ABSTRACT
The genus Myzobdella groups five species of leeches parasites of fishes mainly of freshwater but with tolerance to brackish waters. Native distribution of these species includes the New World from North to South America. Myzobdella lugubris Leidy, 1851, the type species of the genus, was briefly described based on specimens from the USA, but subsequently their morphology, known distribution and host range were expanded; however, less is known about the other four species of the genus. As part of a survey focusing on characterizing the diversity of leeches from Mexico, specimens of Myzobdella patzcuarensis (Caballero, 1940), from the type locality of the species were included for the first time in a phylogenetic study. In addition, specimens assigned to Myzobdella from the southeast of Mexico as well as from Nicaragua, were also included. In the resulting phylogenetic tree, our newly generated sequences were found nested in the same clade that M. lugubris; with unresolved relationships and relatively low genetic divergence, suggesting conspecificity. In addition, the internal morphology of the specimens of Myzobdella from Mexico is consistent with the description of M. lugubris. Our morphological examination reveals high degrees of variability in the external pigmentation of the specimens. Based on our results we formally synonymize M. patzcuarensis under M. lugubris.
Subject(s)
Leeches , Phylogeny , Species Specificity , Animals , Leeches/classification , Leeches/anatomy & histology , Leeches/genetics , Leeches/parasitology , MexicoABSTRACT
Leech specimens of the genus Pontobdella (Hirudinida: Piscicolidae) were found off the coast of the state of Oaxaca (Pacific) as well as in Veracruz and Tabasco (Gulf of Mexico), Mexico. Based on the specimens collected in Oaxaca, a redescription of Pontobdella californiana is provided, with emphasis on the differences in the reproductive organs with the original description of the species. In addition, leech cocoons assigned to P. californiana were found attached to items hauled by gillnets and studied using scanning electron microscopy and molecular approaches. Samples of Pontobdella macrothela were found in both Pacific and Atlantic oceans, representing new geographic records. The phylogenetic position of P. californiana is investigated for the first time, and with the addition of Mexican samples of both species, the phylogenetic relationships within Pontobdella are reinvestigated. Parsimony and maximum-likelihood phylogenetic analysis were based on mitochondrial (cytochrome oxidase subunit I [COI] and 12S rRNA) and nuclear (18S rRNA and 28S rRNA) DNA sequences. Based on our results, we confirm the monophyly of Pontobdella and the pantropical distribution of P. macrothela with a new record in the Tropical Eastern Pacific.
Subject(s)
Leeches , Microscopy, Electron, Scanning , Phylogeny , Animals , Leeches/classification , Leeches/genetics , Leeches/anatomy & histology , Mexico , Microscopy, Electron, Scanning/veterinary , Pacific Ocean , Atlantic Ocean , DNA, Ribosomal/chemistry , RNA, Ribosomal, 28S/genetics , Fish Diseases/parasitology , Gulf of Mexico/epidemiology , Electron Transport Complex IV/genetics , Ectoparasitic Infestations/parasitology , Ectoparasitic Infestations/veterinary , RNA, Ribosomal, 18S/genetics , Molecular Sequence Data , Sequence Alignment/veterinary , Likelihood Functions , Fishes/parasitologyABSTRACT
Peruanocotyle pelagica n. sp. is described based on specimens collected from the wall of the pharyngeal cavity of the Pacific cownose ray Rhinoptera steindachneri offshore Oaxaca and Guerrero, Mexico. The new species is distinguished from Peruanocotyle chisholmae by its anchors, which include a slender guard that curves towards the tip of the blade and which lack an accessory piece, morphological differences of the seminal vesicle, the lack of a male copulatory organ accessory piece and a greater number of spines, and an unsclerotized vagina. Molecular data of Peruanocotyle pelagica were generated to place the phylogenetic position of the genus within Monocotylidae.
Subject(s)
Acanthocephala , Elasmobranchii , Parasites , Skates, Fish , Trematoda , Animals , Female , Male , Mexico/epidemiology , Pacific Ocean , Phylogeny , Skates, Fish/parasitologyABSTRACT
A new genus and species of Hexabothriidae Price, 1942 is described from specimens infecting the gill of the numbfish Narcine entemedor Jordan and Starks from the Pacific coast of Mexico. In addition to the new taxon described here, species of 8 genera also display symmetrical haptors and have eggs with two polar filaments. However, they differ in the combination of the following features: distal portion of the male copulatory organ tubular, unarmed and proximally dilated confining an internal coiled duct, as well as dorsal origin of haptoral appendix. Based on molecular data derived from 3 loci, the mitochondrial Cytochrome C oxidase subunit 1 as well as the nuclear ribosomal 18S and 28S, the new species was found nested within Hexabothriidae together with the other 4 genera with representatives in GenBank. This is the first species of Hexabothriidae reported from a species of Narcinidae.
