Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 71
Filter
Add more filters










Publication year range
1.
J Cell Biol ; 222(4)2023 04 03.
Article in English | MEDLINE | ID: mdl-36912772

ABSTRACT

Septins are filamentous GTPases that play important but poorly characterized roles in ciliogenesis. Here, we show that SEPTIN9 regulates RhoA signaling at the base of cilia by binding and activating the RhoA guanine nucleotide exchange factor, ARHGEF18. GTP-RhoA is known to activate the membrane targeting exocyst complex, and suppression of SEPTIN9 causes disruption of ciliogenesis and mislocalization of an exocyst subunit, SEC8. Using basal body-targeted proteins, we show that upregulating RhoA signaling at the cilium can rescue ciliary defects and mislocalization of SEC8 caused by global SEPTIN9 depletion. Moreover, we demonstrate that the transition zone components, RPGRIP1L and TCTN2, fail to accumulate at the transition zone in cells lacking SEPTIN9 or depleted of the exocyst complex. Thus, SEPTIN9 regulates the recruitment of transition zone proteins on Golgi-derived vesicles by activating the exocyst via RhoA to allow the formation of primary cilia.


Subject(s)
Cilia , Septins , rhoA GTP-Binding Protein , Cilia/metabolism , Cytoplasm/metabolism , Guanine Nucleotide Exchange Factors/metabolism , Septins/genetics , Septins/metabolism , Signal Transduction , rhoA GTP-Binding Protein/metabolism
2.
Bioessays ; 44(3): e2100183, 2022 03.
Article in English | MEDLINE | ID: mdl-35001404

ABSTRACT

Hedgehog (Hh) signaling is a widely studied signaling pathway because of its critical roles during development and in cell homeostasis. Vertebrate canonical and non-canonical Hh signaling are typically assumed to be distinct and occur in different cellular compartments. While research has primarily focused on the canonical form of Hh signaling and its dependency on primary cilia - microtubule-based signaling hubs - an extensive list of crucial functions mediated by non-canonical Hh signaling has emerged. Moreover, amounting evidence indicates that canonical and non-canonical modes of Hh signaling are interlinked, and that they can overlap spatially, and in many cases interact functionally. Here, we discuss some of the many cellular effects of non-canonical signaling and discuss new evidence indicating inter-relationships with canonical signaling. We discuss how Smoothened (Smo), a key component of the Hh pathway, might coordinate such diverse downstream effects. Collectively, pursuit of questions such as those proposed here will aid in elucidating the full extent of Smo function in development and advance its use as a target for cancer therapeutics.


Subject(s)
Cilia , Hedgehog Proteins , Cilia/metabolism , Hedgehog Proteins/metabolism , Receptors, G-Protein-Coupled/metabolism , Signal Transduction
3.
J Biol Chem ; 296: 100680, 2021.
Article in English | MEDLINE | ID: mdl-33872598

ABSTRACT

Primary cilia are hubs for several signaling pathways, and disruption in cilia function and formation leads to a range of diseases collectively known as ciliopathies. Both ciliogenesis and cilia maintenance depend on vesicle trafficking along a network of microtubules and actin filaments toward the basal body. The DIAPH (Diaphanous-related) family of formins promote both actin polymerization and microtubule (MT) stability. Recently, we showed that the formin DIAPH1 is involved in ciliogenesis. However, the role of other DIAPH family members in ciliogenesis had not been investigated. Here we show that depletion of either DIAPH2 or DIAPH3 also disrupted ciliogenesis and cilia length. DIAPH3 depletion also reduced trafficking within cilia. To specifically examine the role of DIAPH3 at the base, we used fused full-length DIAPH3 to centrin, which targeted DIAPH3 to the basal body, causing increased trafficking to the ciliary base, an increase in cilia length, and formation of bulbs at the tips of cilia. Additionally, we confirmed that the microtubule-stabilizing properties of DIAPH3 are important for its cilia length functions and trafficking. These results indicate the importance of DIAPH proteins in regulating cilia maintenance. Moreover, defects in ciliogenesis caused by DIAPH depletion could only be rescued by expression of the specific family member depleted, indicating nonredundant roles for these proteins.


