[Effect of intravenous administration of dalargin on the course and outcome of the body's response to acute massive hemorrhage]. / Vliianie vnutrivennogo vvedeniia dalargina na techenie i iskhod reaktsii organizma na ostruiu massivnuiu krovopoteriu.

Acute experiments on 74 adult rabbits have revealed that slow fractional jet blood exfusion of 16 +/- 1 ml/kg for 4-5 min results in reversible hemorrhagic shock, but when the volume of blood loss increases up to 23 +/- 3 ml/kg, a fatal shock occurs. The same outcome occurs when one-stage continuous jet blood loss reaches the volume of 16 +/- 3 ml/kg. Preadministration of dalargin, 0.1 mg/kg prior to fatal hemorrhage promotes the survival of most animals and significantly prolongs their longevity. The therapeutical effect of dalargin in increasing the animals' life is shown only when it is used in early shock after fractional hemorrhage. In late shock, the agent fails to affect the further course and outcomes of posthemorrhagic responses after fractional or continuous blood loss.

[The reinfusion of blood and the administration of dalargin in experimental hemorrhagic shock]. / Reinfuziia krovi i vnutrivennoe vvedenie dalargina pri éksperimental'nom gemorragicheskom shoke.

In acute experiment on 95 adult rabbits it was determined that during the subdivided blood exfusion from femoral artery in the volume of (16 + 1) ml/kg the reversible hemorrhagic shock occurs and the animals survive without the hemorrhage compensation. The increase of hemorrhage volume up to (23 + 3) ml/kg leads to lethal hemorrhagic shock. The heparinized blood reinfusion in the early period promotes the survival of the whole of animals, in the late period it promotes trustworthy their life span increase. Under the influence of dalargin intravenous infusion in the dose of 0.1 mg/kg after the blood reinfusion in the late period of shock all the animals survived. Intravenous infusion of dalargin is expedient for the increase of the transfusion therapy effectiveness in the late period of hemorrhagic shock.

[Intravenous dalargin as a method of increasing the effectiveness of blood reinfusion in the late stages of hemorrhagic shock]. / Vnutrivennye vvedeniia dalargina kak metod povysheniia éffektivnosti reinfuzii krovi v pozdnem periode gemorragicheskogo shoka.

Acute experiments on 63 adult rabbits have shown that acute divided blood loss for 5 min (27 +/- 1% of the whole blood volume) results in the development of reversible hemorrhagic shock with survival of all the animals. In the same volume of continuous blood exfusion (27 +/- 5% of blood volume) or an increase in blood loss to 43 +/- 2% of blood volume hemorrhagic shock proved irreversible. Early reinfusion of heparinized blood saved all the animals, whereas late reinfusions only prolonged the survival. Intravenous administration of dalargin (0.1 mg/kg) in late shock was not effective and all the animals died. Dalargin introduction after blood reinfusion in late shock saved the animals lives. The conclusion is made on benefits of intravenous dalargin for raising the efficacy of reinfusions (hemotransfusion) at late stages of severe hemorrhagic shock.

[Changes in the enzyme activity of flavinogenesis in the process of culturing the fungus Eremothecium ashbyii]. / Izmenenie aktivnosti nekotorykh fermentov flavinogeneza v protsesse kul'tivirovaniia griba Eremothecium ashbyii.

The activity of enzymes involved in the beginning (GTP cyclohydrolase) and terminal steps (riboflavin synthase EC 2.5.1.9, riboflavin kinase EC 2.7.1.26 and FMN adenyltransferase EC 2.7.7.2) of flavinogenesis was studied in the mycelium of Erenmothecium ashbyii of different age. The activity of GTP cyclohydrolas, riboflavin kinase and FMN adenyltransferase was low in the young mycelium and increased in the process of growth, which was accompanied by the acceleration of flavinogenesis. The activity of riboflavin synthase was high in the young mycelium and changed only slightly in the process of subsequent cultivation of the fungus. 8-Azaadenine and 8-hydroxyquinoline added to the young culture of E. ashbyii inhibited the flavinogenesis of the mycelium and the increase of the enzyme activity.

[Activities of some yeast flavogenic enzymes in situ]. / Izuchenie aktivnosti nekotorykh fermentov flavinogeneza u drozhzhei in situ.

Effects of digitonin, dimethylsulfoxide and protamine sulfate on yeast Pichia guilliermondii were studied in order to produce cells with increased permeability and possessing the GTP-cyclohydrolase, riboflavinsynthetase and riboflavinkinase activities. The digitonin-treated cells exhibited a higher cyclohydrolase activity than the cell-free extracts; the activities of riboflavinsynthetase and riboflavinkinase in the cells and cell-free extracts were found to be similar. Treatment of cells with dimethylsulfoxide proved to be most effective to determine the activity of GTP-cyclohydrolase and also helpful to determine that of riboflavinsynthetase. Protamine sulfate had no effect on the cells of P. guilliermondii. The methods developed were used to determine the activities of GTP-cyclohydrolase, riboflavinsynthetase and riboflavinkinase in the cells of flavinogenic (P. guiller-mondii, Torulopsis candida) and non-flavinogenic (Candida utilis, Candida pulcherrima) yeasts grown in iron-rich and iron-deficient media. Derepression of riboflavinsynthetase and GTP-cyclohydrolase syntheses under conditions of Fe deficiency in the flavinogenic yeast cells confirmed previously made assumptions.