ABSTRACT
A new sutureless technique to successfully anastomose the abdominal aorta of rats (1.3 mm in diameter) by using a fully biodegradable, laser-activated protein solder is presented. A total of 90 rats were divided into two groups randomly. In group one, the anastomoses were performed by using conventional microsuturing technique, whereas in group two, the anastomoses were performed by using a new laser welding technique. In addition, each of the two groups were divided into five subgroups and evaluated at different follow-up periods (10 minutes, 1 hour, 1 day, 1 week, and 6 weeks). At these intervals, the anastomoses were evaluated for patency and tensile strength. Three anastomoses in each subgroup were processed for light and electron microscopy. All anastomoses were found to be patent. The mean clamp time of the anastomoses performed with conventional suturing was 20.6 minutes compared with 7.2 minutes for the laser-activated welded anastomoses (p < 0.001). The strain measurements showed a stronger mechanical bond of the sutured anastomoses in the initial phase. However, at 6 weeks the tensile strength of the laser-welded anastomoses was higher compared with the conventional suture technique. Histologic evaluations revealed a near complete resorption of the solder after 6 weeks. The junction site of the vessel ends cannot be determined on the luminal side of the artery. In conclusion, a resorbable protein used as a solder, activated by a diode laser, can provide a reliable, safe, and rapid arterial anastomosis, which could be performed by any microsurgeon faster than conventional suturing after a short learning curve.
Subject(s)
Anastomosis, Surgical/instrumentation , Arteries/surgery , Lasers , Microsurgery/instrumentation , Serum Albumin, Bovine , Suture Techniques/instrumentation , Tissue Adhesives , Welding/instrumentation , Animals , Aorta, Abdominal/pathology , Aorta, Abdominal/surgery , Arteries/pathology , Biodegradation, Environmental , Male , Microscopy, Electron , Microscopy, Electron, Scanning , Rats , Rats, Wistar , Wound Healing/physiologyABSTRACT
A reliable method of exposure of the inferior alveolar nerve in Wistar rats has been developed, to allow intraosseous repair with two microsurgical techniques under halothane inhalational anaesthesia. The microsuturing technique involves anastomosis with 10-0 nylon sutures; a laser-weld technique uses an albumin-based solder containing indocyanine green, plus an infrared (810 nm wavelength) diode laser Seven animals had left inferior alveolar nerve repairs performed with the microsuture and laser-weld techniques. Controls were provided by unoperated nerves in the repaired cases. Histochemical analysis was performed utilizing neuron counts and horseradish peroxidase tracer (HRP) uptake in the mandibular division of the trigeminal ganglion, following sacrifice and staining of frozen sections with cresyl violet and diaminobenzidene. The results of this analysis showed similar mean neuron counts and mean HRP uptake by neurons for the unoperated controls and both microsuture and laser-weld groups. This new technique of intraosseous exposure of the inferior alveolar nerve in rats is described. It allows reliable and reproducible microsurgical repairs using both microsuture and laser-weld techniques.
Subject(s)
Laser Therapy/methods , Mandibular Nerve/surgery , Microsurgery/methods , Animals , Benzoxazines , Coloring Agents , Disease Models, Animal , Histocytochemistry , Horseradish Peroxidase , Mandibular Nerve/anatomy & histology , Oxazines , Rats , Rats, Wistar , Suture Techniques , p-DimethylaminoazobenzeneABSTRACT
This study evaluates the use of sutureless laser welding for vasovasostomy. In 14 rodents, the left vas deferens underwent vasovasostomy using an albumin-based solder applied to the adventitia of the vas deferens. The solder contained the dye, indocyanine green, to allow selective absorption and denaturation by a fiber-coupled 800-nm diode laser. The right vas deferens served as a control, receiving conventional layered microsurgical repair. We used a removable 4/0 nylon stent and microclamps to appose the vas deferens during repair, with no need for stay sutures. The mean time to perform laser solder repair (23.5 min) and conventional repair (23.3 min) were not significantly different (P=0.91). However, examination after 8 weeks showed that granuloma formation (G) and patency (P) rates for the conventional suture technique (G, 14%; P, 93%) were significantly better than observed for the laser solder technique (G, 57%; P, 50%).
Subject(s)
Albumins/administration & dosage , Biocompatible Materials , Laser Coagulation/methods , Microsurgery/methods , Vas Deferens/surgery , Vasectomy/methods , Animals , Biodegradation, Environmental , Cattle , Coloring Agents/administration & dosage , Disease Models, Animal , Indocyanine Green/administration & dosage , Laser Coagulation/instrumentation , Male , Microsurgery/instrumentation , Rats , Rats, Wistar , Reference Values , Sensitivity and Specificity , Suture Techniques , Treatment OutcomeABSTRACT
We have generated 4.7 W of UV (255-nm) radiation with wall plug efficiency of 0.12% by frequency doubling the green (511-nm) output of a kinetically enhanced medium-scale copper-vapor laser (CVL) in cesium lithium borate (CLBO). Frequency doubling in beta -barium borate produced 3.9 W of UV radiation with wall plug efficiency of 0.1%. We found that conversion was better with CLBO because of the reduced constraints on CVL beam quality, less UV absorption, and smaller UV walk-off.
