ABSTRACT
This study is about enrichment of hemopoietic stem cell concentration in mouse bone marrow by use of a glass wool filtration technique. Maximum stem cell concentration was accomplished when filtration conditions were as follows: (1) support medium supplemented to 15% with fresh serum from syngeneic donors; (2) no incubation time allowed for cells on the columns; and (3) an operational temperature of 24 degrees C. With these conditions, stem cell concentrations were increased 5,4-fold relative to the concentration in untreated marrow.
Subject(s)
Filtration/methods , Hematopoietic Stem Cells , Animals , Cell Division , Cells, Cultured , Clone Cells , Mice , Specimen HandlingABSTRACT
The effects of specific dead tumor cell immunization and nonspecific immunostimulation with Corynebacterium parvum on the s.c. growth of the line 1 carcinoma in syngeneic BALB/c mice have been studied. Injection of heavily irradiated line ) carcinoma cells did not inhibit the transplantability or growth of the line 1 carcinoma, and in certain cases these treatments actually prolonged the period of rapid growth. This latter observation was traced to a mild inhibition of metastatic spread, which itself can slow the s.c. tumor growth. Treatment of the mice with 0.25 mg of C. parvum 7 days prior to transplant of the tumor had no effect on its growth by itself; but in combination with i.v.-injected tumor cells, which themselves had no effect on tumor growth, a 45% inhibition of tumor growth was induced. These data demonstrate that, in the weekly immunogenic line 1 carcinoma system, inhibition of s.c. transplants requires not only exposure to tumor antigens but also stimulation of the immunological reactivity.
Subject(s)
Cell Line , Immunization , Lung Neoplasms/immunology , Neoplasm Metastasis , Transplantation Immunology , Animals , Female , Injections, Intravenous , Injections, Subcutaneous , Male , Mice , Mice, Inbred BALB C , Neoplasm Transplantation , Neoplasms, Experimental/immunology , Propionibacterium acnes/immunology , Transplantation, HomologousSubject(s)
Adenocarcinoma, Bronchiolo-Alveolar , Cells, Cultured/drug effects , Lung Neoplasms , Neuraminidase/toxicity , Adenocarcinoma, Bronchiolo-Alveolar/immunology , Animals , Antigens, Neoplasm , Cell Division , Cell Survival/drug effects , Cells, Cultured/metabolism , Dose-Response Relationship, Drug , Hot Temperature , Lung Neoplasms/immunology , Methods , Mice , Mice, Inbred BALB C , Neoplasm Transplantation , Thymidine/metabolism , Transplantation, Homologous , Tritium , Trypan Blue , Trypsin/pharmacology , Vibrio cholerae/enzymologySubject(s)
Adenocarcinoma, Bronchiolo-Alveolar/immunology , Immunosuppression Therapy , Lung Neoplasms/immunology , Neoplasm Transplantation , Aging , Animals , Antibodies, Neoplasm/analysis , Body Weight , Graft Rejection , Hemolytic Plaque Technique , Hydrocortisone/pharmacology , Immunity, Cellular , Lymph Nodes/immunology , Male , Mice , Mice, Inbred BALB C , Neoplasm Metastasis , Neoplasms, Experimental/immunology , Radiation Effects , Spleen/immunology , Splenectomy , Transplantation, HomologousSubject(s)
Drinking , Mice/physiology , Water , Animals , Half-Life , Housing, Animal , Male , Mathematics , Methods , Radioisotopes , RubidiumSubject(s)
Graft vs Host Disease/metabolism , Lipid Metabolism , Serine/metabolism , Animals , Body Weight , Carbon Dioxide/metabolism , Chromatography, Thin Layer , Formates/metabolism , Graft vs Host Reaction , Liver/metabolism , Male , Mice , Oxygen Consumption , Phosphatidylethanolamines/metabolism , Phosphatidylinositols/metabolism , Phospholipids/metabolism , Radiation Chimera , Spleen/transplantation , Transplantation, Homologous , Triglycerides/metabolismSubject(s)
Antibody Formation , Bone Marrow Transplantation , Radiation Chimera , Transplantation Immunology , Animals , Antibody-Producing Cells , Antigens, Bacterial , Body Weight , Erythrocytes/immunology , Graft vs Host Disease , Graft vs Host Reaction , Immunoglobulin G , Immunoglobulin M , Mice , Radiation Injuries, Experimental/surgery , Rats , Salmonella typhi , Sheep , Transplantation, HomologousABSTRACT
Two statistical experimental designs were used to investigate factors involved in 90-day mortality from secondary disease in lethally irradiated mice treated with rat bone marrow. Secondary disease is a graft-versus-host syndrome that has a mortality of about 65-95% in this transplant situation. When the factors--age-of-donor-cells, day-of-cell-injection, dose-of-marrow-cells, sex, and environment--were examined for their main effects and interactions, some combinations of these factors were found to give about 25% 90-day mortality. The experiments indicate that the lowest mortality can be achieved with an injection of 40 million or more cells 1 day after irradiation and an ultraclean environment of unlimited filter-top caging.
Subject(s)
Bone Marrow Transplantation , Environment, Controlled , Graft vs Host Disease/mortality , Models, Statistical , Radiation Chimera , Whole-Body Irradiation , Age Factors , Animals , Female , Male , Mice , Rats , Sex Factors , Time Factors , Whole-Body Irradiation/mortalitySubject(s)
Neoplasms, Experimental/chemically induced , Nitrosamines/administration & dosage , Adenoma/chemically induced , Animals , Hemangiosarcoma/chemically induced , Leukemia, Experimental/chemically induced , Leukemia, Experimental/mortality , Liver Neoplasms/chemically induced , Lung Neoplasms/chemically induced , Male , MiceSubject(s)
Neoplasms/chemically induced , Nitrosamines/pharmacology , Age Factors , Animals , Male , Mice , Nitrosamines/adverse effects , Time FactorsABSTRACT
The incidences of lung tumors and thymic lymphomas were increased in young adult male RF mice receiving 30 milligrams of methyl meth anesulfonate per kilogram of body weight daily in the drinking water throughout life. Differences in oncogenicity between treatment with methyl methanesulfonate and with dimethylnitrosamine or diethylnitrosamine suggest a qualitative difference between the site (or sites) of alkylation by methyl methanesulfonate and by dimethylnitrosamine or diethylnitrosamine within the nucleic acids.
