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1.
Mol Cell Neurosci ; 50(3-4): 238-49, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22687584

ABSTRACT

Here, we investigated an Immunoglobulin (Ig) superfamily protein IgSF8 which is abundantly expressed in olfactory sensory neuron (OSN) axons and their developing synapses. We demonstrate that expression of IgSF8 within synaptic neuropil is transitory, limited to the period of glomerular formation. Glomerular expression decreases after synaptic maturation and compartmental glomerular organization is achieved, although expression is maintained at high levels within the olfactory nerve layer (ONL). Immunoprecipitations indicate that IgSF8 interacts with tetraspanin CD9 in the olfactory bulb (OB). CD9 is a component of tetraspanin-enriched microdomains (TEMs), specialized microdomains of the plasma membrane known to regulate cell morphology, motility, invasion, fusion and signaling, in both the nervous and immune systems, as well as in tumors. In vitro, both IgSF8 and CD9 localize to puncta within axons and growth cones of OSNs, consistent with TEM localization. When the olfactory epithelium (OE) was lesioned, forcing OSN regeneration en masse, IgSF8 was once again able to be detected in OSN axon terminals as synapses were reestablished. Finally, we halted synaptic maturation within glomeruli by unilaterally blocking functional activity and found that IgSF8 did not undergo exclusion from this subcellular compartment and instead continued to be detected in adult glomeruli. These data support the hypothesis that IgSF8 facilitates OSN synapse formation.


Subject(s)
Axons/metabolism , Carrier Proteins/metabolism , Gene Expression Regulation, Developmental , Membrane Proteins/metabolism , Neural Cell Adhesion Molecules/metabolism , Olfactory Receptor Neurons/metabolism , Synapses/metabolism , Animals , Axons/chemistry , Carrier Proteins/analysis , Carrier Proteins/genetics , Female , Immunohistochemistry , Male , Membrane Proteins/analysis , Membrane Proteins/genetics , Mice , Mice, Inbred Strains , Neural Cell Adhesion Molecules/analysis , Neural Cell Adhesion Molecules/genetics , Olfactory Receptor Neurons/embryology , Olfactory Receptor Neurons/growth & development , Pregnancy , Tetraspanin 29/metabolism , Transcription, Genetic
2.
Front Psychiatry ; 2: 40, 2011.
Article in English | MEDLINE | ID: mdl-21747772

ABSTRACT

Regulation of maternal behavior in the immediate postpartum period involves neural circuits in reward and homeostasis systems responding to cues from the newborn. Our aim was to assess one specific regulatory mechanism: the role that olfaction plays in the onset and modulation of parenting behavior. We focused on changes in gene expression in olfactory brain regions, examining nine genes found in previous knockout studies to be necessary for maternal behavior. Using a quantitative PCR (qPCR)-based approach, we assessed changes in gene expression in response to exposure to pups in 11 microdissected olfactory brain regions. Over the first postpartum days, all nine genes were detected in all 11 regions (at differing levels) and their expression changed in response to pup exposure. As a general trend, five genes (Dbh, Esr1, FosB, Foxb1, and Oxtr) were found to decrease their expression in most of the olfactory regions examined, while two genes (Mest and Prlr) were found to increase expression. Nos1 and Peg3 levels remained relatively stable except in the accessory olfactory bulb (AOB), where greater than fourfold increases in expression were observed. The largest magnitude expression changes in this study were found in the AOB, which mediates a variety of olfactory cues that elicit stereotypic behaviors such as mating and aggression as well as some non-pheromone odors. Previous analyses of null mice for the nine genes assessed here have rarely examined olfactory function. Our data suggest that there may be olfactory effects in these null mice which contribute to the observed maternal behavioral phenotypes. Collectively, these data support the hypothesis that olfactory processing is an important sensory regulator of maternal behavior.