Subject(s)
Cilia/metabolism , Formins/metabolism , Actins/metabolism , Cell Line , Humans , Microtubules/metabolism
4.
Mol Biol Cell ; 32(3): 289-300, 2021 02 01.
Article in English | MEDLINE | ID: mdl-33263440

ABSTRACT

Septins are conserved GTP-binding cytoskeletal proteins that polymerize into filaments by end-to-end joining of hetero-oligomeric complexes. In human cells, both hexamers and octamers exist, and crystallography studies predicted the order of the hexamers to be SEPT7-SEPT6-SEPT2-SEPT2-SEPT6-SEPT7, while octamers are thought to have the same core, but with SEPT9 at the ends. However, based on this septin organization, octamers and hexamers would not be expected to copolymerize due to incompatible ends. Here we isolated hexamers and octamers of specific composition from human cells and show that hexamers and octamers polymerize individually and, surprisingly, with each other. Binding of the Borg homology domain 3 (BD3) domain of Borg3 results in distinctive clustering of each filament type. Moreover, we show that the organization of hexameric and octameric complexes is inverted compared with its original prediction. This revised septin organization is congruent with the organization and behavior of yeast septins suggesting that their properties are more conserved than was previously thought.


Subject(s)
Septins/metabolism , Septins/physiology , Animals , Cell Cycle Proteins/metabolism , Cytoskeleton/metabolism , HeLa Cells , Humans , Mammals/metabolism , Polymerization
5.
J Cell Biol ; 220(1)2021 01 04.
Article in English | MEDLINE | ID: mdl-33258871

ABSTRACT

Primary cilia function as critical signaling hubs whose absence leads to severe disorders collectively known as ciliopathies; our knowledge of ciliogenesis remains limited. We show that Smo induces ciliogenesis through two distinct yet essential noncanonical Hh pathways in several cell types, including neurons. Surprisingly, ligand activation of Smo induces autophagy via an LKB1-AMPK axis to remove the satellite pool of OFD1. This is required, but not sufficient, for ciliogenesis. Additionally, Smo activates the Gαi-LGN-NuMA-dynein axis, causing accumulation of a portion of OFD1 at centrioles in early ciliogenesis. Both pathways are critical for redistribution of BBS4 from satellites to centrioles, which is also mediated by OFD1 centriolar translocation. Notably, different Smo agonists, which activate Smo distinctly, activate one or the other of these pathways; only in combination they recapitulate the activity of Hh ligand. These studies provide new insight into physiological stimuli (Hh) that activate autophagy and promote ciliogenesis and introduce a novel role for the Gαi-LGN-NuMA-dynein complex in this process.


Subject(s)
Autophagy , Cilia/metabolism , Hedgehog Proteins/metabolism , Organogenesis , Signal Transduction , AMP-Activated Protein Kinase Kinases , Adenylate Kinase/metabolism , Autophagy/drug effects , Basal Bodies/drug effects , Basal Bodies/metabolism , Cell Cycle Proteins/metabolism , Cells, Cultured , Centrioles/drug effects , Centrioles/metabolism , Cilia/drug effects , Dyneins/metabolism , GTP-Binding Protein alpha Subunits, Gi-Go/metabolism , HeLa Cells , Humans , Microtubule-Associated Proteins/metabolism , Neurons/drug effects , Neurons/metabolism , Organogenesis/drug effects , Piperazines/pharmacology , Protein Serine-Threonine Kinases/metabolism , Protein Transport/drug effects , Proteins/metabolism , Proteolysis/drug effects , Pyridines/pharmacology , RNA, Small Interfering/metabolism , Retinal Pigment Epithelium/cytology , Serum/metabolism , Signal Transduction/drug effects , Smoothened Receptor/agonists , Smoothened Receptor/antagonists & inhibitors , Smoothened Receptor/metabolism
6.
FASEB J ; 34(12): 16516-16535, 2020 12.
Article in English | MEDLINE | ID: mdl-33124112

