A high throughput instrument to measure mechanical losses in thin film coatings.

Brownian thermal noise generated by mechanical losses in thin film coatings limits the sensitivity of gravitational wave detectors, as well as several high precision metrology experiments. Improving the sensitivity of the next generation of gravitational wave detectors will require optical coatings with significantly reduced mechanical losses. In this paper, we describe a system that we developed to measure the mechanical loss angle of thin film coatings deposited on fused silica substrates. The novelty of this system resides in the capability of parallel measurement of up to four samples and the ability to simultaneously probe all the resonant modes of each sample. This high throughput measurement system allows the exploration of a large number of deposition and material parameters, which can be tuned to achieve low loss coatings.

Design of a tuned mass damper for high quality factor suspension modes in Advanced LIGO.

We discuss the requirements, design, and performance of a tuned mass damper which we have developed to damp the highest frequency pendulum modes of the quadruple suspensions which support the test masses in the two advanced detectors of the Laser Interferometric Gravitational-Wave Observatory. The design has to meet the requirements on mass, size, and level of damping to avoid unduly compromising the suspension thermal noise performance and to allow retrofitting of the dampers to the suspensions with minimal changes to the existing suspensions. We have produced a design satisfying our requirements which can reduce the quality factor of these modes from ∼500 000 to less than 10 000, reducing the time taken for the modes to damp down from several hours to a few minutes or less.

Iron chelators reduce chromosomal breaks in ataxia-telangiectasia cells.

Ataxia-telangiectasia (A-T) is characterized by ataxia, genomic instability, and increased cancer incidence. Previously, iron chelator concentrations which suppressed normal cell colony formation increased A-T cell colony formation. Similarly, iron chelators preferentially increased A-T cell colony formation following peroxide exposure compared to normal cells. Last, A-T cells exhibited increased short-term sensitivity to labile iron exposure compared to normal cells, an event corrected by recombinant ATM (rATM) expression. Since chromosomal damage is important in A-T pathology and iron chelators exert beneficial effects on A-T cells, we hypothesized that iron chelators would reduce A-T cell chromosomal breaks. We treated A-T, normal, and A-T cells expressing rATM with labile iron, iron chelators, antioxidants, and t-butyl hydroperoxide, and examined chromosomal breaks and ATM activation. Additionally, the effect of ATM-deficiency on transferrin receptor (TfR) expression and TfR activity blockage in A-T and syngeneic A-T cells expressing rATM was examined. We report that (1) iron chelators and iron-free media reduce spontaneous and t-butyl hydroperoxide-induced chromosomal breaks in A-T, but not normal, or A-T cells expressing rATM; (2) labile iron exposure induces A-T cell chromosomal breaks, an event lessened with rATM expression; (3) desferal, labile iron, and copper activate ATM; (4) A-T cell TfR expression is lowered with rATM expression and (5) blocking TfR activity with anti-TfR antibodies increases A-T cell colony formation, while lowering chromosomal breaks. ATM therefore functions in iron responses and the maintenance of genomic stability following labile iron exposure.

Action of sympathetic nerves of inner and outer muscle of sheep carotid artery, and effect of pressure on nerve distribution.

1. The direction of torsion produced during active shortening of helical strips of sheep carotid arteries was measured to assess whether inner or outer muscle was contracting. 2. Noradrenaline contracted inner (non-innervated) muscle in lower concentrations than were needed to contract outer (innervated) muscle, even with desipramine present to prevent uptake of noradrenaline by the nerves and with enough cyanide present to rise the normally low O2 tension of inner muscle to that of outer muscle. 3. Activation of sympathetic nerves in the outer part of the artery by nicotine caused almost evenly balanced contraction of both parts of the wall, with slight bias to outer contraction. 4. Moderate external constriction of the artery in vivo for 10-17 days, in order to raise pressure throughout the wall to intraluminal pressure, made the entire wall nerve-free. 5. The results provide evidence that the nerves can induce substantial activation of inner muscle, which is highly sensitive to noradrenaline, and that the absence of nerves from inner muscle can be explained by the high pressure there.