Subject(s)
Acetamides , Anti-Inflammatory Agents , Biphenyl Compounds , Acetamides/chemical synthesis , Acetamides/metabolism , Animals , Anti-Inflammatory Agents/chemical synthesis , Anti-Inflammatory Agents/metabolism , Biphenyl Compounds/chemical synthesis , Biphenyl Compounds/metabolism , Chemical Phenomena , Chemistry, Physical , Chromatography , Dogs , Humans , Rats , Spectrum AnalysisSubject(s)
Anti-Anxiety Agents/analysis , Animals , Anti-Anxiety Agents/metabolism , Benzodiazepines , Biotransformation , Chemical Phenomena , Chemistry , Dogs , RatsABSTRACT
A sensitive and specific assay, involving electron capture gas-liquid chromatography, has been developed for the identification of pinazepam and its metabolites in serum, urine and brain samples from dogs and rats after single or repeated oral administration of the drug. Serum and urine samples from healthy humans after a single oral administration have also been analysed. The identity of gas-liquid chromatographic peaks has been established by mass spectrometry. In blood serum and brain, only pinazepam and its N-depropargylated product (demethyldiazepam) were found; from urine, 3-hydroxypinazepam and oxazepam were also recovered. The sensitivity of the gas-liquid chromatographic method is of the order of 5-10 ng of pinazepam and 15-20 ng of the other three benzodiazepines per ml of serum.