ABSTRACT

Primary cilia are critical hubs for several signaling pathways, and defects in ciliogenesis or cilia maintenance produce a range of diseases collectively known as ciliopathies. Ciliogenesis requires vesicle trafficking along a network of microtubules and actin filaments to the basal body. The DIAPH1 (Diaphanous-related formin) family of formins promotes both actin polymerization and EB1-dependent microtubule (MT) stability. EB1 and EB3 have previously been implicated in cilia biogenesis to carry out centrosome-related functions. However, the role of DIAPH1 proteins had not been examined. Here we show that the depletion of DIAPH1 decreased ciliogenesis, cilia length, and reduced trafficking within cilia. Additionally, both actin nucleating and microtubule-stabilizing properties of DIAPH1 are important for their cilia functions. To assess their roles in ciliogenesis in isolation, we targeted DIAPH1 specifically to the basal body, which caused an increase in cilia length and increased trafficking within cilia. Intriguingly, expression of DIAPH1 mutants associated with human deafness and microcephaly impaired ciliation and caused cilia elongation and bulb formation. These results suggest that the actin and microtubule functions of DIAPH1 proteins regulate cilia maintenance in part by regulating vesicular trafficking to the base of the primary cilia.


Subject(s)
Cell Movement/physiology , Cilia/metabolism , Cilia/physiology , Formins/metabolism , Protein Transport/physiology , Actin Cytoskeleton/metabolism , Actins/metabolism , Cells, Cultured , Centrosome/metabolism , Centrosome/physiology , Ciliopathies/metabolism , Humans , Microtubule-Associated Proteins/metabolism , Microtubules/metabolism , Microtubules/physiology
7.
Nat Cell Biol ; 21(10): 1234-1247, 2019 10.
Article in English | MEDLINE | ID: mdl-31570833

ABSTRACT

Phosphoinositides have a pivotal role in the maturation of nascent phagosomes into microbicidal phagolysosomes. Following degradation of their contents, mature phagolysosomes undergo resolution, a process that remains largely uninvestigated. Here we studied the role of phosphoinositides in phagolysosome resolution. Phosphatidylinositol-4-phosphate (PtdIns(4)P), which is abundant in maturing phagolysosomes, was depleted as they tubulated and resorbed. Depletion was caused, in part, by transfer of phagolysosomal PtdIns(4)P to the endoplasmic reticulum, a process mediated by oxysterol-binding protein-related protein 1L (ORP1L), a RAB7 effector. ORP1L formed discrete tethers between the phagolysosome and the endoplasmic reticulum, resulting in distinct regions with alternating PtdIns(4)P depletion and enrichment. Tubules emerged from PtdIns(4)P-rich regions, where ADP-ribosylation factor-like protein 8B (ARL8B) and SifA- and kinesin-interacting protein/pleckstrin homology domain-containing family M member 2 (SKIP/PLEKHM2) accumulated. SKIP binds preferentially to monophosphorylated phosphoinositides, of which PtdIns(4)P is most abundant in phagolysosomes, contributing to their tubulation. Accordingly, premature hydrolysis of PtdIns(4)P impaired SKIP recruitment and phagosome resolution. Thus, resolution involves phosphoinositides and tethering of phagolysosomes to the endoplasmic reticulum.


Subject(s)
Endoplasmic Reticulum/metabolism , Monocytes/metabolism , Phagosomes/metabolism , Phosphatidylinositol Phosphates/metabolism , Receptors, Steroid/genetics , Signal Transduction , ADP-Ribosylation Factors/genetics , ADP-Ribosylation Factors/metabolism , Animals , CRISPR-Cas Systems , Endoplasmic Reticulum/ultrastructure , Gene Editing , Gene Expression Regulation , Humans , Mice , Monocytes/ultrastructure , Phagocytosis , Phagosomes/ultrastructure , Primary Cell Culture , Proteolysis , RAW 264.7 Cells , RNA, Small Interfering/genetics , RNA, Small Interfering/metabolism , Receptors, Steroid/antagonists & inhibitors , Receptors, Steroid/metabolism , Vesicular Transport Proteins/genetics , Vesicular Transport Proteins/metabolism , rab GTP-Binding Proteins/genetics , rab GTP-Binding Proteins/metabolism , rab7 GTP-Binding Proteins
8.
Dev Cell ; 50(3): 283-295.e5, 2019 08 05.
Article in English | MEDLINE | ID: mdl-31231038

