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1.
Chem Senses ; 39(5): 403-14, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24682237

ABSTRACT

It has been reported that a subset of type III taste cells express glutamate decarboxylase (GAD)67, which is a molecule that synthesizes gamma-aminobutyric acid (GABA), and that Mash1 could be a potential regulator of the development of GABAnergic neurons via Dlx transcription factors in the central nervous system. In this study, we investigated the expression of GAD67 and Dlx in the embryonic taste buds of the soft palate and circumvallate papilla using Mash1 knockout (KO)/GAD67-GFP knock-in mice. In the wild-type animal, a subset of type III taste cells contained GAD67 in the taste buds of the soft palate and the developing circumvallate papilla, whereas GAD67-expressing taste bud cells were missing from Mash1 KO mice. A subset of type III cells expressed mRNA for Dlx5 in the wild-type animals, whereas Dlx5-expressing cells were not evident in the apical part of the circumvallate papilla and taste buds in the soft palate of Mash1 KO mice. Our results suggest that Mash1 is required for the expression of GAD67 and Dlx5 in taste bud cells.


Subject(s)
Basic Helix-Loop-Helix Transcription Factors/genetics , Glutamate Decarboxylase/metabolism , Homeodomain Proteins/metabolism , Taste Buds/metabolism , Animals , Female , Gene Expression Regulation, Developmental , Glutamate Decarboxylase/genetics , Homeodomain Proteins/genetics , Mice, Knockout , Palate, Soft/embryology , Palate, Soft/metabolism , Tongue/embryology , Tongue/metabolism
2.
Cell Tissue Res ; 353(3): 391-8, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23636420

ABSTRACT

Synaptogyrins are conserved components of the exocytic apparatus and function as regulators of Ca(2+)-dependent exocytosis. The synaptogyrin family comprises three isoforms: two neuronal (synaptogyrin-1 and -3) and one ubiquitous (synaptogyrin-2) form. Although the expression patterns of the exocytic proteins synaptotagmin-1, SNAP-25, synaptobrevin-2 and synaptophysin have been elucidated in taste buds, the function and expression pattern of synaptogyrin-1 in rat gustatory tissues have not been determined. Therefore, we examined the expression patterns of synaptogyrin-1 and several cell-specific markers of type II and III cells in rat gustatory tissues. Reverse transcription/polymerase chain reaction assays and immunoblot analysis revealed the expression of synaptogyrin-1 mRNA and its protein in circumvallate papillae. In fungiform, foliate and circumvallate papillae, the antibody against synaptogyrin-1 immunolabeled a subset of taste bud cells and intra- and subgemmal nerve processes. Double-labeling experiments revealed the expression of synaptogyrin-1 in most taste cells immunoreactive for aromatic L-amino acid decarboxylase and the neural cell adhesion molecule. A subset of synaptogyrin-1-immunoreactive taste cells also expressed phospholipase Cß2, gustducin, or sweet taste receptor (T1R2). In addition, most synaptogyrin-1-immunoreactive taste cells expressed synaptobrevin-2. These results suggest that synaptogyrin-1 plays a regulatory role in transmission at the synapses of type III cells and is involved in exocytic function with synaptobrevin-2 in a subset of type II cells in rat taste buds.


Subject(s)
Gene Expression Regulation/physiology , Receptors, G-Protein-Coupled/biosynthesis , Synaptogyrins/biosynthesis , Taste Buds/metabolism , Animals , Exocytosis/physiology , Male , RNA, Messenger/biosynthesis , Rats , Rats, Sprague-Dawley , Signal Transduction/physiology , Taste Buds/cytology , Vesicle-Associated Membrane Protein 2/biosynthesis
3.
Arch Gerontol Geriatr ; 54(1): 28-33, 2012.
Article in English | MEDLINE | ID: mdl-21592601

ABSTRACT

Although poor physical fitness is known to be associated with increased mortality in adult and elderly populations, this association is not conclusive in very elderly. The purpose of the present study was to evaluate the association for a very old community-dwelling population. The participants (90 males, 117 females) were 85-year-old individuals residing in Fukuoka, Japan. Baseline examinations including muscle strength of the handgrip and leg extension, one-leg standing, leg stepping rate, and walking were performed in 2003 and these subjects were followed for 6.5 years. During the follow-up period, 81 individuals (49 males and 32 females) died. Handgrip strength and leg extension strength at age 85 were stronger in surviving men than in non-survivors. Total mortality adjusted for both gender and serum level of total cholesterol fell 5-6% with a 1-kg increase in the handgrip strength of a single hand or both hands. Total mortality also decreased 2% with a 1 kg increase in the leg extension strength of both legs. With adjustment for gender and total cholesterol, mortality fell by 57% in participants of the walking test and fell by 45% in participants of the stepping-rate test compared to mortality in nonparticipants. No association was found between mortality and participation in the handgrip strength test, leg extension strength test, or one-leg standing time test. In conclusion, not only poor muscle strength in handgrip or leg extension, but also nonparticipation in walking test or leg-stepping test were independent predictors of total mortality in a very elderly population.


