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1.
Carcinogenesis ; 34(12): 2880-90, 2013 Dec.
Article in English | MEDLINE | ID: mdl-23825155

ABSTRACT

Elevated expression of p130Cas (Crk-associated substrate)/BCAR1 (breast cancer antiestrogen resistance 1) in human breast tumors is a marker of poor prognosis and poor overall survival. p130Cas is a downstream target of the tyrosine kinase c-Src. Signaling mediated by p130Cas through its phosphorylated substrate domain (SD) and interaction with effector molecules directly promotes tumor progression. We previously developed a constitutively phosphorylated p130Cas SD molecule, Src*/SD (formerly referred to as Src*/CasSD), which acts as decoy molecule and attenuates the transformed phenotype in v-crk-transformed murine fibroblasts and human breast cancer cells. To test the function of this molecule in vivo, we established mouse mammary tumor virus (MMTV)-long terminal repeat-Src*/SD transgenic mice in which mammary gland development and tumor formation were analyzed. Transgenic expression of the Src*/SD molecule under the MMTV-long terminal repeat promoter did not interfere with normal mammary gland development or induce tumors in mice observed for up to 11 months. To evaluate the effects of the Src*/SD molecule on tumor development in vivo, we utilized the MMTV-polyoma middle T-antigen (PyMT) murine breast cancer model that depends on c-Src. PyMT mice crossed with Src*/SD mice displayed accelerated tumor formation. The earlier onset of tumors can be explained by the interaction of the Src* domain with PyMT and targeting the fused phosphorylated SD to the membrane. At membrane compartments, it might integrate membrane-associated active signaling complexes leading to increased proliferation measured by phospho-Histone H3 staining. Although these results were unexpected, they emphasize the importance of preventing the membrane association of Src*/SD when employed as decoy molecule.


Subject(s)
Breast Neoplasms/genetics , Breast Neoplasms/pathology , Crk-Associated Substrate Protein/genetics , Genes, src/genetics , Phosphorylation/genetics , Animals , Breast Neoplasms/metabolism , Carcinogenesis/genetics , Carcinogenesis/pathology , Crk-Associated Substrate Protein/metabolism , Disease Progression , Female , Fibroblasts/metabolism , Fibroblasts/pathology , Mammary Glands, Animal/metabolism , Mammary Glands, Animal/pathology , Mammary Neoplasms, Experimental/genetics , Mammary Neoplasms, Experimental/metabolism , Mammary Neoplasms, Experimental/pathology , Mammary Tumor Virus, Mouse/genetics , Mammary Tumor Virus, Mouse/metabolism , Mice , Mice, Transgenic/genetics , Mice, Transgenic/metabolism , Rats
2.
Mol Vis ; 15: 685-99, 2009.
Article in English | MEDLINE | ID: mdl-19365570

ABSTRACT

PURPOSE: AND-34/BCAR3 (Breast Cancer Anti-Estrogen Resistance 3) associates with the focal adhesion adaptor protein, p130CAS/BCAR1. Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. We sought to establish the effects of the loss of AND-34 expression in a mammalian organism. METHODS: AND-34(-/-) mice were generated by homologous recombination. Histopathology, in situ hybridization, and western blotting were performed on murine tissues. RESULTS: Western analyses confirmed total loss of expression in AND-34(-/-) splenic lymphocytes. Mice lacking AND-34 are fertile and have normal longevity. While AND-34 is widely expressed in wild type mice, histologic analysis of multiple organs in AND-34(-/-) mice is unremarkable and analyses of lymphocyte development show no overt changes. A small percentage of AND-34(-/-) mice show distinctive small white eye lesions resulting from the migration of ruptured cortical lens tissue into the anterior chamber. Following initial vacuolization and liquefaction of the lens cortex first observed at postnatal day three, posterior lens rupture occurs in all AND-34(-/-) mice, beginning as early as three weeks and seen in all mice at three months. Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. Prior data link AND-34 expression to the activation of Akt signaling. While Akt Ser 473 phosphorylation was readily detectable in AND-34(+/+) lens epithelial cells, it was markedly reduced in the AND-34(-/-) lens epithelium. Basal levels of p130Cas phosphorylation were higher in AND-34(+/+) than in AND-34(-/-) lens epithelium. CONCLUSIONS: These results demonstrate the loss of AND-34 dysregulates focal adhesion complex signaling in lens epithelial cells and suggest that AND-34-mediated signaling is required for maintenance of the structural integrity of the adult ocular lens.