3.
J Comp Neurol ; 518(24): 4825-41, 2010 Dec 15.
Article in English | MEDLINE | ID: mdl-21031554

ABSTRACT

The embryonic development of the olfactory nerve includes the differentiation of cells within the olfactory placode, migration of cells into the mesenchyme from the placode, and extension of axons by the olfactory sensory neurons (OSNs). The coalition of both placode-derived migratory cells and OSN axons within the mesenchyme is collectively termed the "migratory mass." Here we address the sequence and coordination of the events that give rise to the migratory mass. Using neuronal and developmental markers, we show subpopulations of neurons emerging from the placode by embryonic day (E)10, a time at which the migratory mass is largely cellular and only a few isolated OSN axons are seen, prior to the first appearance of OSN axon fascicles at E11. These neurons also precede the emergence of the gonadotropin-releasing hormone neurons and ensheathing glia which are also resident in the mesenchyme as part of the migratory mass beginning at about E11. The data reported here begin to establish a spatiotemporal framework for the migration of molecularly heterogeneous placode-derived cells in the mesenchyme. The precocious emigration of the early arriving neurons in the mesenchyme suggests they may serve as "guidepost cells" that contribute to the establishment of a scaffold for the extension and coalescence of the OSN axons.


Subject(s)
Body Patterning/physiology , Cell Movement/physiology , Olfactory Mucosa/embryology , Olfactory Nerve/embryology , Olfactory Receptor Neurons/embryology , Animals , Axons/physiology , Mice , Mice, Transgenic , Neurons/physiology , Olfactory Mucosa/cytology , Olfactory Mucosa/physiology , Olfactory Nerve/cytology , Olfactory Nerve/physiology , Olfactory Receptor Neurons/cytology , Olfactory Receptor Neurons/physiology
4.
J Neurosci ; 30(30): 10067-75, 2010 Jul 28.
Article in English | MEDLINE | ID: mdl-20668191

ABSTRACT

As odorant receptors (ORs) are thought to be critical determinants of olfactory sensory neuron (OSN) axon targeting and organization, we examined the spatiotemporal onset of mice ORs expression from the differentiation of OSNs in the olfactory placode to an aging olfactory epithelium. ORs were first detected in the placode at embryonic day 9 (E9), at the onset of OSN differentiation but before axon extension. By E13, 22 of 23 ORs were expressed. Onset of individual OR expression was diverse; levels and patterns of expression were unique for each OR. Regional distribution of ORs within zones of the olfactory epithelium appeared stable across development; adult-like patterns were observed by E13. Finally, analysis of OR expression and chromosomal location suggests that ORs are not stochastically expressed; they show evidence of coordinated expression. Collectively, these studies demonstrate that ORs are not equally represented in the "olfactome" across an animal's lifespan.


Subject(s)
Gene Expression Regulation, Developmental/physiology , Olfactory Bulb/cytology , Olfactory Receptor Neurons/metabolism , Receptors, Odorant/metabolism , Algorithms , Animals , Animals, Newborn , Cell Differentiation/physiology , Chromosome Mapping/methods , Embryo, Mammalian , Mice , Mice, Inbred C57BL , Microarray Analysis/methods , Neural Cell Adhesion Molecules/metabolism , Olfactory Bulb/embryology , Olfactory Bulb/growth & development , Receptors, Odorant/genetics , Tubulin/metabolism
5.
Ann N Y Acad Sci ; 1170: 18-20, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19686100

ABSTRACT

Odorant receptors are thought to be critical determinants of olfactory sensory neuron axon targeting and organization. Nonetheless, a systematic characterization of the onset of odorant receptor expression has not yet been done in the main olfactory epithelium. Here, we briefly review our current understanding regarding the onset of odorant receptor expression in the main olfactory epithelium and identify some of those questions which we believe must be of high priority for future study.