Subject(s)
Mortality , Physical Fitness , Aged, 80 and over , Female , Humans , Japan/epidemiology , Male , Residence Characteristics
4.
Cell Tissue Res ; 345(2): 243-52, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21739243

ABSTRACT

Taste receptors and their downstream signaling molecules are activated by sugars and sweeteners in the gut and participate in the regulation of glucose transport into enterocytes. The glucose transporter families GLUT and SGLT are responsible for the absorption of glucose, GLUT4 and SGLT1 being expressed preferentially in T1R3-positive taste cells. However, the expression patterns of the other glucose transporters in mouse gustatory tissues have not yet been elucidated. Therefore, we have examined the expression patterns of the glucose transporters (GLUT1-4 and SGLT1-3) in mouse gustatory tissues. Reverse transcription/polymerase chain reaction assays have revealed that GLUT1, 3, and 4 and SGLT1 mRNAs are expressed in the circumvallate papillae. Immunohistochemical analysis has shown that SGLT1 is expressed in a subset of the epithelial cells: from the basal cell layer to the prickle cell layer and in intragemmal and extragemmal epithelium cells in the circumvallate, foliate, and fungiform papillae. GLUT1, GLUT3, and GLUT4 are expressed in the prickle cell layers and/or basal cell layers in these papillae. Moreover, GLUT1, but not GLUT3 or GLUT4, is expressed in a subset of intragemmal and extragemmal epithelium cells in these papillae. Double-labeling experiments have demonstrated that GLUT1-positive taste bud cells coexpress gustducin and inositol 1,4,5-triphosphate receptor type III. These results suggest that SGLT1 and GLUT1 play a role in glucose-sensing and/or transport in mouse taste buds.


Subject(s)
Glucose Transport Proteins, Facilitative/biosynthesis , Taste Buds/metabolism , Tongue/metabolism , Animals , Glucose Transport Proteins, Facilitative/genetics , Immunohistochemistry , Inositol 1,4,5-Trisphosphate Receptors/metabolism , Male , Mice , Mice, Inbred C57BL , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Real-Time Polymerase Chain Reaction
5.
Dev Dyn ; 240(4): 775-84, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21322090

ABSTRACT

Mash1 is expressed in subsets of neuronal precursors in both the central nervous system and the peripheral nervous system. However, involvement of Mash1 in taste cell differentiation has not previously been demonstrated. In this study, we investigated the role of Mash1 in regulating taste bud differentiation using Mash1 KO mice to begin to understand the mechanisms that regulate taste bud cell differentiation. We found that aromatic L-amino acid decarboxylase (AADC) cells were not evident in either the circumvallate papilla epithelia or in taste buds in the soft palates of Mash1 KO mice. However gustducin was expressed in taste buds in the soft palates of Mash1 KO mice. These results suggest that Mash1 plays an important role in regulating the expression of AADC in type III cells in taste buds, which supports the hypothesis that different taste bud cell types have progenitor cells that are specific to each cell type.


Subject(s)
Aromatic-L-Amino-Acid Decarboxylases/metabolism , Basic Helix-Loop-Helix Transcription Factors/physiology , Cell Differentiation/genetics , Taste Buds/embryology , Taste Buds/metabolism , Animals , Basic Helix-Loop-Helix Transcription Factors/genetics , Basic Helix-Loop-Helix Transcription Factors/metabolism , Cells, Cultured , Embryo, Mammalian , Epithelium/embryology , Epithelium/metabolism , Female , Gene Expression Regulation, Developmental , Mice , Mice, Knockout , Palate/embryology , Palate/metabolism , Pregnancy
6.
J Biol Chem ; 286(11): 8952-60, 2011 Mar 18.
Article in English | MEDLINE | ID: mdl-21239499

