ABSTRACT
The optical density indices of nucleoli and cytoplasm of male generative cells during microsporogenesis have been estimated for wheat, rye and F1 of wheat-rye hybrids using RNA staining. The correlation between RNA con tent in the nucleolus and the cytoplasm of investigated cells has been estimated. The dynamics of correlation between the nucleolus volume and RNA content in the nucleolus/ the cytoplasm has been shown for wheat and hybrids cells during microsporogenesis. The essential differences depending on genotype for quantitative karyometrical and cytochemical character expression have been determined for parental forms, as well as dependence of expression of these characters in the cells of FI wheat-rye hybrids on wheat maternal form.
Subject(s)
Cell Nucleolus/ultrastructure , Chimera/genetics , Cytoplasm/ultrastructure , RNA, Plant/genetics , Secale/ultrastructure , Triticum/ultrastructure , Cell Nucleolus/genetics , Chimera/physiology , Cytoplasm/genetics , Secale/genetics , Secale/physiology , Spores/genetics , Triticum/genetics , Triticum/physiologyABSTRACT
The quantity of nucleolei during microsporogenesis of wheat, rye and F1 of wheat-rye hybrids has been investigated. Dependence of nucleoli quantity from microsporogenesis stage and number of nucleolar organizer regions in chromosomes have been shown. The volumes of nuclei and nucleoli as well as nucleus-nucleolus ratio have been calculated. The changes of these indices during microsporogenesis were regular. The possibility of using cariometrical indices as cytological markers of rRNA gene expression in the process of microsporogenesis has been substantiated.