Subject(s)
Receptors, Odorant/metabolism , Animals , Olfactory Mucosa/metabolism
6.
J Neurosci ; 29(30): 9405-16, 2009 Jul 29.
Article in English | MEDLINE | ID: mdl-19641104

ABSTRACT

We recently described the boundary-like expression pattern of the extracellular matrix molecule tenascin-C (Tnc) in the developing mouse olfactory bulb (OB) (Shay et al., 2008). In the present study, we test the hypothesis that Tnc inhibits olfactory sensory neuron (OSN) axon growth in the developing OB before glomerulogenesis. The period of time before glomerular formation begins, when axons remain restricted to the developing olfactory nerve layer (ONL), is crucial for axon sorting. Here, we show with in vitro analyses that OSN neurite outgrowth is inhibited by Tnc in a dose-dependent manner and that, in stripe assays, axons preferentially avoid Tnc. Using Tnc-null mice, we also show that that glomerular development is delayed in the absence of Tnc. In wild-type mice, OSN axons coalesce into immature or protoglomeruli, which further differentiate and segregate into glomeruli. Glomeruli are first identifiable as discrete structures at birth. In null mice, glomeruli appear immature at birth, remain fused to the ONL, and have a significantly larger diameter compared with wild-type controls. By postnatal day 4, null glomeruli are indistinguishable from controls. Thus, OSN axons appear delayed in their coalescence into glomerular structures. These data correlate with behavioral reports of Tnc-null mice, which are delayed by 24 h in their acquisition of an olfactory behavior (de Chevigny et al., 2006). Collectively, these data demonstrate that Tnc is an inhibitory boundary molecule in the developing OB during a key period of development.


Subject(s)
Axons/physiology , Olfactory Bulb/growth & development , Sensory Receptor Cells/physiology , Tenascin/metabolism , Animals , Animals, Newborn , In Vitro Techniques , Mice , Mice, Knockout , Neurites/physiology , Olfactory Bulb/physiology , Olfactory Mucosa/growth & development , Olfactory Mucosa/physiology , Receptor-Like Protein Tyrosine Phosphatases, Class 5/metabolism , Synapses/physiology , Tenascin/genetics
7.
Dev Dyn ; 237(7): 1837-50, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18570250

ABSTRACT

Olfactory sensory neuron (OSN) axons follow stereotypic spatio-temporal paths in the establishment of the olfactory pathway. Extracellular matrix (ECM) molecules are expressed early in the developing pathway and are proposed to have a role in its initial establishment. During later embryonic development, OSNs sort out and target specific glomeruli to form precise, complex topographic projections. We hypothesized that ECM cues may help to establish this complex topography. The aim of this study was to characterize expression of ECM molecules during the period of glomerulogenesis, when synaptic contacts are forming. We examined expression of laminin-1, perlecan, tenascin-C, and CSPGs and found a coordinated pattern of expression of these cues in the pathway. These appear to restrict axons to the pathway while promoting axon outgrowth within. Thus, ECM molecules are present in dynamic spatio-temporal positions to affect OSN axons as they navigate to the olfactory bulb and establish synapses.


Subject(s)
Extracellular Matrix Proteins/analysis , Extracellular Matrix Proteins/metabolism , Olfactory Pathways/metabolism , Animals , Female , Heparan Sulfate Proteoglycans/analysis , Heparan Sulfate Proteoglycans/metabolism , Immunohistochemistry , Laminin/analysis , Laminin/metabolism , Mice , Models, Biological , Olfactory Pathways/embryology , Pregnancy , Tenascin/analysis , Tenascin/metabolism
8.
Mol Cell Neurosci ; 32(1-2): 1-14, 2006.
Article in English | MEDLINE | ID: mdl-16531066

ABSTRACT

There is an overall topographic connectivity in the axonal projections of olfactory sensory neurons from the olfactory epithelium (OE) to the olfactory bulb (OB). The molecular determinants of this overall topographic OE-OB connectivity are not known. For 20 years, the intriguing expression pattern of the olfactory cell adhesion molecule (OCAM) has made it the leading candidate as determinant of overall topographic OE-OB connectivity. Here, we have generated a strain of OCAM knockout mice by gene targeting. There were no obvious alterations in the distribution of olfactory sensory neurons within the OE or in the coalescence of axons into specific glomeruli. However, the compartmental organization of dendrites and axons within the glomeruli was disrupted. Surprisingly, the mutant mice exhibited an increase in olfactory acuity; they appeared to have a better sense of smell. Thus, despite its striking expression pattern, OCAM is not essential for overall topographic OE-OB connectivity. Instead, OCAM is required for establishing or maintaining the compartmental organization and the segregation of axodendritic and dendrodendritic synapses within glomeruli.