ABSTRACT

The contribution of plasminogen (Plg)/plasmin, which have claimed to be the main fibrinolytic regulators in the bone metabolism, remains unclear. This study evaluated how the absence of Plg affects the function of osteoblast (OB) and osteoclast (OC). There was a larger population of pre-OCs in bone marrow-derived cells from the Plg(-/-) mice than the population of that from the WT mice. In addition, the absence of Plg suppressed the expression of osteoprotegerin in OBs. Moreover, an exogenous plasmin clearly induced the osteoprotegerin expression in Plg(-/-) OBs. The osteoclastogenesis of RAW264.7 mouse monocyte/macrophage lineage cells in co-culture with OBs from the Plg(-/-) mice was significantly accelerated in comparison with that in co-culture with OBs from the WT mice. Intriguingly, the accelerated OC differentiation of RAW264.7 cells co-cultured with Plg(-/-) OBs was clearly suppressed by the treatment of an exogenous plasmin. Consequently, Plg(-/-) mice display decreased bone mineral density. These findings could eventually lead to the development of new clinical therapies for bone disease caused by a disorder of the fibrinolytic system.


Subject(s)
Bone Density/physiology , Bone Marrow Cells/metabolism , Cell Differentiation/physiology , Fibrinolysin/metabolism , Osteoblasts/metabolism , Osteoclasts/metabolism , Plasminogen/metabolism , Animals , Bone Diseases/genetics , Bone Diseases/metabolism , Bone Marrow Cells/cytology , Bone and Bones/cytology , Bone and Bones/metabolism , Cell Line , Fibrinolysin/genetics , Mice , Mice, Knockout , Osteoblasts/cytology , Osteoclasts/cytology , Plasminogen/genetics
7.
Gerodontology ; 27(3): 199-206, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20491951

ABSTRACT

OBJECTIVE: The removal of adherent biofilms was assessed using ultrasonic waves in a non-contact mode. MATERIALS AND METHODS: In in vitro experiments, Streptococcus mutans (S. mutans) biofilms were exposed to ultrasonic waves at various frequencies (280 kHz, 1 MHz, or 2 MHz), duty ratios (0-90%), and exposure times (1-3 minutes), and the optimal conditions for biofilm removal were identified. Furthermore, the effect of adding a contrast medium, such as micro bubbles (Sonazoid), was examined. The spatial distribution and architecture of S. mutans biofilms before and after ultrasonic wave exposure were examined via scanning electron microscopy. The biofilm removal effect was also examined in in vivo experiments, using a custom-made oral cleaning device. RESULTS: When a 280 kHz probe was used, the biofilm-removing effect increased significantly compared to 1 and 2 MHz probes; more than 80% of the adherent biofilm was removed with a duty cycle of 50-90% and a 3 minutes exposure time. The maximum biofilm-removing effect was observed with a duty cycle of 80%. Furthermore, the addition of micro bubbles enhanced this biofilm-removing effect. In in vivo experiments, moderate biofilm removal was observed when a 280 kHz probe was used for 5 minutes. CONCLUSIONS: This study demonstrated that ultrasonic wave exposure in a non-contact mode effectively removed adherent biofilms composed of S. mutans in vitro.


Subject(s)
Biofilms , Streptococcus mutans/physiology , Ultrasonics , Bacteriological Techniques , Coloring Agents , Dental Plaque/microbiology , Dental Plaque/therapy , Ferric Compounds/therapeutic use , Humans , Iron/therapeutic use , Materials Testing , Microbubbles/therapeutic use , Microscopy, Electron, Scanning , Oxides/therapeutic use , Spectrophotometry , Streptococcus mutans/ultrastructure , Time Factors , Transducers , Ultrasonics/classification
8.
Arch Gerontol Geriatr ; 50(3): 272-6, 2010.
Article in English | MEDLINE | ID: mdl-19419777