ABSTRACT

Scavenger receptor B1 (SR-B1), the main receptor for high-density lipoprotein (HDL), is key in preventing atherosclerosis. It removes cholesterol from HDL, returning the lipid-poor lipoprotein to the circulation. To study the mechanisms controlling SR-B1 dynamics at the plasma membrane and its internalization rate, we developed a single-chain variable fragment (ScFv) antibody to image the receptor in live cells and track the behavior of single SR-B1 molecules. Unlike transferrin receptors, cholera-toxin-binding gangliosides, and bulk membrane markers, SR-B1 was internalized only marginally over hours. Plasmalemmal retention was not attributable to its C-terminal PDZ-binding domain or to attachment to the cortical cytoskeleton. Instead, SR-B1 undergoes multimerization into large metastable clusters that, despite being mobile in the membrane, fail to enter endocytic pathways. SR-B1 multimerization was impaired by mutating its C-terminal leucine zipper and by disrupting actin polymerization, causing rapid receptor internalization. Multimerization and plasmalemmal retention are critical for SR-B1 function.


Subject(s)
Cell Membrane/metabolism , Protein Multimerization , Scavenger Receptors, Class B/metabolism , Actin Cytoskeleton/metabolism , Animals , CHO Cells , Cricetinae , Cricetulus , Endocytosis , Hep G2 Cells , Humans , Leucine Zippers , Mutation , Scavenger Receptors, Class B/chemistry , Scavenger Receptors, Class B/genetics
9.
Cytoskeleton (Hoboken) ; 76(1): 63-72, 2019 01.
Article in English | MEDLINE | ID: mdl-30176126

ABSTRACT

Septins are a conserved family of GTPases that associate with numerous components of the cytoskeleton and the inner leaflet of the plasma membrane. These proteins are involved in many biological processes, including cell division and membrane trafficking, and serving as a scaffolding component of the cytoskeleton used to recruit other proteins and form diffusion barriers to maintain the composition of membrane domains. In order to carry out their cellular functions, septins undergo interactions via their NC or G interfaces to form heteromeric rod-like structures that can polymerize into filaments and associate laterally into bundles. While electron microscopy studies of affinity-tagged and purified Saccharomyces cerevisiae septin complexes have provided evidence for this periodic organization and in-registry lateral bundling in vitro, the in-vivo arrangement of stress fiber-associated septin bundles in mammalian cells remains poorly characterized. We report here on a direct stochastic optical reconstruction microscopy and photoactivated localization microscopy study of the 2D spatial distribution of septins in mammalian cells. From simulated and experimental results, we show the effects of labeling method, labeling efficiency, and fluorescent emitter photophysics on image reconstruction and interpretation. Our experimental results are consistent with septin organization by polymerization of hetero-octamers and an approximate 30-35 nm periodicity between subsequent units of SEPT2-SEPT2 or SEPT9-SEPT9.


Subject(s)
Septins/metabolism , Animals , Mammals , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/metabolism
10.
Cell Microbiol ; 20(10): e12866, 2018 10.
Article in English | MEDLINE | ID: mdl-29885024

ABSTRACT

Actin nucleators and their binding partners play crucial roles during Salmonella invasion, but how these factors are dynamically coordinated remains unclear. Here, we show that septins, a conserved family of GTP binding proteins, play a role during the early stages of Salmonella invasion. We demonstrate that septins are rapidly enriched at sites of bacterial entry and contribute to the morphology of invasion ruffles. We found that SEPTIN2, SEPTIN7, and SEPTIN9 are required for efficient bacterial invasion. Septins contributed to the recruitment of ROCK2 kinase during Salmonella invasion, and the downstream activation of the actin nucleating protein FHOD1. In contrast, activation of the ROCK2 substrate myosin II, which is known to be required for Salmonella enterica serovar Typhimurium invasion, did not require septins. Collectively, our studies provide new insight into the mechanisms involved in Salmonella invasion of host cells.


