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1.
Appl Opt ; 61(1): 135-140, 2022 Jan 01.
Article in English | MEDLINE | ID: mdl-35200812

ABSTRACT

The transmission properties of a photonic crystal immersed in several different oils have been characterized using terahertz time domain spectroscopy in the spectral range of 0.3-1.5 THz. As in previous works, oil samples can be distinguished using terahertz transmission measurements. When the same oils are introduced into a photonic crystal, we find that the effective refractive index of the photonic crystal is sensitive to the properties of the oils and shows differences not seen in bulk measurements. These effects are described in detail and have potential applications in both the sensing of very small volumes of oils and in the fine control of the refractive indices of photonic crystals.

2.
Opt Express ; 29(12): 19120-19125, 2021 Jun 07.
Article in English | MEDLINE | ID: mdl-34154153

ABSTRACT

We have developed a continuous wave sub-wavelength terahertz (THz) imaging system that combines two prominent classical optical techniques: solid immersion microscopy and interferometric detection. This combination allows for resolution beyond the diffraction limit at 703 GHz. We experimentally demonstrate sub-wavelength spatial resolution working with a relatively low-cost pyroelectric detector and with both high and low contrast samples.

3.
Biomed Opt Express ; 11(3): 1432-1448, 2020 Mar 01.
Article in English | MEDLINE | ID: mdl-32206420

ABSTRACT

The vital molecule serotonin modulates the functioning of the nervous system. The chemical characteristics of serotonin provide multiple advantages for its study in living or fixed tissue. Serotonin has the capacity to emit fluorescence directly and indirectly through chemical intermediates in response to mono- and multiphoton excitation. However, the fluorescent emissions are multifactorial and their dependence on the concentration, excitation wavelength and laser intensity still need a comprehensive study. Here we studied the fluorescence of serotonin excited multiphotonically with near-infrared light. Experiments were conducted in a custom-made multiphoton microscope coupled to a monochromator and a photomultiplier that collected the emissions. We show that the responses of serotonin to multiphoton stimulation are highly non-linear. The well-known violet emission having a 340 nm peak was accompanied by two other emissions in the visible spectrum. The best excitor wavelength to produce both emissions was 700 nm. A green emission with a ∼ 500 nm peak was similar to a previously described fluorescence in response to longer excitation wavelengths. A new blue emission with a ∼ 405 nm peak was originated from the photoconversion of serotonin to a relatively stable product. Such a reaction could be reproduced by irradiation of serotonin with high laser power for 30 minutes. The absorbance of the new compound expanded from ∼ 315 to ∼ 360 nm. Excitation of the irradiated solution monophotonically with 350 nm or biphotonically with 700 nm similarly generated the 405 nm blue emission. Our data are presented quantitatively through the design of a single geometric chart that combines the intensity of each emission in response to the serotonin concentration, excitation wavelengths and laser intensity. The autofluorescence of serotonin in addition to the formation of the two compounds emitting in the visible spectrum provides diverse possibilities for the quantitative study of the dynamics of serotonin in living tissue.

4.
J Biophotonics ; 11(8): e201700235, 2018 08.
Article in English | MEDLINE | ID: mdl-29498484

ABSTRACT

The gradual process in which the crystalline lens is cloudy due to the appearance of elements giving rise to variations in the refractive index is known as cataract. Clinical assessment is usually complicated because it considers patient's perception, and individuals with similar development have different visual deficits. This work presents a model which considers the fluctuations in the refractive index as spherical particles produce measurable scatter radial profiles patterns on the retina. Measurements for 2 different wavelengths simultaneously provide information on particle size and a quantitative assessment by measurement of the fluctuations of the refractive index.


Subject(s)
Cataract/physiopathology , Eye/physiopathology , Eye/radiation effects , Scattering, Radiation , Humans , Models, Biological , Refractometry , Retina/physiopathology , Retina/radiation effects
5.
Opt Express ; 15(5): 2517-29, 2007 Mar 05.
Article in English | MEDLINE | ID: mdl-19532488

ABSTRACT

In this paper the response purely refractive of a thin nonlinear material, in the z-scan technique experiment, is modeled as a lens with a focal length that is a function of some integer power of the incident beam radius. We demonstrate that different functional dependences of the photoinduced lens of a thin nonlinear material give typical z-scan curves with special features. The analysis is based on the propagation of Gaussian beams in the approximation of thin lens and small distortion for the nonlinear sample. We obtain that the position of the peak and valley, the transmittance near the focus and the transmittance far from the Rayleigh range depend on the functional dependence of the focal length. Special values of the power reproduce the results obtained for some materials under cw excitation.

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