ABSTRACT

Since little is known about the very elderly population aged 80 years and older, we evaluated the association of quality of life (QoL) in an 85-year-old population with physical fitness measurements assessed at age 80 and 85 years. Two hundred seven individuals (90 males, 117 females) aged 85 years underwent the Short Form-36 (SF-36) questionnaires for QoL assessment and physical fitness measurements (handgrip strength, leg-extensor strength, one-leg standing time, stepping rate of legs, walking speed). In 85-year-olds, significant associations were found, by multiple regression analysis or logistic regression analysis, with adjustment for various influencing factors in QoL assessed by SF-36 with physical fitness measurements examined at the age of 85 and 80 years. Physical scales and scores in SF-36, such as physical functioning (PF), limitation in role functioning for physical reasons (role physical; RP), bodily pain (BP), and the physical component score (PCS) tended to be more tightly associated with fitness measurements than mental scales and scores such as limitation in role functioning for emotional reasons (role emotional; RE), and emotional well-being (mental health; MH), and mental component score (MCS). Three scales the general health perceptions (GH), the vitality (VT), and the social functioning (SF) consisting of both physical and mental components were associated with fitness, the extent being intermediate between physical scales and mental scales. Of the several physical fitness measurements, leg-extensor strength and the walking speed of 85-year-olds, and the stepping rate of 80-year-olds were most closely associated with QoL. In a very elderly population of 85- and 80-year-olds, significant associations were found between QoL by SF-36 and physical fitness measurements, suggesting that increases in the levels of physical fitness, even in the very elderly, can contribute to improvements in QoL.


Subject(s)
Health Status , Physical Fitness , Quality of Life , Aged, 80 and over , Female , Health Surveys , Humans , Japan , Logistic Models , Male , Multivariate Analysis
9.
J Atheroscler Thromb ; 16(5): 691-7, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19729867

ABSTRACT

AIM: Helicobacter pylori (HP) has been implicated as a risk factor for cardiovascular and atherosclerotic diseases. Arterial stiffness determined by pulse wave velocity (PWV) or the cardio-ankle vascular index (CAVI) has been shown to be higher in HP-positive subjects than in HP-negative subjects; however, this result has been observed only in young subjects. The aim of the study was to investigate the possible correlation between HP infection and PWV or CAVI in middle-aged subjects. METHODS: We measured brachial-ankle PWV (baPWV), CAVI, metabolism markers, pepsinogens (PGs) and IgG antibody to HP in 343 individuals aged either 60 or 65 year old. Atrophic gastritis (AG) was diagnosed based on the values of PGs. RESULTS: baPWV and CAVI were significantly higher in the AG-positive group than in the AG-negative group even after adjusting for possible confounding factors (baPWVc; 16.63+/-3.50 vs. 15.59+/-3.47 p=0.010, CAVIc; 8.59+/-1.20 vs. 8.27+/-1.19 p=0.022). baPWV and CAVI values tended to be higher in the HP-positive group than in the HP-negative group. High-density lipoprotein (HDL) cholesterol level and the adiponectin level were lower in the AG-positive group than in the AG-negative group. CONCLUSION: There may be an association between atrophic gastritis and atherosclerosis in middle-aged subjects.


Subject(s)
Arteries/physiopathology , Gastritis, Atrophic/complications , Vascular Diseases/complications , Aged , Compliance , Enzyme-Linked Immunosorbent Assay , Female , Gastritis, Atrophic/microbiology , Helicobacter pylori/isolation & purification , Humans , Japan , Male , Middle Aged , Vascular Diseases/physiopathology
10.
J Oral Pathol Med ; 38(7): 572-9, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19549112

ABSTRACT

Ultrasound-mediated destruction of microbubbles has been proposed as an innovative non-invasive drug delivery system for cancer therapy. We developed a specific drug delivery system for squamous cell carcinoma that uses sonoporation with the anti-epidermal growth factor receptor (EGFR) antibody. Administration of a low dose of bleomycin (BLM) by sonoporation with the anti-EGFR antibody produced a marked growth inhibition of Ca9-22 cells in vitro. In addition, scanning electron microscopic analysis revealed apparent surface deformation of Ca9-22 cells treated with sonoporation in the presence of the antibody. Interestingly, the population of apoptotic cells was remarkably increased when a low dose of BLM was delivered using sonoporation with the Fab fragment of the anti-EGFR antibody. These findings indicate that sonoporation with the Fab fragment makes it possible to administer drugs into cells more efficiently and specifically, suggesting a novel application for chemotherapy and gene therapy treatments for oral squamous cell carcinoma.