Subject(s)
Guanine Nucleotide Exchange Factors/deficiency , Lens, Crystalline/metabolism , Lens, Crystalline/pathology , Adaptor Proteins, Signal Transducing , Animals , Animals, Newborn , Crk-Associated Substrate Protein/metabolism , Epithelium/metabolism , Epithelium/pathology , Exons/genetics , Gene Expression Profiling , Gene Expression Regulation, Developmental , Gene Targeting , Guanine Nucleotide Exchange Factors/genetics , Guanine Nucleotide Exchange Factors/metabolism , Mice , Organ Specificity , Paraffin Embedding , Phenotype , Phosphorylation , Proto-Oncogene Proteins c-akt/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rupture, Spontaneous/pathology , Serine/metabolism , Signal Transduction
3.
J Cell Sci ; 120(Pt 14): 2366-77, 2007 Jul 15.
Article in English | MEDLINE | ID: mdl-17606992

ABSTRACT

Members of the CMS/CIN85 protein family participate in clathrin-mediated endocytosis and play a crucial role in maintaining the kidney filtration barrier. The CMS protein structure includes three Src homology 3 (SH3) domains and a proline-rich (PR) region that is connected by a 'linker' sequence to a coiled-coil (CC) domain. We show that CMS is a component of special actin-rich adhesion structures--podosomes--and demonstrate specific actin-binding properties of CMS. We have found that the entire C-terminal half of CMS is necessary for efficient binding to filamentous actin (F-actin). CMS and CIN85 can crosslink F-actin into bundles, a function that depends on the PR region and the CC domain. Removal of these domains reduces migration. CMS can also form heterotypic complexes with CIN85. CIN85 is expressed as multiple isoforms that share the CC domain, suggesting that heterotypic interactions with CMS provides a mechanism to regulate CMS binding to F-actin and thus for modulating dynamic rearrangements of the cytoskeleton.


Subject(s)
Actins/metabolism , Adaptor Proteins, Signal Transducing/metabolism , Cytoskeletal Proteins/metabolism , Podocytes/metabolism , Actins/isolation & purification , Adaptor Proteins, Signal Transducing/genetics , Adaptor Proteins, Signal Transducing/isolation & purification , Amino Acid Sequence , Animals , Cell Line , Cell Movement , Cytoskeleton/metabolism , Humans , Mice , Molecular Sequence Data , NIH 3T3 Cells , Podocytes/cytology , src Homology Domains/genetics , src Homology Domains/physiology
4.
Matrix Biol ; 26(6): 442-55, 2007 Jul.
Article in English | MEDLINE | ID: mdl-17482450

ABSTRACT

Within the nervous system, heparan sulfate (HS) of the cell surface and extracellular matrix influences developmental, physiologic and pathologic processes. HS is a functionally diverse polysaccharide that employs motifs of sulfate groups to selectively bind and modulate various effector proteins. Specific HS activities are modulated by 3-O-sulfated glucosamine residues, which are generated by a family of seven 3-O-sulfotransferases (3-OSTs). Most isoforms we herein designate as gD-type 3-OSTs because they generate HS(gD+), 3-O-sulfated motifs that bind the gD envelope protein of herpes simplex virus 1 (HSV-1) and thereby mediate viral cellular entry. Certain gD-type isoforms are anticipated to modulate neurobiologic events because a Drosophila gD-type 3-OST is essential for a conserved neurogenic signaling pathway regulated by Notch. Information about 3-OST isoforms expressed in the nervous system of mammals is incomplete. Here, we identify the 3-OST isoforms having properties compatible with their participation in neurobiologic events. We show that 3-OST-2 and 3-OST-4 are principal isoforms of brain. We find these are gD-type enzymes, as they produce products similar to a prototypical gD-type isoform, and they can modify HS to generate receptors for HSV-1 entry into cells. Therefore, 3-OST-2 and 3-OST-4 catalyze modifications similar or identical to those made by the Drosophila gD-type 3-OST that has a role in regulating Notch signaling. We also find that 3-OST-2 and 3-OST-4 are the predominant isoforms expressed in neurons of the trigeminal ganglion, and 3-OST-2/4-type 3-O-sulfated residues occur in this ganglion and in select brain regions. Thus, 3-OST-2 and 3-OST-4 are the major neural gD-type 3-OSTs, and so are prime candidates for participating in HS-dependent neurobiologic events.


