Engineering intraporous solvent environments: effects of aqueous-organic solvent mixtures on competition between zeolite-catalyzed epoxidation and H2O2 decomposition pathways.

Solvent molecules alter the free energies of liquid phase species and adsorbed intermediates during catalytic reactions, thereby impacting rates and selectivities. Here, we examine these effects through the epoxidation of 1-hexene (C6H12) with hydrogen peroxide (H2O2) over hydrophilic and hydrophobic Ti-BEA zeolites immersed in aqueous solvent mixtures (acetonitrile, methanol, and γ-butyrolactone). Greater H2O mole fractions provide greater epoxidation rates, lower H2O2 decomposition rates, and hence improved H2O2 selectivities to the desired epoxide product in each combination of solvent and zeolite. The mechanisms for epoxidation and H2O2 decomposition remain constant across solvent compositions; however, H2O2 activates reversibly in protic solutions. Differences in rates and selectivities reflect the disproportionate stabilization of transition states within zeolite pores with respect to surface intermediates and fluid phase reactants, as evinced by turnover rates normalized by the activity coefficients of C6H12 and H2O2. Opposing trends in activation barriers suggest that the hydrophobic epoxidation transition state disrupts hydrogen bonds with solvent molecules, while the hydrophilic decomposition transition state forms hydrogen bonds with surrounding solvent molecules. Solvent compositions and adsorption volumes within pores, from 1H NMR spectroscopy and vapor adsorption, depend on the composition of the bulk solution and the density of silanol defects within pores. Strong correlations between epoxidation activation enthalpies and epoxide adsorption enthalpies from isothermal titration calorimetry indicate that the reorganization of solvent molecules (and associated entropy gains) required to accommodate transition states provides the most significant contribution to the stability of transition states that determine rates and selectivities. These results demonstrate that replacing a portion of organic solvents with H2O offers opportunities to increase rates and selectivities for zeolite-catalyzed reactions while reducing usage of organic solvents for chemical manufacturing.

Extending the Bioavailability of Hydrophilic Antioxidants for Metal Ion Detoxification via Crystallization with Polysaccharide Dopamine.

Although metal ions, such as silver and gold, have been shown to have strong antimicrobial properties, their potential to have toxic effects on human and environmental health has gained interest with an improved understanding of their mechanisms to promote oxidative stress. Redox control is a major focus of many drug delivery systems and often incorporates an antioxidant as the active pharmaceutical ingredient (API) to neutralize overproduced reactive oxygen species (ROS). Nevertheless, there are still limitations with bioavailability and extended redox control with regard to antioxidant drug delivery. Herein, this study develops a colloidal antioxidant crystal system that dissolves sustainably through polymer stabilization using sodium hyaluronate conjugated with dopamine (HA-dopa). We explore the role of dopamine incorporation into crystal-stabilizing polymers and quantify the balance between drug-polymer interactions and competing polymer-polymer interactions. We propose that this type of analysis is useful in the engineering of and provides insight into the release behavior of polymer-crystal complexes. In developing our crystal complex, N-acetylcysteine (NAC) was used as the model antioxidant to protect against silver ion toxicity. We found that our optimized HA-dopa-stabilized NAC crystals prolong the release time of NAC 5-fold compared to a polymer-free NAC crystal. Therefore, following sublethal exposure to AgNO3, the extended lifetime of NAC was able to maintain normal intracellular ROS levels, modulate metabolic function, mitigate fluctuations in ATP levels and ATP synthase activity, and preserve contraction frequency in engineered cardiac muscle tissue. Furthermore, the protective effects of the HA-dopa-stabilized NAC crystals were extended to a Daphnia magna model where silver-ion-induced change to both cell-level biochemistry and organ function was alleviated. As such, we propose that the packaging of hydrophilic antioxidants as colloidal crystals drastically extends the lifetime of the API, better maintains ROS homeostasis post metal ion exposure, and therefore preserves both intracellular biochemistry and tissue functionality.

Influence of solvent structure and hydrogen bonding on catalysis at solid-liquid interfaces.