Subject(s)
Actins/metabolism , Myosins/metabolism , Salmonella Infections/pathology , Salmonella typhimurium/pathogenicity , Septins/metabolism , Cell Line, Tumor , Fetal Proteins/metabolism , Formins , HeLa Cells , Humans , Nuclear Proteins/metabolism , RNA Interference , RNA, Small Interfering/genetics , Salmonella typhimurium/genetics , rho-Associated Kinases/metabolism
11.
BMC Biol ; 15(1): 102, 2017 10 31.
Article in English | MEDLINE | ID: mdl-29089042

ABSTRACT

Discoveries spanning several decades have pointed to vital membrane lipid trafficking pathways involving both vesicular and non-vesicular carriers. But the relative contributions for distinct membrane delivery pathways in cell growth and organelle biogenesis continue to be a puzzle. This is because lipids flow from many sources and across many paths via transport vesicles, non-vesicular transfer proteins, and dynamic interactions between organelles at membrane contact sites. This forum presents our latest understanding, appreciation, and queries regarding the lipid transport mechanisms necessary to drive membrane expansion during organelle biogenesis and cell growth.


Subject(s)
Cell Cycle , Lipid Metabolism , Organelle Biogenesis , Biological Transport , Cell Membrane/metabolism
12.
Front Cell Dev Biol ; 5: 36, 2017.
Article in English | MEDLINE | ID: mdl-28428954

ABSTRACT

Septins are a family of GTP-binding proteins that associate with cellular membranes and the cytoskeleton. Their ability to polymerize into filamentous structures permits them to serve as diffusion barriers for membrane proteins and as multi-molecular scaffolds that recruit components of signaling pathways. At the cellular level, septins contribute to the regulation of numerous processes, including cytokinesis, cell polarity, cell migration, and many others. In this review, we discuss emerging evidence for roles of mammalian septins in the biogenesis and function of flagella and cilia, and how this may impact human diseases such as ciliopathies.

13.
J Cell Biol ; 216(2): 367-377, 2017 02.
Article in English | MEDLINE | ID: mdl-28108526

ABSTRACT

Lipid exchange between the endoplasmic reticulum (ER) and peroxisomes is necessary for the synthesis and catabolism of lipids, the trafficking of cholesterol, and peroxisome biogenesis in mammalian cells. However, how lipids are exchanged between these two organelles is not understood. In this study, we report that the ER-resident VAMP-associated proteins A and B (VAPA and VAPB) interact with the peroxisomal membrane protein acyl-CoA binding domain containing 5 (ACBD5) and that this interaction is required to tether the two organelles together, thereby facilitating the lipid exchange between them. Depletion of either ACBD5 or VAP expression results in increased peroxisome mobility, suggesting that VAP-ACBD5 complex acts as the primary ER-peroxisome tether. We also demonstrate that tethering of peroxisomes to the ER is necessary for peroxisome growth, the synthesis of plasmalogen phospholipids, and the maintenance of cellular cholesterol levels. Collectively, our data highlight the importance of VAP-ACBD5-mediated contact between the ER and peroxisomes for organelle maintenance and lipid homeostasis.


Subject(s)
Adaptor Proteins, Signal Transducing/metabolism , Cholesterol/metabolism , Endoplasmic Reticulum/metabolism , Intracellular Membranes/metabolism , Membrane Proteins/metabolism , Peroxisomes/metabolism , Phospholipids/metabolism , Vesicular Transport Proteins/metabolism , Adaptor Proteins, Signal Transducing/genetics , Animals , COS Cells , Chlorocebus aethiops , HEK293 Cells , HeLa Cells , Homeostasis , Humans , Membrane Proteins/genetics , Microscopy, Confocal , Protein Interaction Domains and Motifs , RNA Interference , Signal Transduction , Time Factors , Transfection , Vesicular Transport Proteins/genetics
14.
Mol Cell Proteomics ; 14(7): 1927-45, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25944883