Subject(s)
Axons/metabolism , Cell Differentiation/genetics , Dendrites/metabolism , Neural Cell Adhesion Molecules/genetics , Olfactory Bulb/abnormalities , Olfactory Receptor Neurons/abnormalities , Animals , Axons/ultrastructure , Cell Communication/genetics , Dendrites/ultrastructure , Female , Gene Expression Regulation, Developmental/genetics , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Mice, Transgenic , Neural Cell Adhesion Molecules/deficiency , Neuronal Plasticity/genetics , Neuropil/metabolism , Neuropil/ultrastructure , Olfactory Bulb/cytology , Olfactory Bulb/metabolism , Olfactory Nerve/abnormalities , Olfactory Nerve/cytology , Olfactory Nerve/metabolism , Olfactory Receptor Neurons/cytology , Olfactory Receptor Neurons/metabolism , Presynaptic Terminals/metabolism , Presynaptic Terminals/ultrastructure , Smell/genetics
9.
J Comp Neurol ; 482(2): 201-16, 2005 Feb 07.
Article in English | MEDLINE | ID: mdl-15611992

ABSTRACT

Neuron specific calcium sensor 1 (NCS-1) is widely expressed in the developing and adult nervous system. Like calmodulin, NCS-1 is a member of a family of calcium binding proteins that contain EF-hand motifs, which bind calcium and induce conformational changes in the protein. Their binding varies with calcium concentration, allowing them to act as true calcium sensors rather than just calcium binding proteins. This family of proteins has been implicated in important synaptic events including neurotransmitter release and synapse formation. We examined the expression of NCS-1 in the developing and mature olfactory system to determine whether this molecule may be playing a role in establishing and/or maintaining olfactory circuitry. During development, expression of NCS-1 in the olfactory epithelium was localized in the dendritic knobs and axons of olfactory sensory neurons. Axonal expression was down-regulated after synapse formation. In the developing olfactory bulb, NCS-1 was expressed in the processes of mitral/tufted and granule cells. However, in the adult olfactory bulb, strongest expression was found in a subset of periglomerular cells (PGCs). This subset of PGCs did not express other known markers of PGCs including tyrosine hydroxylase, glutamic acid decarboxylase, calbindin, or calretinin, and only partially overlapped with the subpopulation of PGCs that express parvalbumin. Together, these data suggest multiple and overlapping roles of NCS-1 in the developing and mature olfactory system.


Subject(s)
Calcium-Binding Proteins/metabolism , Neurons, Afferent/metabolism , Neuropeptides/metabolism , Olfactory Mucosa/metabolism , Olfactory Pathways/embryology , Olfactory Pathways/metabolism , Synapses/metabolism , Animals , Calcium-Binding Proteins/genetics , Female , Male , Mice , Mice, Transgenic , Neuronal Calcium-Sensor Proteins , Neurons, Afferent/ultrastructure , Neuropeptides/genetics , Olfactory Bulb/metabolism , Olfactory Bulb/ultrastructure , Olfactory Mucosa/embryology , Olfactory Mucosa/ultrastructure , Olfactory Pathways/ultrastructure , Synapses/ultrastructure
10.
J Comp Neurol ; 475(1): 128-41, 2004 Jul 12.
Article in English | MEDLINE | ID: mdl-15176089

