Prevalence of Coxiella burnetii antibodies in wild ruminants in Kavecany zoo, Kosice, eastern Slovakia.

Coxiella burnetii is an obligate intracellular agent that causes Q fever in humans and animals. The most important reservoirs of C. burnetii in nature are small wild rodents, but infection was also demonstrated in other animals, including ruminants. Samples of blood were obtained from 4 mouflons, 60 fallow deer, 9 Cameroun goats, 8 Carpathian goats, and 8 Cameroun sheep living in a zoo. Antibodies to phase I and phase II C. burnetii antigens were determined in sera by ELISA. Antibody titres were detected in the range 1:100-1:200. The serum prevalence of phase II and phase I antibodies to C. burnetii antigens was 25 % and 0% in mouflons, 70 % and 0% in goats, 37.5 % and 12.5 % in sheep and 28.3 % and 5 % in fallow deer, resp. Serologic diagnosis of Q fever in animals can be difficult. Some animals may shed C. burnetii and pose a risk for infection prior to the development of antibodies, and some infected animals never seroconvert. The employed ELISA test is a very sensitive assay for C. burnetii, but it is also a labour intensive method and therefore not routinely available.

Sensitivity of Borrelia genospecies to serum complement from different animals and human: a host-pathogen relationship.

Different Borrelia species and serotypes were tested for their sensitivity to serum complement from various animals and human. Complement-mediated Borrelia killing in cattle, European bison and deer was higher irrespective of the Borrelia species whereas in other animals and human it was intermediate and Borrelia species-dependent. Activation of the alternative complement pathway by particular Borrelia strain was in correlation with its sensitivity or resistance. These results support the incompetent reservoir nature of cattle, European bison, red, roe and fallow deer, at the same time present the probable reservoir nature of mouflon, dog, wolf, cat and lynx. In short, this study reviews Borrelia-host relationship and its relevance in reservoir competence nature of animals.

Small mammals (Insectivora, Rodentia) as a potential source of chlamydial infection in East Slovakia.

The presence of antibodies against Chlamydophila (Chlamydia) psittaci in small mammals (Insectivora, Rodentia) in the region of East Slovakia are presented. The hosts were caught in several areas of Slovakia in habitats with different levels of anthropogenic disturbance. Research was carried out during 2000-2002. The authors examined 1,947 sera coming from 4 insectivore and 10 rodent species. Each serum was examined by micromethod of complement binding reactions using antigen Chlamydophila (Chlamydia) psittaci. Chlamydial infections were found in 251 individuals (prevalence 12.9 %) of 8 mammal species. The antichlamydial antibodies were proved at levels ranging from 1:32-1:1024. The highest prevalence of antibodies was detected in the most abundant rodent species Apodemus microps (14.8 %), Apodemus agrarius (13.9 %), Apodemus flavicolis (12.4 %), Microtus arvalis (12 %), and Clethrionomys glareolus (10.9 %). Positive hosts were registered in all studied localities. Testing of prevalence values in the individual research years confirmed significant changes. Our results showed that small mammals probably play an important role in the circulation of chlamydiae in nature.

Anti-Borrelia antibodies in rodents: important hosts in ecology of Lyme disease.

Data presented in this study focuses on the presence of anti-Borrelia antibodies in small mammals from Eastern Slovakia during 2000-2003. The total seropositivity observed was 18.78% in rodents. Amongst all species, the total seroprevalence in Apodemus flavicolis was the highest (20.87%), followed by Apodemus agrarius (19.58%) and Clethrionomys glareolus (11.11%). However, the prevalence in Apodemus flavicolis during the year 2000-2001 was higher (26.72%), which reduced to 10.60% in 2002-2003. To compare the year range of seroprevalence in other small mammals was not feasible due to the small sample number. Area-wise distribution of anti-Borrelia antibodies was even (18.75% to 20%) in this study, except in the Bot'any province (0%). This confirms the equal distribution of Borrelia spirochetes in the other 3 localities. Prevalence of anti-Borrelia antibodies during summer was significantly higher than during autumn and early spring. The overall study also reviews the importance of small mammals in Lyme disease ecology.

Serological screening of occurrence of antibodies to Encephalitozoon cuniculi in humans and animals in Eastern Slovakia.

Encephalitozoon cuniculi is one of the mamalian microsporidian pathogens that can affect a number of different species of animals as well as humans. The presence of specific serum antibodies to Encephalitozoon cuniculi was studied in a group of animals and humans from Eastern Slovakia by the indirect immunofluorescence of antibodies (IFA). 456 people, 571 rabbits, 457 mice, 193 dogs, 72 cats, and 59 sheep were examined. Specific anti-E. cuniculi antibodies were found in 26 out of 456 human sera examined (5.7%). The highest occurrence of antimicrosporidial antibodies was found in the group of immunodeficiency patients - 37.5%. In the group of animals, the highest positivity was observed in rabbits - 41.7%, and in dogs - 37.8. The relative high prevalence, especially in rabbits and dogs as potential sources of microsporidial infection for humans, indicates the importance of performing the screening examinations in animals with aim of reducing or halting the spread of this disease.

Seroprevalence of anti-Borrelia burgdorferi antibodies in sheep and goats from mountainous areas of Slovakia.

In the present study, domestic animals such as sheep and goats from eastern Slovakia were screened for the presence of anti-Borrelia antibodies. Seroprevalence in 181 sheep and 65 goats were carried out in 1999 and 2000. Modified ELISA method was used for detection of anti-Borrelia IgG antibodies. Seroprevalence obtained was 15.8% and 17.5% in 1999 and 2000 respectively in sheep, whereas in goats it was 17.2% and 19.4% respectively. The results suggest that these domestic species have potential to transmit the disease to other animals. Though the role of sheep and goats in Lyme disease has not yet been documented, there is great possibility of transmission of the causative agent via co-feeding to human beings.

Wild pigeons and pheasants--a source of Chlamydophila psittaci for humans and animals.

The authors present results of serological examination in 275 pheasants (Phaisanus colchicus) and 273 pigeons (Columba livia f. domestica) for the presence of Chlamydophila (Ch) psittaci IgG antibodies. Using micromethod of complement fixation (CF) test with genus-specific antigen Ch. psittaci (Bioveta, Ivanovice na Hané, Czech Republic), the seropositivity in pheasants oscillated between 31.5-40.4 %. No clinical signs of chlamydiosis were detected in pheasants under study. The seropositivity in pigeons ranged between 33.1-85.1%. Total 77.1% positivity with maximal 1:1024 antibody titre was found in 83 pigeons caught in April 2000, while, in June 2000 positivity was 41.0% with maximum titre 1: 512. Similarly, in the year 2001 the seropositivity in the group of 74 pigeons trapped in April reached up to 85.1% with the highest titre 1:1024 and in the pigeons trapped in June positivity decreased to 33.3% with the titer 1:256. These results prove an acute form of chlamydiosis and suggest that pigeons in spring time are an especially significant source of chlamydiosis for the human and animal population.