Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Mutagenesis ; 34(4): 331-340, 2019 12 19.
Article in English | MEDLINE | ID: mdl-31291449

ABSTRACT

Mutations in oncogenes or tumour suppressor genes cause increases in cell growth capacity. In some cases, fully malignant cancer cells develop after additional mutations occur in initially mutated cells. In such instances, the risk of cancer would increase in response to growth of these initially mutated cells. To ascertain whether such a situation might occur in cultured cells, three independent cultures of human lymphoblastoid GM00130 cells were treated with N-ethyl-N-nitrosourea to induce mutations, and the cells were maintained for 12 weeks. Mutant frequencies and spectra of the cells at the MspI and HaeIII restriction sites located at codons 247-250 of the TP53 gene were examined. Mutant frequencies at both sites in the gene exhibited a declining trend during cell culture and reached background levels after 12 weeks; this was also supported by mutation spectra findings. These results indicate that the mutations detected under our assay conditions are disadvantageous to cell growth.


Subject(s)
Ethylnitrosourea/adverse effects , Genes, p53 , Mutation Rate , Mutation/drug effects , Cell Line, Tumor , Dose-Response Relationship, Drug , Humans , Reproducibility of Results , Sequence Analysis, DNA
SELECTION OF CITATIONS
SEARCH DETAIL
...