Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
2.
Genetika ; 39(3): 341-8, 2003 Mar.
Article in Russian | MEDLINE | ID: mdl-12722633

ABSTRACT

The sbr gene of Drosophila melanogaster belongs to the NXF (nuclear export factor) family responsible for the mRNA transport from nucleus to cytoplasm. We have shown that in the heat-exposed (37 degrees C, 1 h) females, the l(1)ts403 (sbr10) mutation leads, in particular, to the high-frequency nondisjunction and loss of sex chromosomes in meiosis. For this trait, the incomplete dominance of the sbr10 mutation is observed. At the same time, the sbr10 mutation is recessive for many other traits of the heat-exposed flies: reduced viability, low fertility, impaired synthesis of the heat shock proteins, etc. The females heterozygous for the null allele (Df(1)vL4, a deletion eliminating gene srb) do not differ from females homozygous for the wild-type allele in frequency of the heat shock-induced nondisjunction and loss of sex chromosomes in meiosis. Because of this, the sbr10 mutation can be assigned to the gain-of-function alleles (those gaining the dominance function). Expression of the mutant sbr10 allele against the background of the wild-type allele suggests that in the heat shock-exposed females, the heat-modified product of this ts allele has a strong effect on sex chromosome disjunction in meiosis.


Subject(s)
Drosophila melanogaster/genetics , Meiosis , Mutation , Nondisjunction, Genetic , Sex Chromosomes/genetics , Animals , Drosophila Proteins/genetics , Female , Fertility , Genes, Dominant , Heat-Shock Response/genetics , Hot Temperature , Loss of Heterozygosity , Nuclear Proteins/genetics , RNA-Binding Proteins/genetics
3.
Genetika ; 37(6): 725-36, 2001 Jun.
Article in Russian | MEDLINE | ID: mdl-11517758

ABSTRACT

A DNA sequence from the 9F region of Drosophila melanogaster polytene chromosomes was cloned. Sequencing the cloned region and its comparison with the known sequences of the D. melanogaster genome showed that the cloned DNA part contains gene sbr and adjacent sequences. The literature data on the structure and functions of genes TAP in humans and Mex67 in yeast are discussed. These genes are orthologous to the sbr gene of Drosophila and control mRNA export from the nucleus to the cytoplasm. The literature evidence is consistent with the recessive expression of mutation l(1)ts403 (sbr10) upon heat treatment that is manifested as impaired HSP synthesis at the posttranscriptional level. However, it fails to explain the semidominant effect of the mutation manifested in high frequency of meiotic sex-chromosome nondisjunction in heat-treated females. A comparison of amino-acid sequences corresponding to the products of the three orthologous genes, TAP, Mex67, and sbr, showed that the sbr gene product of Drosophila is more similar to the human TAP factor than to the Mex67 factor in yeast.


Subject(s)
Drosophila melanogaster/genetics , Genes, Insect , Nuclear Proteins/genetics , Nucleocytoplasmic Transport Proteins , RNA-Binding Proteins/genetics , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , ATP Binding Cassette Transporter, Subfamily B, Member 2 , ATP-Binding Cassette Transporters , Amino Acid Sequence , Animals , Cloning, Molecular , DNA, Complementary/analysis , DNA, Complementary/genetics , Genome , Genome, Human , Humans , Molecular Sequence Data , Mutation , Sequence Alignment , Sequence Analysis
SELECTION OF CITATIONS
SEARCH DETAIL
...