ABSTRACT

The subventricular zone (SVZ) is a major neurogenic region in the adult brain. Cells from the SVZ give rise to two populations of olfactory bulb interneurons: the granule cells and periglomerular (PG) cells. Currently, little is known about the signaling pathways that direct these newly generated neurons to become either granule or PG neurons. In the present study, we used the nestin promoter and enhancer to direct expression of the tetracycline transactivator (tTA). We generated two independent strains of nestin-tTA transgenic animals and crossed founder mice from both lines to mice containing a tetracycline-regulated transgene (mCREB) whose expression served as a marker for the activity of the nestin-tTA transgene. mCREB expression occurred in a subset of proliferating cells in the SVZ and rostral migratory stream in both lines. Surprisingly, in both lines of nestin-tTA mice transgene expression in the olfactory bulb was limited to PG neurons and was absent from granule cells, suggesting that this nestin promoter construct differentiates between the two interneuronal populations. Transgene expression occurred in several subtypes of PG neurons, including those expressing calretinin, calbindin, GAD67, and tyrosine hydroxylase. These results suggest that a unique subset of SVZ precursor cells gives rise to PG, and not granule cells. The ability to express different transgenes within this subpopulation of neuronal precursors provides a powerful system to define the signals regulating the differentiation and survival of adult-generated neurons in the olfactory bulb.


Subject(s)
Intermediate Filament Proteins/genetics , Nerve Tissue Proteins/genetics , Neurons/metabolism , Olfactory Bulb/cytology , Promoter Regions, Genetic/physiology , Stem Cells/physiology , Transgenes/physiology , Animals , Cyclic AMP Response Element-Binding Protein/biosynthesis , Cyclic AMP Response Element-Binding Protein/genetics , Gene Expression Regulation/physiology , Intermediate Filament Proteins/biosynthesis , Intermediate Filament Proteins/physiology , Mice , Mice, Transgenic , Nerve Tissue Proteins/biosynthesis , Nerve Tissue Proteins/physiology , Nestin , Neurons/cytology , Olfactory Bulb/metabolism , Stem Cells/metabolism
11.
J Comp Neurol ; 467(1): 22-31, 2003 Dec 01.
Article in English | MEDLINE | ID: mdl-14574677

ABSTRACT

Cell surface carbohydrates have been implicated in axon guidance and targeting throughout the nervous system. We have begun to test the hypothesis that, in the olfactory system, a differential distribution of cell surface carbohydrates may influence olfactory sensory neuron (OSN) axon targeting. Specifically, we have examined the spatial distribution of two different plant lectins, Ulex europaeus agglutinin (UEA) and Dolichos biflorus agglutinin (DBA), to determine whether they exhibit differential and reproducible projections onto the main olfactory bulb. Each lectin exhibited a unique spatial domain of glomerular labeling that was consistent across animals. UEA labeling was strongest in the ventral aspect of the olfactory bulb; DBA labeling was strongest in the dorsal aspect of the olfactory bulb. Some evidence for colocalization was present where these two borders intersected. Large areas of the glomerular layer were not labeled by either lectin. To determine whether patterns of lectin labeling were reproducible at the level of individual glomeruli, UEA labeling was assessed relative to M72-IRES-taulacZ- and P2-IRES-taulacZ-labeled axons. Although glomeruli neighboring these two identified glomeruli were consistently labeled with UEA, none of the lacZ positive axons was lectin labeled. Labeling of vomeronasal sensory neuron axons in the accessory olfactory bulb was more uniform for the two lectins. These data are the first to show a differential distribution of UEA vs. DBA labeling in the main olfactory bulb and are consistent with the hypothesis that a differential distribution of cell surface carbohydrates, a glycocode, may contribute to the targeting of OSN axons.


