ABSTRACT
This study evaluated the semen quality of Santa Ines sheep fed diets containing different levels of whole cottonseed (WCS). Twenty sheep were distributed into four groups that were given diets with 0; 20; 30; 40% of WCS. For this study the semen was collected by electroejaculation, and a monthly andrological examination was performed during nine months. The analyzed variables were: scrotal circumference (SC), appearance and color of the ejaculate, progressive motility (PM), vigor, mass movement (MM), total sperm concentration (spzX109), sperm morphology (SM) and blood hematocrit. The SC had no difference among diets, with mean 31.83 cm. The animals fed diets with WCS, presented mean intake of 9.16 mg gossypol kg BW, and were prone to present greenish semen, and appearance ranging from thin creamy to watery. The PM decreased linearly (p 0.05) with increasing level of WCS, while the vigor had a quadratic trend. The total defects increased linearly in relation to reference values described in literature. The WSC had no influence on hematocrit levels, but did affect the PM and vigor of spermatozoa, thus not recommended to diets of animals for breeding.
ABSTRACT
This study evaluated the semen quality of Santa Ines sheep fed diets containing different levels of whole cottonseed (WCS). Twenty sheep were distributed into four groups that were given diets with 0; 20; 30; 40% of WCS. For this study the semen was collected by electroejaculation, and a monthly andrological examination was performed during nine months. The analyzed variables were: scrotal circumference (SC), appearance and color of the ejaculate, progressive motility (PM), vigor, mass movement (MM), total sperm concentration (spzX109), sperm morphology (SM) and blood hematocrit. The SC had no difference among diets, with mean 31.83 cm. The animals fed diets with WCS, presented mean intake of 9.16 mg gossypol kg BW, and were prone to present greenish semen, and appearance ranging from thin creamy to watery. The PM decreased linearly (p 0.05) with increasing level of WCS, while the vigor had a quadratic trend. The total defects increased linearly in relation to reference values described in literature. The WSC had no influence on hematocrit levels, but did affect the PM and vigor of spermatozoa, thus not recommended to diets of animals for breeding.
ABSTRACT
Aiming to evaluate in vitro effect of different concentrations of glutathione reduced (GSH) in skimmed-milk and glycerol 7% it was used semen from five Boer bucks. After collect and evaluation, a pool of samples was diluted in skimmed-milk and glycerol 7% plus antioxidant: G1) Control; G2) GSH 2mM mL-1; G3) GSH 5mM mL-1 and G4) GSH 7mM mL-1. Samples were frozen in straws (0.25mL) and stored at -196°C. After thawing, samples were subjected to integrity of the plasma membrane (iMP) and acrosomal (iAc), mitochondrial membrane potential (MMP), kinematic and ultrastructure analysis. Control and GSH (2, 5 and 7mM mL-1) groups did no differ (P>0.05) in iMP, iAc, PMM and kinematic parameters. In the ultrastructural analysis, percentages of acrosome and plasma membrane (tail and head region) intact did not differ (P>0.05) between groups. However, Control group had higher percentage (P 0.05) of gametes with intact axonemes than those of GSH (2, 5 and 7mM mL-1) groups. Higher percentage (P 0.05) of sperms with intact mitochondrias were observed on Control group than those of GSH (5 and 7mM mL-1). It can be concluded that the GSH (2, 5 and 7mM mL-1) addition in skimmed-milk diluent to freeze goat semen did not preserve sperm integrity.
Visando avaliar o efeito da adição de glutationa reduzida (GSH) ao diluente de congelação de sêmen caprino à base de leite desnatado, utilizou-se sêmen de cinco reprodutores Boer. Após colheita e avaliação, procedeu-se à formação do pool dos ejaculados e diluição em leite desnatado e glicerol 7%, acrescido de antioxidantes: G1) Controle; G2) GSH 2mM mL-1; G3) GSH 5mM mL-1 e G4) GSH 7mM mL-1. As amostras foram congeladas em palhetas (0,25mL) e armazenadas a -196°C. Após descongelação, avaliou-se a integridade de membrana plasmática (iMP) e acrossomal (iAc), potencial de membrana mitocondrial (PMM), cinética e ultraestrutura. Os grupos Controle e GSH (2, 5 e 7mM mL-1) não diferiram (P>0,05) em iMP, iAc, PMM e cinética. Na análise ultraestrutural, os porcentuais de membrana plasmática (cabeça e cauda) e acrossoma íntegros não diferiram (P>0,05) entre grupos. Todavia, o grupo Controle apresentou maior porcentual (P 0,05) de gametas com axonema íntegros do que os de GSH (2, 5 e 7mM mL-1). Maior porcentagem (P 0,05) de espermatozoides com mitocôndrias íntegras foi observada no grupo Controle do que nos de GSH (5 e 7 mM mL-1). Conclui-se que a adição de GSH (2, 5 e 7mM mL-1) em diluente de congelação de sêmen caprino, à base de leite desnatado, não preserva a integridade dos espermatozoides.
