New perspectives for neutron imaging through advanced event-mode data acquisition.

Imaging using scintillators is a widespread and cost-effective approach in radiography. While different types of scintillator and sensor configurations exist, it can be stated that the detection efficiency and resolution of a scintillator-based system strongly depend on the scintillator material and its thickness. Recently developed event-driven detectors are capable of registering spots of light emitted by the scintillator after a particle interaction, allowing to reconstruct the Center-of-Mass of the interaction within the scintillator. This results in a more precise location of the event and therefore provides a pathway to overcome the scintillator thickness limitation and increase the effective spatial resolution of the system. Utilizing this principle, we present a detector capable of Time-of-Flight imaging with an adjustable field-of-view, ad-hoc binning and re-binning of data based on the requirements of the experiment including the possibility of particle discrimination via the analysis of the event shape in space and time. It is considered that this novel concept might replace regular cameras in neutron imaging detectors as it provides superior detection capabilities with the most recent results providing an increase by a factor 3 in image resolution and an increase by up to a factor of 7.5 in signal-to-noise for thermal neutron imaging.

Time-resolved RIXS experiment with pulse-by-pulse parallel readout data collection using X-ray free electron laser.

Time-resolved resonant inelastic X-ray scattering (RIXS) is one of the developing techniques enabled by the advent of X-ray free electron laser (FEL). It is important to evaluate how the FEL jitter, which is inherent in the self-amplified spontaneous emission process, influences the RIXS measurement. Here, we use a microchannel plate (MCP) based Timepix soft X-ray detector to conduct a time-resolved RIXS measurement at the Ti L3-edge on a charge-density-wave material TiSe2. The fast parallel Timepix readout and single photon sensitivity enable pulse-by-pulse data acquisition and analysis. Due to the FEL jitter, low detection efficiency of spectrometer, and low quantum yield of RIXS process, we find that less than 2% of the X-ray FEL pulses produce signals, preventing acquiring sufficient data statistics while maintaining temporal and energy resolution in this measurement. These limitations can be mitigated by using future X-ray FELs with high repetition rates, approaching MHz such as the European XFEL in Germany and LCLS-II in the USA, as well as by utilizing advanced detectors, such as the prototype used in this study.

Energy-resolved neutron imaging options at a small angle neutron scattering instrument at the Australian Center for Neutron Scattering.

Energy-resolved neutron imaging experiments conducted on the Small Angle Neutron Scattering (SANS) instrument, Bilby, demonstrate how the capabilities of this instrument can be enhanced by a relatively simple addition of a compact neutron counting detector. Together with possible SANS sample surveying and location of the region of interest, this instrument is attractive for many imaging applications. In particular, the combination of the cold spectrum of the neutron beam and its pulsed nature enables unique non-destructive studies of the internal structure for samples that are opaque to other more traditional techniques. In addition to conventional white beam neutron radiography, we conducted energy-resolved imaging experiments capable of resolving features related to microstructure in crystalline materials with a spatial resolution down to ∼0.1 mm. The optimized settings for the beamline configuration were determined for the imaging modality, where the compromise between the beam intensity and the achievable spatial resolution is of key concern.

Design and Characterisation of Metallic Glassy Alloys of High Neutron Shielding Capability.

This paper reports the design, making and characterisation of a series of Fe-based bulk metallic glass alloys with the aim of achieving the combined properties of high neutron absorption capability and sufficient glass forming ability. Synchrotron X-ray diffraction and pair distribution function methods were used to characterise the crystalline or amorphous states of the samples. Neutron transmission and macroscopic attenuation coefficients of the designed alloys were measured using energy resolved neutron imaging method and the very recently developed microchannel plate detector. The study found that the newly designed alloy (Fe48Cr15Mo14C15B6Gd2 with a glass forming ability of Ø5.8 mm) has the highest neutron absorption capability among all Fe-based bulk metallic glasses so far reported. It is a promising material for neutron shielding applications.

Single-quantum dot imaging with a photon counting camera.