Subject(s)
Antineoplastic Agents/administration & dosage , Bleomycin/administration & dosage , Carcinoma, Squamous Cell/therapy , Drug Delivery Systems/methods , Gingival Neoplasms/therapy , Immunotoxins/administration & dosage , Antibodies/administration & dosage , Carcinoma, Squamous Cell/pathology , Combined Modality Therapy , ErbB Receptors/immunology , Gingival Neoplasms/pathology , Humans , Immunoglobulin Fab Fragments/administration & dosage , Immunoglobulin Fab Fragments/immunology , Microbubbles , Tumor Cells, Cultured , Ultrasonics
11.
Biochim Biophys Acta ; 1769(11-12): 641-8, 2007.
Article in English | MEDLINE | ID: mdl-17928076

ABSTRACT

The T1R family (T1R1, T1R2 and T1R3 receptors) has a role in the detection of umami and sweet tastes in taste buds. Although T1R3 is also expressed in the intrahepatic bile duct, the expression patterns of T1R1 and T1R2 in this region have not been determined. In addition, the mechanisms of transcriptional regulation of the human T1R3 gene (Tas1r3) have not been elucidated. In this study, we determined the expression patterns of T1R2 and T1R3 in human liver and the function of C/EBPbeta in Tas1r3 promoter activity. Immunohistochemistry showed that T1R2 and T1R3 were expressed in the intrahepatic bile duct. 5'-RACE analysis revealed that the transcriptional start sites of Tas1r3 were located 67 bp and 176 bp upstream of the ATG. Promoter analysis of Tas1r3 was performed using the luciferase reporter assay and EMSA in the Tas1r3-expressing cell line, HuCCT1. The 226-bp region upstream of the ATG had promoter activity, and C/EBPbeta activated the Tas1r3 promoter activity in HuCCT1 cells. These results show that T1R2 and T1R3 receptors, in addition to their role in taste perception, may also have a role in intrahepatic cholangiocytes. C/EBPbeta was identified as the transcription factor regulating Tas1r3 promoter activity in HuCCT1 cells.


Subject(s)
Bile Duct Neoplasms/genetics , CCAAT-Enhancer-Binding Protein-beta/physiology , Carcinoma/genetics , Gene Expression Regulation , Receptors, G-Protein-Coupled/genetics , 5' Flanking Region , Bile Duct Neoplasms/metabolism , Binding Sites , Cell Line, Tumor , Humans , Immunohistochemistry , Liver/metabolism , Promoter Regions, Genetic , Transcription Initiation Site , Transcriptional Activation
12.
Histochem Cell Biol ; 127(4): 415-22, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17211625

ABSTRACT

Aromatic L-amino acid decarboxylase (AADC) catalyses the decarboxylation of all aromatic L-amino acids. In mammals, AADC is expressed in many tissues besides the nervous system, and is associated with additional regulatory roles of dopamine and serotonin in a wide range of tissues. We examined the expression of AADC by using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. RT-PCR analysis showed that mRNA of AADC was detected in the taste bud-containing epithelium of the circumvallate papilla of mice. By immunohistochemical analyses, AADC was detected in a subset of taste bud cells of fungiform, foliate, and circumvallate papillae. Double-label studies showed that AADC colocalized with serotonin, NCAM, PLCbeta2, and PGP9.5. On the other hand, AADC never colocalized with alpha-gustducin. Our results of double staining with AADC and taste cell markers indicate that only the type III cells could convert 5-hydroxytryptophan (5-HTP) to serotonin within taste buds. Taken together with previous studies, the properties of the type III cell of taste buds exactly fit into the APUD (amine and amine precursor uptake and decarboxylation) cell scheme. Furthermore, in the developing circumvallate papilla, AADC are first detected in a small number of papillary epithelial cells at E14.5. By E18.5, AADC-positive epithelial cells also express PGP9.5, which is one of marker of taste cells, and these cells have been contacted by developing nerve fibers. These results suggest that AADC expression begins at early stages of taste bud cell differentiation, and biogenic amines may act on taste bud differentiation of tongue epithelial cells, and further may regulate innervation of taste bud progenitor cells.


Subject(s)
Aromatic-L-Amino-Acid Decarboxylases/metabolism , Taste Buds/metabolism , Animals , Aromatic-L-Amino-Acid Decarboxylases/genetics , Biomarkers/analysis , Female , Gene Expression Regulation, Developmental , Gene Expression Regulation, Enzymologic , Immunohistochemistry , Male , Mice , Mice, Inbred ICR , Pregnancy , Reverse Transcriptase Polymerase Chain Reaction , Serotonin/metabolism , Taste Buds/embryology , Taste Buds/growth & development , Ubiquitin Thiolesterase/analysis
13.
Cell Tissue Res ; 328(1): 57-63, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17216195

