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1.
Arch Virol ; 165(12): 2921-2926, 2020 Dec.
Article in English | MEDLINE | ID: mdl-32989573

ABSTRACT

In this study, we present an analysis of metagenome sequences obtained from a filtrate of a siphon tissue homogenate of otter clams (Lutraria rhynchaena) with swollen-siphon disease. The viral signal was mined from the metagenomic data, and a novel circular ssDNA virus was identified. Genomic features and phylogenetic analysis showed that the virus belongs to the phylum Cressdnaviricota, which consists of viruses with circular, single-stranded DNA (ssDNA) genomes. Members of this phylum have been identified in various species and in environmental samples. The newly found virus is distantly related to the currently known members of the phylum Cressdnaviricota.


Subject(s)
Bivalvia/genetics , DNA Viruses/classification , DNA, Viral/genetics , Genome, Viral , Animals , DNA Viruses/isolation & purification , DNA, Circular/genetics , DNA, Single-Stranded/genetics , Environmental Microbiology , Metagenomics , Phylogeny , Sequence Analysis, DNA
2.
Am Surg ; 72(9): 833-6, 2006 Sep.
Article in English | MEDLINE | ID: mdl-16986396

ABSTRACT

Peripherally inserted central venous catheter (PICC) lines have become a frequently used method of intravenous access for long-term administration of antibiotics, chemotherapy, and parenteral nutrition. Catheter-related complications involving the arterial tree are rare. We report a case of a 25-year-old woman with a history of difficult PICC line placement that presented with an arteriovenous fistula in the left arm. Duplex ultrasound confirmed the diagnosis of a brachial artery-to-brachial vein arteriovenous fistula (AVF), and the patient underwent surgical repair. To our knowledge, this is the first reported case of an AVF resulting from PICC line placement. Correction of AVF is indicated to alleviate symptoms as well as to prevent future complications.


Subject(s)
Arteriovenous Fistula/etiology , Brachial Artery/abnormalities , Brachiocephalic Veins/abnormalities , Catheterization, Central Venous/adverse effects , Adult , Arteriovenous Fistula/diagnostic imaging , Brachial Artery/diagnostic imaging , Brachiocephalic Veins/diagnostic imaging , Female , Humans , Ultrasonography
3.
Am Surg ; 68(1): 65-9, 2002 Jan.
Article in English | MEDLINE | ID: mdl-12467321

ABSTRACT

Acute acalculous cholecystitis remains a diagnostic challenge in critically ill trauma patients. Laboratory studies are nonspecific and associated injuries or mental status changes may mask clinical signs and symptoms. We conducted a retrospective study to assess the utility of ultrasound in the diagnosis of acute acalculous cholecystitis. We hypothesized that ultrasound is inadequate as a screening tool for acute acalculous cholecystitis. The abdominal ultrasounds of all patients undergoing evaluation for acute acalculous cholecystitis in a 40-month period at our Level I trauma center were reviewed. Thickened gallbladder wall, pericholecystic fluid and emphysematous gallbladder were considered positive sonographic criteria. Sludge, cholelithiasis, and hydrops were considered suggestive. Patients who did not undergo cholecystectomy had their gallbladders evaluated either during subsequent laparotomy or at autopsy or they were discharged from the hospital without need for intervention. Sixty-two patients were included. Twenty-one patients underwent cholecystectomy for presumed acute acalculous cholecystitis. The data revealed a sensitivity of 30 per cent (6/20) and a specificity of 93 per cent (39/42) for ultrasound evaluation. Twenty patients had subsequent hepatobiliary scans [hepato-iminodiacetic acid (HIDA)] with a sensitivity of 100 per cent (12/12) and specificity of 88 per cent (7/8). Our data do not support ultrasound as a reliable routine screening tool for acute acalculous cholecystitis. Despite its convenience as a bedside procedure ultrasound has insufficient sensitivity to justify its use and a more sensitive diagnostic tool should be used.


Subject(s)
Cholecystitis/diagnostic imaging , Acute Disease , Adult , Cholecystectomy , Cholecystitis/epidemiology , Cholecystitis/surgery , Cholecystokinin , Comorbidity , Critical Illness , Humans , Imino Acids , Middle Aged , Radionuclide Imaging , Retrospective Studies , Ultrasonography , Wounds and Injuries/epidemiology
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