Subject(s)
Olfactory Bulb/growth & development , Olfactory Receptor Neurons/chemistry , Olfactory Receptor Neurons/growth & development , Plant Lectins , Animals , Axons , Cell Membrane , Female , Immunohistochemistry , Mice , Mice, Inbred C57BL , Neurons, Afferent/chemistry , Olfactory Bulb/chemistry , Olfactory Mucosa/chemistry , Olfactory Pathways/growth & development , Plant Lectins/analysis , Vomeronasal Organ/growth & development
12.
J Comp Neurol ; 458(4): 389-403, 2003 Apr 14.
Article in English | MEDLINE | ID: mdl-12619073

ABSTRACT

The projection of olfactory sensory neuron (OSN) axons from the olfactory epithelium (OE) to the olfactory bulb (OB) is highly organized but topographically complex. Evidence suggests that odorant receptor expression zones in the OE map to the OB about orthogonal axes. One candidate molecule for the formation of zone-specific targeting of OSN axon synapses onto the OB is the olfactory cell adhesion molecule (OCAM). OCAM(+) OSNs are restricted to three of the four zones in the OE and project their axons to the ventral OB where they form synapses with mitral/tufted (M/T) cells. To determine when this zonal connection is established, we have examined OCAM expression in rat olfactory system, during seminal periods of glomerular formation. OCAM(+) axons sort out in the ventral olfactory nerve layer of the OB before glomerular formation. Surprisingly, OCAM was also expressed transiently by subsets of M/T cell dendrites located in the dorsal OB. The expression of OCAM by OSN axons and M/T dendrites was asymmetrical; in the dorsal OB, OCAM(-) OSN axons synapsed on OCAM(+) M/T dendrites, whereas in the ventral OB, OCAM(+) OSN axons synapsed on OCAM(-) M/T dendrites. The restricted spatial map of OCAM(+) M/T cells appeared earlier in development than the zonal segregation of OCAM(+) OSN axons. Thus, OCAM on M/T cell dendrites may act in a spatiotemporal window to specify regions of the developing rat OB, thereby establishing a foundation for mapping of the OE zonal organization onto the OB.


Subject(s)
Axons/metabolism , Dendrites/metabolism , Neural Cell Adhesion Molecules/biosynthesis , Olfactory Bulb/embryology , Olfactory Bulb/growth & development , Animals , Animals, Newborn , Axons/ultrastructure , Cells, Cultured , Dendrites/ultrastructure , Embryo, Mammalian , Immunohistochemistry , Microscopy, Confocal , Microscopy, Electron , Olfactory Bulb/metabolism , Olfactory Bulb/ultrastructure , Rats , Rats, Sprague-Dawley
13.
Cell Tissue Res ; 308(1): 7-17, 2002 Apr.
Article in English | MEDLINE | ID: mdl-12012202

ABSTRACT

Cell surface carbohydrates, both in the olfactory system and elsewhere, have been proposed to play critical roles in axon guidance and targeting. Recent studies have used plant lectins to study the heterogeneous distribution of carbohydrates in the olfactory system. One lectin, Dolichos biflorus agglutinin (DBA), heterogeneously labels subsets of glomeruli. In the olfactory epithelium DBA labeled a subset of olfactory sensory neurons (OSNs) including their cilia, dendrites, and somata. OSN axons were also labeled and readily observed in the olfactory nerve and bulb. The patterns of glomerular innervation by DBA labeled (DBA(+)) axons were diverse; some glomeruli contained many labeled axons, while others contained few or no labeled axons. To characterize the heterogeneous innervation of glomeruli, we double labeled olfactory bulbs with DBA and an antibody to olfactory marker protein (OMP). OMP colocalized in most, but not all, DBA(+) axons. To determine if those axons that did not express OMP were immature, we double labeled olfactory bulbs with DBA and anti-GAP-43. GAP-43 rarely colocalized with DBA, suggesting that DBA(+) axons are not, as a population, immature. Triple labeling with all three markers revealed a small subset of DBA(+) axons which did not express either OMP or GAP-43. Electron microscopy established that DBA labels axons in the olfactory nerve and DBA-labeled axons form typical glomerular axodendritic synapses.


