Vitality as a measure of animal welfare during purse seine pumping related crowding of Atlantic mackerel (Scomber scrombrus).

The impacts of wild capture fishing on animal welfare are poorly understood. During purse seine fishing for Atlantic mackerel (Scomber scrombrus), catches are crowded to high densities to facilitate pumping onboard. This study aimed to monitor fish welfare during crowding events in the Norwegian purse seine fishery, and to identify relevant drivers. We first correlated a suite of neuro-endocrine, physiological and physical stress responses (integrated into a single measure of welfare using multivariate analysis) to the behavioural vitality of individual mackerel in controlled crowding trials in aquaculture cages. Vitality was found to be a useful measure of welfare. We then assessed individual fish vitality onboard a commercial purse seiner. Catch welfare, measured using vitality, was observed to be negatively impacted during pumping related crowding. Larger catches and longer crowding exposure times resulted in greater negative impacts. Vitality was not significantly impacted by crowding density or dissolved oxygen concentrations inside the net, although methodological limitations limited accurate measurement of these parameters. Blood lactate levels correlated negatively with vitality, suggesting that high-intensity anaerobic locomotory activity was associated with the reduction in welfare. Based on these findings, catch welfare could be improved by targeting smaller schools to minimise crowding exposure times.

Effects on individual level behaviour in mackerel (Scomber scombrus) of sub-lethal capture related stressors: Crowding and hypoxia.

Stress to fish during harvest in wild capture fisheries is known to negatively influence subsequent survival in catches that are released. Therefore, if fisheries are to be conducted sustainably, there is a need to promote good fish welfare during the capture process. Purse seine fishing is a widespread and efficient fishing method. However, capture and release of fish from purse seines (a process called "slipping") can result in extremely high mortality in small pelagic schooling species. The objective of this study was to establish behavioural indicators of sub-lethal stress in Atlantic mackerel (Scomber scombrus) that may be used to set safe threshold limits for use in commercial purse seine fishing, in order to ensure good fish welfare and thereby minimise slipping mortality. Controlled mesocosm scale experiments with schools of mackerel in net pens were undertaken to determine behavioural responses to simulated purse seine capture stressors of "crowding", "hypoxia" and "crowding & hypoxia". Crowding (at 30 kg.m-3) was achieved by reducing the volume of the net pen, while hypoxia (to 40% oxygen saturation) was achieved by surrounding the net pen with a tarpaulin bag to prevent water exchange. Using video analysis, we investigated behavioural responses in nearest neighbour distances, nearest neighbour angular deviations, tail beat amplitude and tail beat frequency (TBF). Of the metrics considered, only TBF showed a response; a significant increase to "crowding" (42% increase) and "crowding & hypoxia" (38% increase) was found. The increase in TBF in response to "hypoxia" alone (29% increase) was not significant. We therefore conclude that increases in tail beat frequency may be used as an indicator of sub-lethal purse seine capture stress in mackerel that may have utility in minimising post slipping mortality.

Behavioural and welfare implications of a new slipping methodology for purse seine fisheries in Norwegian waters.

The release of unwanted fish from purse seines whilst still in the water is termed slipping and may lead to significant mortality following release. The objective of this study was to determine the fish welfare implications of a new slipping methodology in which fish are released via a discharge opening formed in the bunt end of the purse seine net. Video analyses of collective and individual level fish behaviour were undertaken in the Norwegian mackerel and herring purse seine fisheries, to quantitively describe slipping behaviour and to determine its driving factors. The majority of fish escaped the purse seine with the schooling structure intact as part of large groups towards the end of slipping process, increasing their speed following escape. However, there was also a tendency (24% of all escapes) to escape in a manner likely to impact negatively upon their welfare, with a breakdown in schooling structure and physical contact with the fishing gear and conspecifics. The tendency to express such welfare compromising behaviour was higher for mackerel than for herring, but was also influenced by the vessel releasing the fish, the amount of fish being slipped, how long the discharge opening had been open and the particular slipping event. These results provide important information for future science-based development of welfare friendly slipping practises.