ABSTRACT

Glutamate is one candidate for the neurotransmitters and/or neuromodulators involved in taste signaling in taste buds. Group II metabotropic glutamate receptors (mGluRs: mGluR2 and mGluR3) are known to function as presynaptic receptors that regulate the release of glutamate and/or other neurotransmitters in the central nervous system. Group II mGluRs are negatively linked to adenylyl cyclase through Galphai subunits and thereby reduce the turnover of cAMP. In rat taste tissues, a subset of adenylyl-cyclase-8-expressing taste cells coexpress the Galphai subunits gustducin and Galphai2. However, the expression patterns of group II mGluRs in rat taste tissues have not yet been elucidated. We have therefore examined the expression patterns of mGluR2, mGluR3, and gustducin in rat gustatory tissues. Reverse transcription/polymerase chain reaction assays have revealed that mGluR2 and mGluR3 mRNAs are expressed in the circumvallate papillae. In situ hybridization analyses have detected positive signals for mGluR2 and mGluR3 mRNAs only in the circumvallate taste buds. Among the fungiform, foliate, and circumvallate papillae, an antibody against mGluR2/3 labels a subset of taste bud cells and nerve fibers immediately beneath the taste lingual epithelium. Double-labeling experiments have demonstrated that mGluR2/3-positive cells coexpress gustducin. These results indicate that mGluR2 and mGluR3 are coupled to Galphai subunits and play roles in glutamate-mediated signaling in taste transductions.


Subject(s)
Gene Expression Regulation , Receptors, Metabotropic Glutamate/genetics , Receptors, Metabotropic Glutamate/metabolism , Taste Buds/metabolism , Animals , Immunohistochemistry , In Situ Hybridization , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Reverse Transcriptase Polymerase Chain Reaction , Taste Buds/cytology , Transducin/metabolism
14.
Arch Histol Cytol ; 70(4): 215-24, 2007 Nov.
Article in English | MEDLINE | ID: mdl-18296822

ABSTRACT

The present study demonstrated for the first time the localizations and patterns of expression of key enzymes for steroidogenesis, cytochrome P450 side-chain-cleavage (P450scc), and P450 aromatase in the taste buds of rat circumvallate papillae, using immunoblot analyses and immunohistochemistry. Immunoblot analyses showed that proteins with a molecular weight close to that of rat adrenal cytochrome P450scc and a molecular weight close to that of rat ovary cytochrome P450 aromatase were present in the rat circumvallate papillae. In immunohistochemistry, antibodies against cytochrome P450scc and P450 aromatase yielded the labelings of a subset of taste bud cells. In the double immunolabeling of P450scc and alpha-gustducin or phospholipase C beta2(PLCbeta2), which were considered as markers of a majority of type II cells, P450scc was co-expressed in a subset of alpha-gustducin or PLCbeta2, but did not co-express neural adhesion molecule (NCAM), a marker of major type III cells. Further double immunolabeled studies showed that P450 aromatase was co-expressed in a subset of alpha-gustducin or PLCbeta2, but did not co-express PGP9.5, a marker of a majority of type III cells. The selective localization of cytochrome P450scc and P450 aromatase strongly suggests that estrogen biosynthesis from cholesterol might occur in a subset of type II cells of the rat taste buds. Although the full significance of estrogen in the taste bud function is not yet understand, estrogen appears to be an important regulator of taste transduction, as is the case with ATP (Finger et al., 2005), which further supports the centrality of taste cells in the life of taste buds.


Subject(s)
Aromatase/metabolism , Cytochrome P-450 Enzyme System/metabolism , Taste Buds/enzymology , Animals , Female , Gonadal Steroid Hormones/metabolism , Immunoblotting , Immunohistochemistry , Male , Neural Cell Adhesion Molecules/metabolism , Phospholipase C beta/metabolism , Rats , Rats, Sprague-Dawley , Taste Buds/cytology , Transducin/metabolism , Ubiquitin Thiolesterase/metabolism
15.
Arch Histol Cytol ; 69(3): 189-98, 2006 Sep.
Article in English | MEDLINE | ID: mdl-17031025

ABSTRACT

Taste buds are multicellular receptor organs embedded in the lingual epithelium of vertebrates. Taste cells within these buds are modified epithelial cells as they lack axons and turnover rapidly throughout life, yet have neuronal properties enabling them to transduce taste stimuli and transmit this information to the nervous system. Taste cells are heterogeneous, comprising types I, II, III and basal cells, and are continually replaced during adult life, raising the question of how these different cells are generated. The molecular mechanisms governing taste cell differentiation are unknown, but the Notch signaling system has been implicated in this process based upon recent gene expression data. Here we investigate the expression in mature taste buds of Notch related transcription factors, Hes6 and Mash1, which are among the first genes expressed in embryonic taste buds. We further compare these patterns with those of immunocytochemical markers of discrete taste cell types. We find that Hes6 is expressed in a subset of basally located, possibly progenitor cells, yet is rarely coexpressed with taste cell markers. In contrast, Mash1 is detected in some basal cells and in the majority of differentiated type III taste cells, but never in type II cells. These data suggest a role for Notch signaling in taste cell differentiation in adult taste buds.