Subject(s)
Axons/chemistry , Carbohydrates/analysis , Olfactory Bulb/chemistry , Plant Lectins , Receptors, Odorant/chemistry , Sensory Receptor Cells/chemistry , Animals , Axons/ultrastructure , Cell Membrane/chemistry , GAP-43 Protein/analysis , GAP-43 Protein/metabolism , Immunohistochemistry , Lectins/metabolism , Mice , Mice, Inbred C57BL , Nerve Tissue Proteins/analysis , Nerve Tissue Proteins/metabolism , Olfactory Bulb/cytology , Olfactory Bulb/metabolism , Olfactory Bulb/ultrastructure , Olfactory Marker Protein , Olfactory Mucosa/innervation , Olfactory Mucosa/ultrastructure , Receptors, Odorant/metabolism , Sensory Receptor Cells/cytology , Sensory Receptor Cells/metabolism , Synapses/ultrastructure
14.
J Comp Neurol ; 446(1): 68-80, 2002 Apr 22.
Article in English | MEDLINE | ID: mdl-11920721

ABSTRACT

Olfactory sensory neuron (OSN) axons coalesce to form the olfactory nerve (ON) and then grow from the olfactory epithelium to the olfactory bulb (OB), enter the olfactory nerve layer (ONL), reorganize extensively, and innervate specific glomeruli. Within the ON and ONL a population of glial cells, the olfactory ensheathing cells (OECs), surround OSN axon fascicles. To better understand the relationship between OECs and axon fascicles in the ONL of the adult mouse, we used confocal microscopy and antibodies to the low affinity nerve growth factor receptor p75 (p75), glial fibrillary acidic protein (GFAP), neuropeptide Y (NPY), and S-100 to identify glia. Antibodies to olfactory marker protein (OMP) and neuronal cell adhesion molecule (NCAM) were used to identify OSN axons. Electron microscopy characterized the ONL ultrastructure. We found that glial processes were not uniformly distributed in the ONL of the mouse. The p75(+) OEC processes were restricted to the ON and the outer ONL sublamina, and oriented parallel to the plane of the OB layers. In the inner ONL NPY(+) OEC-like processes were seen. GFAP(+) processes were restricted to the inner ONL sublamina, the ONL/GL boundary, and the GL, where they delineated loosely aggregated axon fascicles that entered the glomeruli obliquely. S-100(+) processes and somata were distributed throughout the ONL; the outer and inner ONL were equivalent in their S-100 staining. Ultrastructural studies showed that, although OECs could be identified in both the outer and inner ONL, in the latter, their relationship to bundles of OEC axons appeared less orderly than seen in the outer ONL. Our data demonstrate a differential organization of the ONL that could subserve distinct functions; axon extension may occur predominantly in the outermost ONL, whereas glomerular targeting occurs in the inner sublamina of the ONL.


Subject(s)
Axons/ultrastructure , Neuroglia/ultrastructure , Neuropil/ultrastructure , Olfactory Bulb/ultrastructure , Synapses/ultrastructure , Animals , Axons/metabolism , Fluorescent Antibody Technique , Glial Fibrillary Acidic Protein/metabolism , Immunohistochemistry , Mice , Mice, Inbred Strains , Microscopy, Electron , Neural Cell Adhesion Molecules/metabolism , Neuroglia/metabolism , Neuropeptide Y/metabolism , Neuropil/metabolism , Olfactory Bulb/metabolism , Receptor, Nerve Growth Factor/metabolism , S100 Proteins/metabolism , Synapses/metabolism
15.
J Neurosci ; 22(7): 2469-77, 2002 Apr 01.
Article in English | MEDLINE | ID: mdl-11923411

