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J Food Sci ; 74(2): C100-5, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19323722

ABSTRACT

Stewed beef and grilled dry aged beef were analyzed as part of an in-depth analytical program, with the aim of creating new flavors incorporating only compounds identified in the target foods and identifying new synthesis targets. In-house GC-MS analyses of several types of cooked beef have identified over 1000 volatile and semivolatile components; many for the 1st time. Among the semivolatiles detected were ten 2, 5-diketopiperazines (cyclic dipeptides) previously unreported in beef. These cyclic dipeptides are cis-cyclo(L-Ile-L-Pro), cis-cyclo(L-Leu-L-Pro), cis-cyclo(L-Pro-L-Pro), cis-cyclo(L-Pro-L-Val), cis-cyclo(L-Ala-L-Pro), cyclo(Gly-L-Pro), cyclo(Gly-L-Leu), cis-cyclo(L-Met-L-Pro), cis-cyclo(L-Phe-L-Pro), and cis-cyclo(L-Phe-L-Val). All 10 cyclic dipeptides were synthesized and evaluated organoleptically. Among them cis-cyclo(L-Leu-L-Pro), cis-cyclo(L-Met-L-Pro), and cis-cyclo(L-Phe-L-Pro) were found to be of particular organoleptic interest.


Subject(s)
Diketopiperazines/analysis , Meat/analysis , Animals , Cattle , Chromatography, Gas , Cooking , Diketopiperazines/chemistry , Diketopiperazines/isolation & purification , Dipeptides/analysis , Dipeptides/isolation & purification , Gas Chromatography-Mass Spectrometry , Humans , Odorants , Taste , Volatile Organic Compounds/analysis , Volatile Organic Compounds/isolation & purification
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