ABSTRACT
The clearance and production rates of plasma angiotensinogen of normal dog, nephrectomized dog and angiotensin converting enzyme inhibitor (ACEI) administered dog were calculated. Plasma angiotensinogen was measured by adding excessive dog renin to the plasma sample. The clearance rate was 4.4, 4.0 and 3.8 ml/kg/hr and the production rate was 9.6, 21.6 and 7.6 micrograms/kg/hr, respectively. After nephrectomy, the clearance rate of angiotensinogen decreased only 10 percent but the production rate increased more than twice. These results mean that the kidney does not play an important role in the clearance of angiotensinogen but has some influence on its production. This increase in production rate due to nephrectomy may mean that the kidney has some inhibitory effect on the secretion of angiotensinogen from the liver but this phenomenon is not able to explain by much less hydrolysis of angiotensinogen. By the administration of ACEI, not only the clearance rate but also the production rate of plasma angiotensinogen decreased as compared with control dog, but the balance between them resulted in the decreased plasma angiotensinogen concentration. These results show that the decreased production rate was greater than the decreased clearance rate in ACEI administered dog.
Subject(s)
Angiotensinogen/biosynthesis , Enalapril/pharmacology , Nephrectomy , Angiotensinogen/blood , Angiotensinogen/metabolism , Animals , Chromatography, Affinity , Chromatography, Gel , Dogs , Electrophoresis, Polyacrylamide Gel , Kidney/physiology , Liver/metabolismSubject(s)
Angiotensinogen/blood , Enalapril/pharmacology , Hypertension/blood , Angiotensin I/blood , Animals , Blood Pressure/drug effects , Male , Rats , Rats, Inbred SHRABSTRACT
The urinary aldosterone (ALD) was measured by aldosterone RIA kit and the following results were obtained. (1) We have known that ten percent solution of bovine serum albumin (BSA) instead of free serum LAD is used as the diluent of the urine in aldosterone RIA kit. (2) The upper limits of free ALD, HCl-ALD and beta-glucuronidase ALD in the urine diluted with 10% BSA were 1.6, 9.0 and 9.5 micrograms/dl respectively. (3) The values of urinary conjugated HCl-ALD and beta-glucuronidase ALD were approximately 3.5 times and 4 times as much as that of free ALD respectively. (4) A good correlation was obtained among the results of three methods (HCl-ALD, beta-glucuronidase ALD and free ALD). (5) No difference was found in the values of the urinary ALD in the healthy subjects and the patients with essential hypertension, kidney diseases and acute liver diseases.