ABSTRACT
Aiming to evaluate in vitro effect of different concentrations of glutathione reduced (GSH) in skimmed-milk and glycerol 7% it was used semen from five Boer bucks. After collect and evaluation, a pool of samples was diluted in skimmed-milk and glycerol 7% plus antioxidant: G1) Control; G2) GSH 2mM mL-1; G3) GSH 5mM mL-1 and G4) GSH 7mM mL-1. Samples were frozen in straws (0.25mL) and stored at -196°C. After thawing, samples were subjected to integrity of the plasma membrane (iMP) and acrosomal (iAc), mitochondrial membrane potential (MMP), kinematic and ultrastructure analysis. Control and GSH (2, 5 and 7mM mL-1) groups did no differ (P>0.05) in iMP, iAc, PMM and kinematic parameters. In the ultrastructural analysis, percentages of acrosome and plasma membrane (tail and head region) intact did not differ (P>0.05) between groups. However, Control group had higher percentage (P 0.05) of gametes with intact axonemes than those of GSH (2, 5 and 7mM mL-1) groups. Higher percentage (P 0.05) of sperms with intact mitochondrias were observed on Control group than those of GSH (5 and 7mM mL-1). It can be concluded that the GSH (2, 5 and 7mM mL-1) addition in skimmed-milk diluent to freeze goat semen did not preserve sperm integrity.
Visando avaliar o efeito da adição de glutationa reduzida (GSH) ao diluente de congelação de sêmen caprino à base de leite desnatado, utilizou-se sêmen de cinco reprodutores Boer. Após colheita e avaliação, procedeu-se à formação do pool dos ejaculados e diluição em leite desnatado e glicerol 7%, acrescido de antioxidantes: G1) Controle; G2) GSH 2mM mL-1; G3) GSH 5mM mL-1 e G4) GSH 7mM mL-1. As amostras foram congeladas em palhetas (0,25mL) e armazenadas a -196°C. Após descongelação, avaliou-se a integridade de membrana plasmática (iMP) e acrossomal (iAc), potencial de membrana mitocondrial (PMM), cinética e ultraestrutura. Os grupos Controle e GSH (2, 5 e 7mM mL-1) não diferiram (P>0,05) em iMP, iAc, PMM e cinética. Na análise ultraestrutural, os porcentuais de membrana plasmática (cabeça e cauda) e acrossoma íntegros não diferiram (P>0,05) entre grupos. Todavia, o grupo Controle apresentou maior porcentual (P 0,05) de gametas com axonema íntegros do que os de GSH (2, 5 e 7mM mL-1). Maior porcentagem (P 0,05) de espermatozoides com mitocôndrias íntegras foi observada no grupo Controle do que nos de GSH (5 e 7 mM mL-1). Conclui-se que a adição de GSH (2, 5 e 7mM mL-1) em diluente de congelação de sêmen caprino, à base de leite desnatado, não preserva a integridade dos espermatozoides.
ABSTRACT
The aim of the work was to evaluate the rates of estrous, superovulatory response and embryos recovery in Moxotó goats after three treatments with porcine FSH. Twenty goats were synchronised with medroxyprogesterone acetate sponges (60 mg for 11days) and cloprostenol (l00 mug on ninth day). The animals were superovulated with 250 IU of FSH-p in 8 decreasing doses at intervals of 12 hours. The repetition of superovulatory treatment decreased estrous symptoms, but didnt affect the ovulation rate and decreased the regressed corpus luteum rate. The embryos recovery was affected by regressed corpus luteum and embryo collection technique.
O objetivo deste trabalho foi avaliar as taxas de manifestação de sintomas de estro, de resposta superovulatória e de recuperação de embriões em 20 cabras da raça Moxotó em três tratamentos consecutivos, em intervalos de 56 dias. O ciclo estral das fêmeas foi sincronizado com esponjas vaginais contendo 60 mg de acetato de medroxiprogesterona (MGA) durante 11 dias e com 100 mig de cloprostenol (PGF2alfa ) administrado no nono dia. Neste dia, iniciou-se o tratamento superovulatório com 250 UI de FSH-p por cabra, fracionadas em oito doses decrescentes, com intervalo de 12 horas. A repetição do tratamento superovulatório com FSH-p diminuiu a taxa de manifestação de sintomas de estro, porém não afetou a taxa de ovulação e reduziu a taxa de regressão prematura de corpos lúteos. A taxa de recuperação de embriões foi influenciada pela ocorrência de regressão prematura de corpos lúteos e pela ação das repetidas colheitas de embriões sobre o genital. Não foi possível avaliar o efeito das repetidas superovulações sobre a taxa de recuperação de embriões.