Solvent molecules interact with reactive species and alter the rates and selectivities of catalytic reactions by orders of magnitude. Specifically, solvent molecules can modify the free energies of liquid phase and surface species via solvation, participating directly as a reactant or co-catalyst, or competitively binding to active sites. These effects carry consequences for reactions relevant for the conversion of renewable or recyclable feedstocks, the development of distributed chemical manufacturing, and the utilization of renewable energy to drive chemical reactions. First, we describe the quantitative impact of these effects on steady-state catalytic turnover rates through a rate expression derived for a generic catalytic reaction (A → B), which illustrates the functional dependence of rates on each category of solvent interaction. Second, we connect these concepts to recent investigations of the effects of solvents on catalysis to show how interactions between solvent and reactant molecules at solid-liquid interfaces influence catalytic reactions. This discussion demonstrates that the design of effective liquid phase catalytic processes benefits from a clear understanding of these intermolecular interactions and their implications for rates and selectivities.

Calcium in Carbonate Water Facilitates the Transport of U(VI) in Brassica juncea Roots and Enables Root-to-Shoot Translocation.

The role of calcium (Ca) on the cellular distribution of U(VI) in Brassica juncea roots and root-to-shoot translocation was investigated using hydroponic experiments, microscopy, and spectroscopy. Uranium accumulated mainly in the roots (727-9376 mg kg-1) after 30 days of exposure to 80 µM dissolved U in water containing 1 mM HCO3 - at different Ca concentrations (0-6 mM) at pH 7.5. However, the concentration of U in the shoots increased 22 times in experiments with 6 mM Ca compared to 0 mM Ca. In the Ca control experiment, transmission electron microscopy-energy-dispersive spectroscopy analyses detected U-P-bearing precipitates in the cortical apoplast of parenchyma cells. In experiments with 0.3 mM Ca, U-P-bearing precipitates were detected in the cortical apoplast and the bordered pits of xylem cells. In experiments with 6 mM Ca, U-P-bearing precipitates aggregated in the xylem with no apoplastic precipitation. These results indicate that Ca in carbonate water inhibits the transport and precipitation of U in the root cortical apoplast and facilitates the symplastic transport and translocation toward shoots. These findings reveal the considerable role of Ca in the presence of carbonate in facilitating the transport of U in plants and present new insights for future assessment and phytoremediation strategies.

Effect of Bicarbonate and Oxidizing Conditions on U(IV) and U(VI) Reactivity in Mineralized Deposits of New Mexico.

We investigated the effect of bicarbonate and oxidizing agents on uranium (U) reactivity and subsequent dissolution of U(IV) and U(VI) mineral phases in the mineralized deposits from Jackpile mine, Laguna Pueblo, New Mexico, by integrating laboratory experiments with spectroscopy, microscopy and diffraction techniques. Uranium concentration in solid samples from mineralized deposit obtained for this study exceeded 7000 mg kg-1, as determined by X-ray fluorescence (XRF). Results from X-ray photoelectron spectroscopy (XPS) suggest the coexistence of U(VI) and U(IV) at a ratio of 19:1 at the near surface region of unreacted solid samples. Analyses made using X-ray diffraction (XRD) and electron microprobe detected the presence of coffinite (USiO4) and uranium-phosphorous-potassium (U-P-K) mineral phases. Imaging, mapping and spectroscopy results from scanning transmission electron microscopy (STEM) indicate that the U-P-K phases were encapsulated by carbon. Despite exposing the solid samples to strong oxidizing conditions, the highest aqueous U concentrations were measured from samples reacted with 100% air saturated 10 mM NaHCO3 solution, at pH 7.5. Analyses using X-ray absorption spectroscopy (XAS) indicate that all the U(IV) in these solid samples were oxidized to U(VI) after reaction with dissolved oxygen and hypochlorite (OCl-) in the presence of bicarbonate (HCO3 -). The reaction between these organic rich deposits, and 100% air saturated bicarbonate solution (containing dissolved oxygen), can result in considerable mobilization of U in water, which has relevance to the U concentrations observed at the Rio Paguate across the Jackpile mine. Results from this investigation provide insights on the reactivity of carbon encapsulated U-phases under mild and strong oxidizing conditions that have important implication in U recovery, remediation and risk exposure assessment of sites.

