ABSTRACT
Detection rate of G. intestionalis in feces with direct microscopy has been compared with the immuno-enzymatic technique detecting protein GSA 65 with Alexon Inc., ProSpec T/Giardia reagents kit. The results obtained with both methods have further been compared with those obtained by microscopic examination of the duodenal content. Detectability of Giardia intestinalis with EIA technique with the use of ready-made kit has been assessed. Feces have been collected from 371 patients. Protein GSA 65 has been present in 170 samples, 45.8%, examined with the use of ProSpec-T/Giardia kit. Giardia intestinalis cysts have been detected microscopically in the feces of 37 patients, i.e., in 22.3%. Microscopic examinations carried out by three independent examinators have shown marked diversity in the rate of positive results, being 0.1% (examinator A), 28.6% (B), and 45.2% (C). Comparison of G. intestinale detectability of all 3 techniques used have shown absence of protein GSA 65 in 2 out of 9 examined patients. However, trophozoites have been present in the duodenal content. Test performed with kits made by Alexon Inc. and DIALAB have given 45.8% and 60.7% of the positive results, respectively.
Subject(s)
Feces/parasitology , Giardia lamblia/isolation & purification , Giardiasis/parasitology , Adolescent , Adult , Animals , Child , Child, Preschool , Humans , Immunoenzyme Techniques , Infant , Microscopy/methods , Middle Aged , Reproducibility of ResultsABSTRACT
The clinical picture of Lyme borreliosis in children in an early stage of the disease was presented. Treatment with Amoxicillin or Doxycycline led to the regression of clinical symptoms and elimination of specific antibodies within a three-month period of therapy in all cases.
Subject(s)
Lyme Disease/diagnosis , Adolescent , Amoxicillin/therapeutic use , Antibodies, Bacterial/analysis , Borrelia burgdorferi Group/immunology , Child , Child, Preschool , Doxycycline/therapeutic use , Enzyme-Linked Immunosorbent Assay , Erythema Chronicum Migrans/etiology , Female , Humans , Lyme Disease/drug therapy , MaleABSTRACT
In the group of 81 children infected with Giardia lamblia, the chosen parameters of cellular and humoral immunity as well as the effect of allergic costitution on the clinical picture of giardiasis were evaluated. Both cellular and humoral immunity disorders were found in patients with a chronic form of infestation. An allergic constitution coexisting with giardiasis promotes the chronicity of this parasitosis and a progressing allergization.
Subject(s)
Giardiasis/immunology , Adolescent , Antibody Formation , Child , Child, Preschool , Chronic Disease , Humans , Immunity, Cellular , Immunoglobulins/analysisABSTRACT
In 34 rabbits infected with Trichinella spiralis larvae, lysozyme activity of the serum was determined in the period of one year. We have observed an increased enzyme activity both in acute and chronic period of the disease. Rabbits infected with heavy doses of Trichinella spiralis larvae showed only a minute increase of serum lysozyme activity.
Subject(s)
Muramidase/blood , Trichinellosis/enzymology , Animals , Rabbits , Time Factors , Trichinellosis/bloodABSTRACT
The number of leucocytes, neutrophils and eosinophils was determined during one year observation in 34 rabbits after an infection with Trichinella spiralis in the doses of 15,000, 20,000 and 30,000 larvae. Granulocyte reactions were already observed on the third day after the infection, they lasted at least for 3 months and depended on the intensity of the infection.