ABSTRACT

Enteropathogenic Escherichia coli (EPEC) uses a type III secretion system (T3SS) to directly translocate effector proteins into host cells where they play a pivotal role in subverting host cell signaling needed for disease. However, our knowledge of how EPEC affects host protein phosphorylation is limited to a few individual protein studies. We employed a quantitative proteomics approach to globally map alterations in the host phosphoproteome during EPEC infection. By characterizing host phosphorylation events at various time points throughout infection, we examined how EPEC dynamically impacts the host phosphoproteome over time. This experimental setup also enabled identification of T3SS-dependent and -independent changes in host phosphorylation. Specifically, T3SS-regulated events affected various cellular processes that are known EPEC targets, including cytoskeletal organization, immune signaling, and intracellular trafficking. However, the involvement of phosphorylation in these events has thus far been poorly studied. We confirmed the MAPK family as an established key host player, showed its central role in signal transduction during EPEC infection, and extended the repertoire of known signaling hubs with previously unrecognized proteins, including TPD52, CIN85, EPHA2, and HSP27. We identified altered phosphorylation of known EPEC targets, such as cofilin, where the involvement of phosphorylation has so far been undefined, thus providing novel mechanistic insights into the roles of these proteins in EPEC infection. An overlap of regulated proteins, especially those that are cytoskeleton-associated, was observed when compared with the phosphoproteome of Shigella-infected cells. We determined the biological relevance of the phosphorylation of a novel protein in EPEC pathogenesis, septin-9 (SEPT9). Both siRNA knockdown and a phosphorylation-impaired SEPT9 mutant decreased bacterial adherence and EPEC-mediated cell death. In contrast, a phosphorylation-mimicking SEPT9 mutant rescued these effects. Collectively, this study provides the first global analysis of phosphorylation-mediated processes during infection with an extracellular, diarrheagenic bacterial pathogen.


Subject(s)
Enteropathogenic Escherichia coli/pathogenicity , Host-Pathogen Interactions , Phosphoproteins/metabolism , Proteome/metabolism , Proteomics/methods , Signal Transduction , Amino Acid Sequence , Bacterial Secretion Systems , Epithelial Cells/metabolism , Epithelial Cells/microbiology , Escherichia coli Infections/metabolism , Escherichia coli Infections/microbiology , HeLa Cells , Humans , Molecular Sequence Data , Phosphoproteins/chemistry , Phosphorylation , Septins/metabolism , Shigella/metabolism , Virulence
15.
J Cell Biol ; 208(3): 259-71, 2015 Feb 02.
Article in English | MEDLINE | ID: mdl-25646084

ABSTRACT

Biological membranes segregate into specialized functional domains of distinct composition, which can persist for the entire life of the cell. How separation of their lipid and (glyco)protein components is generated and maintained is not well understood, but the existence of diffusional barriers has been proposed. Remarkably, the physical nature of such barriers and the manner whereby they impede the free diffusion of molecules in the plane of the membrane has rarely been studied in depth. Moreover, alternative mechanisms capable of generating membrane inhomogeneity are often disregarded. Here we describe prototypical biological systems where membrane segregation has been amply documented and discuss the role of diffusional barriers and other processes in the generation and maintenance of their structural and functional compartmentalization.


Subject(s)
Cell Membrane/metabolism , Membrane Lipids/metabolism , Membrane Proteins/metabolism , Animals , Cell Membrane/ultrastructure , Cell Membrane Structures/metabolism , Cytokinesis , Diffusion , Humans , Protein Transport
16.
Science ; 344(6180): 208-11, 2014 Apr 11.
Article in English | MEDLINE | ID: mdl-24723613

ABSTRACT

Genome-wide characterization of the in vivo cellular response to perturbation is fundamental to understanding how cells survive stress. Identifying the proteins and pathways perturbed by small molecules affects biology and medicine by revealing the mechanisms of drug action. We used a yeast chemogenomics platform that quantifies the requirement for each gene for resistance to a compound in vivo to profile 3250 small molecules in a systematic and unbiased manner. We identified 317 compounds that specifically perturb the function of 121 genes and characterized the mechanism of specific compounds. Global analysis revealed that the cellular response to small molecules is limited and described by a network of 45 major chemogenomic signatures. Our results provide a resource for the discovery of functional interactions among genes, chemicals, and biological processes.