Subject(s)
Central Nervous System/enzymology , Peripheral Nervous System/enzymology , Sulfotransferases/metabolism , Amino Acid Sequence , Animals , Base Sequence , Brain/enzymology , Brain/metabolism , CHO Cells , Central Nervous System/cytology , Central Nervous System/metabolism , Cricetinae , Cricetulus , Female , Gene Expression Regulation, Enzymologic , Heparitin Sulfate/metabolism , Herpesvirus 1, Human/physiology , Humans , In Situ Hybridization , Isoenzymes/genetics , Isoenzymes/metabolism , Male , Mice , Mice, Inbred C57BL , Molecular Sequence Data , Neurons/enzymology , Neurons/metabolism , Peripheral Nervous System/cytology , Peripheral Nervous System/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Substrate Specificity , Sulfotransferases/genetics , Virus Internalization
5.
Am J Physiol Lung Cell Mol Physiol ; 291(2): L232-43, 2006 Aug.
Article in English | MEDLINE | ID: mdl-16473861

ABSTRACT

Neutrophil elastase (NE) plays an important role in emphysema, a pulmonary disease associated with excessive elastolysis and ineffective repair of interstitial elastin. Besides its direct elastolytic activity, NE releases soluble epidermal growth factor receptor (EGFR) ligands and initiates EGFR/MEK/ERK signaling to downregulate tropoelastin mRNA in neonatal rat lung fibroblasts (DiCamillo SJ, Carreras I, Panchenko MV, Stone PJ, Nugent MA, Foster JA, and Panchenko MP. J Biol Chem 277: 18938-18946, 2002). We now report that NE downregulates tropoelastin mRNA in the rat fetal lung fibroblast line RFL-6. The tropoelastin mRNA downregulation is preceded by release of EGF-like and TGF-alpha-like polypeptides and requires EGFR/MEK/ERK signaling, because it is prevented by the EGFR inhibitor AG1478 and the MEK/ERK uncoupler U0126. Tropoelastin expression in RFL-6 fibroblasts is governed by autocrine TGF-beta signaling, because TGF-beta type I receptor kinase inhibitor or TGF-beta neutralizing antibody dramatically decreases tropoelastin mRNA and protein levels. Half-life of tropoelastin mRNA in RFL-6 cells is >24 h, but it is decreased to approximately 8 h by addition of TGF-beta neutralizing antibody, EGF, TGF-alpha, or NE. Tropoelastin mRNA destabilization by NE, EGF, or TGF-alpha is abolished by AG1478 or U0126. EGF-dependent tropoelastin mRNA downregulation is reversed upon ligand withdrawal, whereas chronic EGF treatment leads to persistent downregulation of tropoelastin mRNA and protein levels and decreases insoluble elastin deposition. We conclude that NE-initiated EGFR/MEK/ERK signaling cascade overrides the autocrine TGF-beta signaling on tropoelastin mRNA stability and, therefore, decreases the elastogenic response in RFL-6 fibroblasts. We hypothesize that persistent EGFR/MEK/ERK signaling could impede the TGF-beta-induced elastogenesis/elastin repair in the chronically inflamed, elastase/anti-elastase imbalanced lung in emphysema.


Subject(s)
ErbB Receptors/metabolism , Extracellular Signal-Regulated MAP Kinases/metabolism , Fibroblasts/physiology , Leukocyte Elastase/metabolism , MAP Kinase Kinase 1/metabolism , Signal Transduction/physiology , Transforming Growth Factor beta/metabolism , Tropoelastin/metabolism , Animals , Autocrine Communication , Cell Line , Dichlororibofuranosylbenzimidazole/metabolism , Enzyme Inhibitors/metabolism , Fibroblasts/cytology , Fibroblasts/ultrastructure , Gene Expression Regulation , Humans , Lung/cytology , Mice , Nucleic Acid Synthesis Inhibitors/metabolism , RNA Stability , RNA, Messenger/metabolism , Rats , Tropoelastin/genetics
6.
J Cell Biochem ; 92(5): 1034-43, 2004 Aug 01.
Article in English | MEDLINE | ID: mdl-15258925