Effect of Calcium on the Bioavailability of Dissolved Uranium(VI) in Plant Roots under Circumneutral pH.

We integrated field measurements, hydroponic experiments, microscopy, and spectroscopy to investigate the effect of Ca(II) on dissolved U(VI) uptake by plants in 1 mM HCO3- solutions at circumneutral pH. The accumulation of U in plants (3.1-21.3 mg kg-1) from the stream bank of the Rio Paguate, Jackpile Mine, New Mexico served as a motivation for this study. Brassica juncea was the model plant used for the laboratory experiments conducted over a range of U (30-700 µg L-1) and Ca (0-240 mg L-1) concentrations. The initial U uptake followed pseudo-second-order kinetics. The initial U uptake rate ( V0) ranged from 4.4 to 62 µg g-1 h-1 in experiments with no added Ca and from 0.73 to 2.07 µg g-1 h-1 in experiments with 12 mg L-1 Ca. No measurable U uptake over time was detected for experiments with 240 mg L-1 Ca. Ternary Ca-U-CO3 complexes may affect the decrease in U bioavailability observed in this study. Elemental X-ray mapping using scanning transmission electron microscopy-energy-dispersive spectrometry detected U-P-bearing precipitates within root cell walls in water free of Ca. These results suggest that root interactions with Ca and carbonate in solution affect the bioavailability of U in plants. This study contributes relevant information to applications related to U transport and remediation of contaminated sites.

A multi-locus inference of the evolutionary diversification of extant flamingos (Phoenicopteridae).

BACKGROUND: Modern flamingos (Phoenicopteridae) occupy a highly specialized ecology unique among birds and represent a potentially powerful model system for informing the mechanisms by which a lineage of birds adapts and radiates. However, despite a rich fossil record and well-studied feeding morphology, molecular investigations of the evolutionary progression among modern flamingos have been limited. Here, using three mitochondrial (mtDNA) markers, we present the first DNA sequence-based study of population genetic variation in the widely distributed Chilean Flamingo and, using two mtDNA and 10 nuclear (nDNA) markers, recover the species tree and divergence time estimates for the six extant species of flamingos. Phylogenetic analyses include likelihood and Bayesian frameworks and account for potential gene tree discordance. Analyses of divergence times are fossil calibrated at the divergence of Mirandornithes (flamingos + grebes) and the divergence of crown grebes. RESULTS: mtDNA sequences confirmed the presence of a single metapopulation represented by two minimally varying mtDNA barcodes in Chilean flamingos. Likelihood and Bayesian methods recovered identical phylogenies with flamingos falling into shallow-keeled (comprising the Greater, American and Chilean Flamingos) and deep-keeled (comprising the Lesser, Andean and James's Flamingos) sub-clades. The initial divergence among flamingos occurred at or shortly after the Mio-Pliocene boundary (6-3 Ma) followed by quick consecutive divergences throughout the Plio-Pleistocene. There is significant incongruence between the ages recovered by the mtDNA and nDNA datasets, likely due to mutational saturation occurring in the mtDNA loci. CONCLUSION: The finding of a single metapopulation in the widespread Chilean Flamingo confirms similar findings in other widespread flamingo species. The robust species phylogeny is congruent with previous classifications of flamingos based on feeding morphology. Modern phoenicopterids likely originated in the New World with each sub-clade dispersing across the Atlantic at least once. Our divergence time estimates place flamingos among the youngest families of birds, counter to the classical notion of flamingos as among the oldest based on biogeography and the fossil record. Finally, we designate 'Phoeniconaias' as a junior synonym of 'Phoenicoparrus' and redefine the latter genus as containing all flamingos more closely related to Phoenicoparrus andinus than Phoenicopterus roseus.