Subject(s)
Cells/drug effects , Drug Evaluation, Preclinical/methods , Drug Resistance/genetics , Gene Regulatory Networks , Genome-Wide Association Study/methods , Small Molecule Libraries/pharmacology , Cell Line, Tumor , Haploinsufficiency , Humans , Pharmacogenetics , Saccharomyces cerevisiae/drug effects , Saccharomyces cerevisiae/genetics
17.
Curr Biol ; 24(8): R324-7, 2014 Apr 14.
Article in English | MEDLINE | ID: mdl-24735857

ABSTRACT

Septins are a family of membrane-associated filamentous proteins that are essential in many biological processes, such as cell division. A recent study has provided the first visualization of septin filament formation in real time, leading to important new insights into their organization.


Subject(s)
Septins/chemistry
18.
Int Rev Cell Mol Biol ; 310: 289-339, 2014.
Article in English | MEDLINE | ID: mdl-24725429

ABSTRACT

Septins are a family of GTP-binding proteins that assemble into cytoskeletal filaments. Unlike other cytoskeletal components, septins form ordered arrays of defined stoichiometry that can polymerize into long filaments and bundle laterally. Septins associate directly with membranes and have been implicated in providing membrane stability and serving as diffusion barriers for membrane proteins. In addition, septins bind other proteins and have been shown to function as multimolecular scaffolds by recruiting components of signaling pathways. Remarkably, septins participate in a spectrum of cellular processes including cytokinesis, ciliogenesis, cell migration, polarity, and cell-pathogen interactions. Given their breadth of functions, it is not surprising that septin abnormalities have also been linked to human diseases. In this review, we discuss the current knowledge of septin structure, assembly and function, and discuss these in the context of human disease.


Subject(s)
GTP-Binding Proteins/metabolism , Gene Expression Regulation , Septins/metabolism , Animals , Apoptosis , Cell Cycle , Cell Division , Cell Membrane/metabolism , Cell Movement , Cytokines/metabolism , Cytoskeleton/metabolism , GTP Phosphohydrolases/metabolism , Humans , Mice , Microtubules/metabolism , Neoplasms/metabolism , Protein Structure, Tertiary , Saccharomycetales/metabolism
19.
PLoS One ; 9(3): e91471, 2014.
Article in English | MEDLINE | ID: mdl-24626111

ABSTRACT

Each membrane fusion event along the secretory and endocytic pathways requires a specific set of SNAREs to assemble into a 4-helical coiled-coil, the so-called trans-SNARE complex. Although most SNAREs contribute one helix to the trans-SNARE complex, members of the SNAP-25 family contribute two helixes. We report the characterization of the Drosophila homologue of SNAP-29 (dSNAP-29), which is expressed throughout development. Unlike the other SNAP-25 like proteins in fruit fly (i.e., dSNAP-25 and dSNAP-24), which form SDS-resistant SNARE complexes with their cognate SNAREs, dSNAP-29 does not participate in any SDS-resistant complexes, despite its interaction with dsyntaxin1 and dsyntaxin16 in vitro. Immunofluorescence studies indicated that dSNAP-29 is distributed in various tissues, locating in small intracellular puncta and on the plasma membrane, where it associates with EH domain-containing proteins implicated in the endocytic pathway. Overexpression and RNAi studies suggested that dSNAP-29 mediates an essential process in Drosophila development.


Subject(s)
Cell Membrane/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , SNARE Proteins/metabolism , Animals , Crosses, Genetic , Microscopy, Fluorescence , Phenotype , Protein Binding , Protein Structure, Tertiary , RNA Interference , Syntaxin 1/metabolism , Syntaxin 16/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...