The expanding spectrum of applications of single-molecule fluorescence imaging ranges from fundamental in vitro studies of biomolecular activity to tracking of receptors in live cells. The success of these assays has relied on progress in organic and non-organic fluorescent probe developments as well as improvements in the sensitivity of light detectors. We describe a new type of detector developed with the specific goal of ultra-sensitive single-molecule imaging. It is a wide-field, photon-counting detector providing high temporal and high spatial resolution information for each incoming photon. It can be used as a standard low-light level camera, but also allows access to a lot more information, such as fluorescence lifetime and spatio-temporal correlations. We illustrate the single-molecule imaging performance of our current prototype using quantum dots and discuss on-going and future developments of this detector.

Microchannel plate cross-strip detectors with high spatial and temporal resolution.

The cross-strip imaging readout employs charge division, and centroiding, of microchannel plate charge signals detected on two orthogonal layers of sense strips to encode event X-Y positions and times. We have developed cross-strip detectors and fully parallel channel position encoding electronics. The front-end amplifiers utilize two 32-channel pre-amplifier ASICs that send signals to a full 64-channel 60 MHz ADC circuit followed by a FPGA event-processing board. Tests with a software Finite Impulse Response filter and centroiding algorithm demonstrate <10mm resolution with a 32mm cross-strip anode detector using low microchannel plate gain (∼10(6)). The self-triggered event timing accuracy is 750 ps, and the system is capable of encoding photons at >1 MHz in combination with firmware-based FPGA centroiding algorithms.

Phasor-based single-molecule fluorescence lifetime imaging using a wide-field photon-counting detector.

Fluorescence lifetime imaging (FLIM) is a powerful approach to studying the immediate environment of molecules. For example, it is used in biology to study changes in the chemical environment, or to study binding processes, aggregation, and conformational changes by measuring Förster resonance energy transfer (FRET) between donor and acceptor fluorophores. FLIM can be acquired by time-domain measurements (time-correlated single-photon counting) or frequency-domain measurements (with PMT modulation or digital frequency domain acquisition) in a confocal setup, or with wide-field systems (using time-gated cameras). In the best cases, the resulting data is analyzed in terms of multicomponent fluorescence lifetime decays with demanding requirements in terms of signal level (and therefore limited frame rate). Recently, the phasor approach has been proposed as a powerful alternative for fluorescence lifetime analysis of FLIM, ensemble, and single-molecule experiments. Here we discuss the advantages of combining phasor analysis with a new type of FLIM acquisition hardware presented previously, consisting of a high temporal and spatial resolution wide-field single-photon counting device (the H33D detector). Experimental data with live cells and quantum dots will be presented as an illustration of this new approach.

Electronic and optical moi? Interference with microchannel plates: artifacts and benefits.

The spatial resolution of position-sensitive detectors that use stacks of microchannel plates (MCP's) with high-resolution anodes can be better than 20-microm FWHM [Proc. SPIE 3114, 283-294 (1997)]. At this level of accuracy, channel misalignments of the MCP's in the stack can cause observable moiré interference patterns. We show that the flat-field detector response can have moiré beat pattern modulations of as great as approximately 27% with periods from as small as a few channel diameters to as great as the size of a MCP multifiber. These modulations, however, may be essentially eliminated by rotation of the MCP's or by a mismatch of the channel sizes. We also discuss how the modulation phenomena can be a useful tool for mapping the metric nonlinearities of MCP detector readout systems. Employing the optical moiré effect, we demonstrate a simple, but effective, technique for evaluation of geometrical deformations simultaneously over a large MCP area. For a typical MCP, with a 60-channel-wide multifiber, we can obtain accuracies of 1.2 mrad for multifiber rotations and twists and 35/(L/p) mrad for channel-long axis distortions (where L/p is MCP thickness to interchannel distance ratio). This technique may be used for the development of MCP x-ray optics, which impose tight limitations on geometrical distortions, which in turn are not otherwise easily measurable with high accuracy.