Subject(s)
Basic Helix-Loop-Helix Transcription Factors/biosynthesis , Repressor Proteins/biosynthesis , Taste Buds/cytology , Taste Buds/metabolism , Animals , Cell Differentiation/physiology , Fluorescent Antibody Technique , Immunohistochemistry , In Situ Hybridization , Mice
16.
Arch Histol Cytol ; 69(4): 273-80, 2006 Dec.
Article in English | MEDLINE | ID: mdl-17287581

ABSTRACT

Galanin, a 29-amino-acid neuropeptide, was initially isolated from porcine intestine. It has a wide spread distribution in the central nervous system and is also present in the primary sensory neuron. Galanin has been suggested to be involved in numerous neuronal and endocrine functions as a neurotransmitter and neuromodulator. We examined the expression of galanin and galanin receptors by using a reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, and in situ hybridization. RT-PCR analysis showed that mRNA of galanin and GalR2 were detected in the taste bud-containing epithelium of the circumvallate papilla of rats. Immunohistochemical analyses detected galanin was detected in a subset of taste bud cells of the circumvallate papillae. Double-label studies showed that galanin colocalized with alpha-gustducin, NCAM, and PLCbeta2. Our results of double staining with galanin and taste cell markers indicate that galanin-expressing taste cells are type II and type III cells. Taken together with previous studies, these findings show that galanin may function as a taste bud neurotransmitter. Furthermore, GalR2 mRNA was expressed in some taste bud cells. This suggests that, galanin release may not only excite the peripheral afferent nerve fiber but also may act on neighboring taste receptor cells via the activation of GalR2.


Subject(s)
Galanin/biosynthesis , Receptors, Galanin/biosynthesis , Taste Buds/metabolism , Actins/biosynthesis , Animals , Female , Image Processing, Computer-Assisted , Immunohistochemistry , In Situ Hybridization , Isoenzymes/biosynthesis , Male , Neural Cell Adhesion Molecules/biosynthesis , Phospholipase C beta , RNA, Messenger/biosynthesis , Rats , Rats, Sprague-Dawley , Reverse Transcriptase Polymerase Chain Reaction , Taste/physiology , Taste Buds/cytology , Transducin/biosynthesis , Type C Phospholipases/biosynthesis
17.
Arch Histol Cytol ; 69(4): 281-8, 2006 Dec.
Article in English | MEDLINE | ID: mdl-17287582

ABSTRACT

It has recently become evident that ATP and other extracellular nucleotides could play an important role in signal transductions. ATP mediates excitatory signaling by means of P2X receptors. P2X3, one of its subtypes, a membrane ligand-gated ion channel, is strongly expressed in peripheral sensory neurons. The aim of the present study was to examine the distribution of nerve fibers expressing P2X3 receptors in taste buds in the gustatory papillae and soft palate of rats by immunohistochemistry. We found that the fluorescence ATP marker quinacrine stained subsets of taste bud cells. Numerous nerve fibers innervating taste buds were intensely immunostained with the P2X3 receptor antibody. These nerve fibers ascended among intragemmal cells and terminated just below the taste pores. In order to examine whether P2X3 receptors are involved in signal modulation within taste buds, we used fluorescent double stainings to analyze the distribution of P2X3 receptors and their relationship to alpha-gustducin immunopositive taste receptor cells. Many varicose nerve fibers expressing P2X3 receptor-immunoreactivities were entangled with alpha-gustducin-immunopositive taste receptor cells and ended closely below the taste pores. In fungiform papillae, nerve fibers expressing both P2X3 receptors and PGP 9.5 were observed. In contrast, only PGP 9.5 immunoreactive nerve fibers were recognized in filiform papillae. These results suggest that P2X3 receptors might be involved in taste transmission pathways within taste buds. ATP may act as a neurotransmitter, co-transmitter, or neuromodulator at P2X3 receptors to generate activating gustatory nerve fibers.