ABSTRACT

Atherosclerosis is a multifactorial disease, the progression of which is modulated by several factors, including inflammation and hypercholesterolemia. The A(3) adenosine receptor (A(3)AR) has been reported to affect mast cell degranulation leading to inflammation, as well as to influence cardiovascular homeostasis. Here, we show that its deletion can also impact vascular smooth muscle cell (VSMC) proliferation in vitro. Based on these observations, we hypothesized that A(3)AR deficiency would affect atheromatous lesion development in vivo. Our results indicate that the expression of the matrix enzyme lysyl oxidase (LO) is increased while the proliferation potential of VSMC is decreased in A(3)AR-null aortas. This is in accordance with the previously reported inverse correlation between LO level and proliferation. Nevertheless, we found that A(3)-deficiency does not protect vessels against atherogenesis. This was demonstrated in mouse models of high fat diet-induced atherosclerosis and guidewire-induced femoral artery injury. We conclude that the contributions of the A(3)AR to inflammation and to modulating LO levels are not significant enough to control vascular response to injury.


Subject(s)
Arteriosclerosis/genetics , Receptor, Adenosine A3/physiology , Animals , Aorta/cytology , Aorta/enzymology , Aorta/metabolism , Base Sequence , Blotting, Western , Cells, Cultured , DNA Primers , DNA Replication , Elastin/metabolism , Immunohistochemistry , Mice , Mice, Inbred C57BL , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/enzymology , Muscle, Smooth, Vascular/metabolism , Protein-Lysine 6-Oxidase/metabolism , Receptor, Adenosine A3/genetics , Up-Regulation
7.
FEBS Lett ; 532(3): 267-72, 2002 Dec 18.
Article in English | MEDLINE | ID: mdl-12482577

ABSTRACT

Sites of A3 adenosine receptor gene expression have not been fully explored nor has this gene's promoter activity been confirmed in vivo. Transgenic mice were generated in which 2.3 kb upstream of the transcriptional start site of the mouse A3 adenosine receptor was coupled to a beta-galactosidase reporter gene. Selective transgene expression was detected in testis and brain as well as at other sites in which A3 adenosine receptor message has not been previously reported, including retinal ganglion cells and smooth muscle cells of the cerebrospinal vasculature. Our study suggests that this promoter may be useful in the selective targeting of gene expression to specific tissues.


Subject(s)
Promoter Regions, Genetic , Receptors, Purinergic P1/genetics , Animals , Brain/metabolism , Female , Gene Expression Regulation , In Situ Hybridization , Lac Operon , Male , Mice , Mice, Transgenic , Models, Genetic , Plasmids/metabolism , Protein Binding , Protein Structure, Tertiary , Rats , Rats, Sprague-Dawley , Receptor, Adenosine A3 , Retina/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Testis/metabolism , Transgenes , beta-Galactosidase/metabolism
8.
Blood ; 100(5): 1670-8, 2002 Sep 01.
Article in English | MEDLINE | ID: mdl-12176887

ABSTRACT

Fragmentation of polyploid megakaryocytes into platelets has great relevance for blood homeostasis. Apoptotic cell death is a highly regulated genetic program, which has been observed in mature megakaryocytes fragmenting into platelets. The antiapoptotic protein BclxL has been reported as up-regulated during megakaryocytic differentiation in vitro, but absent during late megakaryopoiesis. Our study focused on examining BclxL levels in megakaryocytes in vivo and in assessing the effect of its overexpression in transgenic mice (via the platelet factor 4 [PF4] promoter) on megakaryocyte development and platelet fragmentation. Interestingly, in the wild-type and less in PF4-driven transgenic mice, BclxL was not detected in a fraction of the large mature megakaryocytes, suggesting a regulation on the protein level. BclxL overexpression was associated with a moderate increase in megakaryocyte number, with no significant change in ploidy level or platelet counts. When the mice were challenged by induction of immune thrombocytopenia, the rate of platelet recovery was significantly slower in the transgenic mice as compared with controls. Moreover, proplatelet formation in vitro by transgenic megakaryocytes was limited. Transgenic megakaryocytes displayed poorly developed platelet demarcation membranes and cell margin extensions. Our study indicates that regulated expression of BclxL in megakaryocytes is important for the development of cells with a high potential to fragment into platelets.


Subject(s)
Blood Platelets/cytology , Cell Differentiation/genetics , Gene Expression Regulation , Megakaryocytes/cytology , Megakaryocytes/physiology , Proto-Oncogene Proteins c-bcl-2/genetics , Animals , Apoptosis/genetics , Blood Platelets/physiology , Mice , Mice, Transgenic , Thrombocytopenia/genetics , Thrombocytopenia/immunology , bcl-X Protein
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