laboral, las pausas activas y el cargo se relacionaron con todas las dimensiones excepto con soporte institucional y administración del tiempo libre. Conclusiones: La calidad de vida laboral fue valorada por cómo el empleador brinda los elementos e indicaciones necesarias para realizar el trabajo; pero con deficiencias en el bienestar y el manejo del tiempo. La calidad de vida fue más baja en trabajadores con secundaria, que laboran más días/horas y en la jornada diurna, tienen mayor antigüedad, son conductores y no realizan pausas activas. (AU)

Introduction: Organizations have to care about the physical, mental, and social well-being of their workers. The evaluation of the quality of work-life indicates how the job responds to the needs of safety, satisfaction, well-being, and personal and professional development of the worker. Method: a cross-sectional study in 122 workers with occupations of driving vehicles, loading, unloading, delivering merchandise, and receiving money, selected at convenience in a beverage distribution company. We evaluate the quality of work-life using CVT-GOHISALO and we expose its relationship with sociodemographic and work variables using Pearson’s correlation coefficient and Student’s T-test and ANOVA. Results: We found a high level of satisfaction in institutional support for work and job security, a medium level in job integration and personal development, a low level of job satisfaction, and a level of satisfaction at risk in well-being achieved and management of free time. The quality of working life was related to the number of hours and days worked, also to seniority in the dimensions of job integration and job security; educational level, working hours, active breaks, and position were related to all dimensions except institutional support and free time management. Conclusions: The quality of working life was assessed by how the employer provides the elements and indications necessary to carry out the work, but with deficiencies in well-being and time management. The quality of life was lower in workers with secondary education, who work more days/hours and during the day, have more seniority, are drivers, and do not take active breaks. (AU)

Metabolic shift in the production of corrinoid compounds by Lactobacillus coryniformis in the absence of purines.

Lactobacillus coryniformis CRL 1001 and L. reuteri CRL 1098 have the complete genes necessary to synthesize pseudo-cobalamin as final product in a vitamin B12 free commercial medium. Unlike vitaminB12 (the most biologically active form), the pseudo-cobalamin contains adenine instead of 5,6-dimethlbenzimidazole (DMB) in the Coα-ligand. Considering the vitamin B12-gene clusters of these bacteria, the aim of this work was to analyze the production of corrinoids with DMB (vitamin B12) instead of adenine (pseudo-B12) as lower ligand base in a vitamin B12 free chemically defined medium (CDM) without purines. Genome-wide screening of genes related to purine metabolism showed that both strains possess all pur genes necessary for the synthesis of inositol monophosphate, the main precursor for purine biosynthesis. Accordingly, both strains were able to grow in B12 free CDM without purines, with the supplementation of different synthetic intermediaries. Isolated compounds with positive vitamin B12 activity were quantified and characterized by LC/MS-MS. Total corrinoids values were higher for both strains in comparison to those obtained in vitaminB12 free commercial medium. Interestingly, CRL 1001 strain synthesized cobalamin, suggesting that this strain is able to activate DMB as nitrogenous base instead adenine when it is in excess in a purine-free medium. The present paper represents the first demonstration of a partial metabolic shift to produce vitamin B12 in a Lactobacillus strain.

Novel Pathway for Corrinoid Compounds Production in Lactobacillus.

Vitamin B12 or cobalamin is an essential metabolite for humans, which makes it an interesting compound for many research groups that focus in different producer-strains synthesis pathways. In this work, we report the influence of key intermediaries for cobalamin synthesis added to the culture medium in two Lactobacillus (L.) strains, L. reuteri CRL 1098 and L. coryniformis CRL 1001. Here, we report that addition of Co2+ and 5,6-dimethylbenzimidazole increased the corrinoid compounds production in both strains while addition of L-threonine increased only the corrinoid compounds production by CRL 1001 strain. Then, we purified and characterized by LC-MS the corrinoid compounds obtained. Physiological studies besides in silico analysis revealed that L. reuteri CRL 1098 and L. coryniformis CRL 1001 follow different pathways for the last steps of the corrinoid compounds synthesis.

Cobalamin production by Lactobacillus coryniformis: biochemical identification of the synthetized corrinoid and genomic analysis of the biosynthetic cluster.

BACKGROUND: Despite the fact that most vitamins are present in a variety of foods, malnutrition, unbalanced diets or insufficient intake of foods are still the cause of vitamin deficiencies in humans in some countries. Vitamin B12 (Cobalamin) is a complex compound that is only naturally produced by bacteria and archea. It has been reported that certain strains belonging to lactic acid bacteria group are capable of synthesized water-soluble vitamins such as those included in the B-group, as vitamin B12. In this context, the goal of the present paper was to evaluate and characterize the production of vitamin B12 in Lactobacillus coryniformis CRL 1001, a heterofermentative strain isolated from silage. RESULTS: Cell extract of L. coryniformis CRL 1001, isolated from silage, is able to correct the coenzyme B12 requirement of Salmonella enterica serovar Typhimurium AR 2680 in minimal medium. The chemical characterization of the corrinoid-like molecule isolated from CRL 1001 cell extract using HPLC and mass spectrometry is reported. The majority of the corrinoid produced by this strain has adenine like Coα-ligand instead 5,6-dimethylbenzimidazole. Genomic studies revealed the presence of the complete machinery of the anaerobic biosynthesis pathway of coenzyme B12. The detected genes encode all proteins for the corrin ring biosynthesis and for the binding of upper (ß) and lower (α) ligands in one continuous stretch of the chromosome. CONCLUSIONS: The results here described show for the first time that L. coryniformis subsp. coryniformis CRL 1001 is able to produce pseudocobalamin containing adenine instead of 5,6-dimethlbenzimidazole in the Coα-ligand. Genomic analysis allowed the identification and characterization of the complete de novo biosynthetic pathway of the corrinoid produced by the CRL 1001 strain.