ABSTRACT
A strain of Pseudomonas fluorescens, capable of growing on styrene as the sole C source was isolated from enrichment cultures. From the Pseudomonas cultures supplied with styrene, phenylacetic and o-hydroxyphenylacetic acids were isolated and identified, o-Hydroxyphenylacetic acid was also isolated from the cultures supplied with phenylacetate. Homogentisate 1,2-dioxygenase is induced in the cells grown either on styrene or phenylacetate or o-hydroxyphenylacetate. A pathway for styrene metabolism through the intermediary formation of phenylacetate and o-hydroxyphenylacetate which is further oxidized via homogentisate is suggested and discussed.
ABSTRACT
1. Ring cleavage of 2,3-dihydroxybenzoate by cell-free extracts of Pseudomonas putida leads to 2-hydroxy-6-oxo-(2Z,4E)-hexa-2,4-dienoic acid and CO2. 2. The 1H n.m.r. spectrum of the ring-fission product obtained in a 2H2O solution suggests that the extra-diol cleavage occurs between C-3 and C-4.
Subject(s)
Hydroxybenzoates/metabolism , Pseudomonas/metabolism , Chemical Phenomena , Chemistry , Deuterium , Hydrogen , Magnetic Resonance SpectroscopyABSTRACT
Two Achromobacter strains capable of utilizing 2,2-dimethylheptane or tertbutylbenzene as the sole carbon and energy source were isolated from waste-water. Pivalic acid was found in the cultures of Achromobacter A1 containing 2,2-dimethylheptane. From cultures of Achromobacter A2 in the presence of tertbutylbenzene, a diol was isolated and identified as 2,3-dihydro-2,3-dihydroxytertbutylbenzene. Evidence for meta cleavage of the aromatic ring and for accumulation of pivalic acid in the cultures was also obtained. A metabolic pathway for tertbutylbenzene is suggested.
Subject(s)
Alcaligenes/metabolism , Benzene Derivatives/metabolism , Heptanes/metabolism , Water Microbiology , Biodegradation, Environmental , Chemical Phenomena , Chemistry , Pentanoic Acids/metabolism , SewageABSTRACT
1. 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid was isolated and identified from washed suspensions of Pseudomonas putida incubated in the presence of 2,3-dihydroxybiphenyl. 2. Benzoic acid was isolated from reaction mixtures of crude cell-free extracts incubated with 2,3-dihydroxybiphenyl. 3. The presence in the same reaction mixtures of either 4-hydroxy-2-oxovalerate or 2-hydroxypenta-2,4-dienoate was suggested by mass spectrometry. 4. The degradative pathway of biphenyl is discussed.
Subject(s)
Biphenyl Compounds/metabolism , Fatty Acids, Unsaturated/biosynthesis , Pseudomonas/metabolism , Benzoates/isolation & purification , Cell-Free System , Chromatography, Thin Layer , Keto Acids , Mass Spectrometry , Spectrophotometry, UltravioletABSTRACT
1. Two Pseudomonas strains capable of utilizing 2-phenylbutane, 3-phenylpentane and 4-phenylheptane as the sole carbon and energy source were isolated. 2. Two Nocardia strains capable of utilizing only 3-phenyldodecane as the sole carbon and energy source were isolated. 3. All the isolated strains were unable to grow on the corresponding phenylalkane-p-sulphonates. 4. From liquid cultures of Pseudomonas strains utilizing 2-phenylbutane, 2-(2,3-dihydro-2,3-dihydroxyphenyl)butane was isolated and identified. Evidence for a meta cleavage of the benzene ring was also obtained. 5. From liquid cultures of Pseudomonas strains utilizing 3-phenylpentane, 3-(2,3-dihydro-2,3-dihydroxyphenyl)pentane and 2-hydroxy-7-ethyl-6-oxonona-2,4-dienoic acid were isolated and identified. 6. Evidence for the formation of both a diol and a meta-cleavage compound was obtained from liquid cultures of both Pseudomonas strains utilizing 4-phenylheptane. 7. Liquid cultures of both Nocardia strains utilizing 3-phenyldodecane never formed a diol or a semialdehyde-related compound. 2-Phenylbutyric acid, 3-phenylvaleric acid and 4-phenylhexanoic acid were shown to be present in these cultures.