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Chest ; 113(2): 401-4, 1998 Feb.
Article in English | MEDLINE | ID: mdl-9498959

ABSTRACT

OBJECTIVE: This study determined which, if any, of the three superoxide dismutase (SOD) enzyme activities were detectable in BAL fluid (BALF). BACKGROUND: The identity and concentrations of antioxidant molecules in BALF have not been fully characterized. One important class of antioxidants is that of the SOD enzymes. METHODS: BALF from control nonsmokers (n=9), smokers (n=7), and asthmatic subjects (n=12) were examined for SOD activity by a modified pyrogallol auto-oxidation method. The particular SOD enzyme or enzymes responsible for any activity were identified based on activity inhibition data and gel filtration column chromatography patterns. RESULTS: SOD activity was detected in all samples. Unlike serum or some other fluids, in which the enzyme extracellular SOD accounts for virtually all SOD activity, the enzyme SOD 1 accounted for virtually all SOD activity. BALF SOD activities were lower for smokers than for control or asthmatic subjects (p<0.05). CONCLUSION: BALF SOD 1 activities can be measured as part of lung antioxidant studies. Data from a limited number of subjects suggested that smokers can have low BALF SOD values.


Subject(s)
Bronchoalveolar Lavage Fluid/chemistry , Superoxide Dismutase/analysis , Adult , Analysis of Variance , Antioxidants/analysis , Asthma/enzymology , Asthma/physiopathology , Bronchial Hyperreactivity/enzymology , Bronchoscopy , Chromatography, Gel , Cytosol/enzymology , Enzyme Inhibitors , Extracellular Space/enzymology , Female , Forced Expiratory Volume , Humans , Lung/enzymology , Male , Mitochondria/enzymology , Oxidation-Reduction , Pyrogallol/metabolism , Smoking/metabolism , Smoking/physiopathology , Superoxide Dismutase/antagonists & inhibitors , Superoxide Dismutase-1 , Vital Capacity
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