Subject(s)
Adenosine Triphosphate/physiology , Receptors, Purinergic P2/biosynthesis , Taste Buds/metabolism , Animals , Female , Fluorescent Antibody Technique , Male , Quinacrine/pharmacology , Rats , Rats, Sprague-Dawley , Receptors, Purinergic P2X3 , Signal Transduction/genetics , Signal Transduction/physiology , Taste/physiology , Taste Buds/cytology , Transducin/metabolism , Ubiquitin Thiolesterase/genetics , Ubiquitin Thiolesterase/physiology
18.
Arch Oral Biol ; 50(8): 695-705, 2005 Aug.
Article in English | MEDLINE | ID: mdl-15958201

ABSTRACT

In developing teeth, dental epithelial progenitor cells differentiate through sequential and reciprocal interactions with neural-crest-derived mesenchyme. However, the molecular mechanisms involved in cell differentiation are not well understood. Continuously growing teeth are useful in the study of differentiation of dental progenitor cells. In rat lower incisors, ameloblasts originate from the dental epithelial adult stem cell compartment referred to as the 'apical bud'. To elucidate the mechanism of ameloblast differentiation, we designed a primary culture system and confirmed the differentiation of dental epithelial cells through interaction with mesenchymal cells. Cytokeratin was used as a marker for epithelial cells, nerve growth factor receptor p75 for inner enamel epithelial (IEE) cells, and ameloblastin for ameloblasts. The apical bud cells could only differentiate into IEE cells and, within 10 days, into ameloblasts expressing ameloblastin in the presence of dental papilla cells. Interestingly, the IEE cells could proliferate transiently and differentiate into ameloblasts in the presence or absence of dental papilla cells. These results suggest that apical bud cells can enter the ameloblast cell lineage through interaction with mesenchymal cells. IEE cells, on the other hand, are already committed to differentiate into ameloblasts. This culture system is useful in future studies of ameloblast differentiation.


Subject(s)
Cell Differentiation/physiology , Incisor/cytology , Stem Cells/physiology , Ameloblasts/physiology , Animals , Cell Count/methods , Cell Division/physiology , Cells, Cultured , Dental Enamel Proteins/analysis , Dental Papilla/cytology , Epithelial Cells/physiology , Immunohistochemistry/methods , Keratins/analysis , Mesenchymal Stem Cells/physiology , Rats , Rats, Wistar , Receptor, Nerve Growth Factor/analysis
19.
Infect Immun ; 73(6): 3512-20, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15908380

ABSTRACT

We identified a gene (atlA) encoding autolytic activity from Streptococcus mutans Xc. The AtlA protein predicted to be encoded by atlA is composed of 979 amino acids with a molecular weight of 107,279 and has a conserved beta-1,4-N-acetylmuramidase (lysozyme) domain in the C-terminal portion. Sodium dodecyl sulfate extracts of strain Xc showed two major bacteriolytic bands with molecular masses of 107 and 79 kDa, both of which were absent from a mutant with inactivated atlA. Western blot analysis revealed that the 79-kDa band was derived from the 107-kDa peptide by cleavage of its N-terminal portion. The inactivation of atlA resulted in a marked decrease in autolysis and the formation of very long chains of cells compared to the case for the parent strain. Although both the parent and mutant strains formed biofilms in the presence of sucrose, the biofilms formed by the mutant had a sponge-like architecture with large gaps and contained 30% less biomass than those formed by the parent strain. Furthermore, strain Xc formed glucose-dependent, loose biofilms in the absence of sucrose, but the mutant lost this ability. These results suggest that AtlA may play an important role in biofilm formation by S. mutans. The antibody produced against the C-terminal peptide containing the beta-1,4-N-acetylmuramidase domain drastically inhibited the autolytic activity of strain Xc. This inhibition was specific among the oral streptococci to S. mutans. These results indicate that the catalytic domain of AtlA is located at the C terminus, suggesting that further characterization of this domain may provide a means to control cariogenic dental plaque formation.


Subject(s)
N-Acetylmuramoyl-L-alanine Amidase/genetics , Streptococcus mutans/genetics , Base Sequence , Biofilms/growth & development , Catalytic Domain , Cloning, Molecular , Molecular Sequence Data , N-Acetylmuramoyl-L-alanine Amidase/chemistry , N-Acetylmuramoyl-L-alanine Amidase/physiology
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