ABSTRACT

Axons from olfactory sensory neurons (OSNs) expressing a specific odorant receptor (OR) project to specific subsets of glomeruli in the olfactory bulb (for review, see Mombaerts, 1999, 2001). The aim of this study was to examine the trajectories that subsets of axons from OSNs expressing the same OR follow within the olfactory nerve and olfactory nerve layer (ONL) of adult mice. Using confocal microscopy, we generated serial reconstructions of axons from M72-IRES-tauGFP-expressing OSNs as they coursed within the ONL and into glomeruli. GFP-expressing axons were loosely aggregated in the outer ONL; however, as they entered the inner ONL, the majority fasciculated with other GFP-expressing axons before entering the glomerular neuropil. Although the vast majority of axons entered the glomerulus from the directly apposed ONL, some followed tortuous courses through and/or around adjacent glomeruli before terminating in the target glomerulus. Similar observations were made on subpopulations of axons in M71-IRES-tauGFP and P2-IRES-tauGFP mice. Ultrastructural analyses of labeled M72 glomeruli showed no evidence of axodendritic synapses other than those with GFP-labeled axon terminals. These data are consistent with the notion that OSN axons are highly precise in targeting glomeruli and that glomeruli, in turn, are highly homogeneous with regard to the OR expressed by the innervating OSNs. Because some single axons could follow idiosyncratic trajectories to the target glomerulus, it appears that stable homotypic fasciculation is not a prerequisite for correct targeting.


Subject(s)
Axons/ultrastructure , Olfactory Bulb/cytology , Olfactory Receptor Neurons/cytology , Animals , Axons/physiology , Green Fluorescent Proteins , Immunohistochemistry , Luminescent Proteins/genetics , Mice , Mice, Transgenic , Microscopy, Electron , Olfactory Bulb/metabolism , Olfactory Bulb/ultrastructure , Olfactory Receptor Neurons/metabolism , Olfactory Receptor Neurons/ultrastructure , Receptors, Odorant/biosynthesis , Receptors, Odorant/genetics , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/genetics
16.
J Neurosci ; 22(3): 766-74, 2002 Feb 01.
Article in English | MEDLINE | ID: mdl-11826106

ABSTRACT

The murine olfactory system consists of two primary divisions: (1) a main olfactory system, in which olfactory sensory neurons (OSNs) located in the main olfactory epithelium (MOE) send their axons to glomeruli in the main olfactory bulb (MOB); and (2) an accessory olfactory system, in which OSNs located in the vomeronasal organ send their axons to glomeruli in the accessory olfactory bulb (AOB). In labeling studies using the lectin Ulex europaeus agglutinin (UEA), we discovered a novel subset of small neuropilar structures in the MOB that are distinct from other glomeruli both in the MOB and AOB. These "microglomeruli" are morphologically similar to MOB glomeruli in many respects: they receive innervation from processes present in the olfactory nerve layer and are isolated from other glomeruli by juxtaglomerular cells; in addition, the compartmental pattern of UEA labeling suggests the presence of UEA (-) processes within their neuropil. Microglomeruli contained processes that express the olfactory marker protein, a marker common to mature OSN axons. However, unlike other glomerular structures, the microglomeruli did not contain neural cell adhesion molecule-labeled processes. Within microglomeruli, UEA(+) processes interdigitated with MAP2(+) dendrites, some of which likely originate from interneurons, as indicated by glutamic acid decarboxylase labeling. Synaptophysin labeling in microglomeruli strongly suggested that synapses occur between UEA(+) processes and dendrites. Anterograde labeling of OSNs, by injection of rhodamine-dextran into one naris, demonstrated that UEA(+) processes in microglomeruli originated in the MOE. The unique morphology, protein expression, and location of microglomeruli have led us to hypothesize that they represent a novel class of glomerular structures in the murine olfactory system.


Subject(s)
Neuropil/ultrastructure , Olfactory Bulb/ultrastructure , Plant Lectins , Animals , Animals, Outbred Strains , Antigens, Differentiation/biosynthesis , Axons/ultrastructure , Dendrites/ultrastructure , Female , Immunohistochemistry , Lectins , Mice , Nerve Tissue Proteins/biosynthesis , Neural Cell Adhesion Molecules/metabolism , Neuropil/metabolism , Olfactory Bulb/metabolism , Olfactory Marker Protein , Synapses/ultrastructure
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