Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Respir Res ; 14: 114, 2013 Oct 29.
Article in English | MEDLINE | ID: mdl-24168756

ABSTRACT

BACKGROUND: Myeloid Dendritic cells are key drivers of inflammation in smoke-related lung diseases, whereas plasmacytoid DCs play a crucial role in the defense against infections. Effects of inhaled corticosteroids (ICS) on airway DCs in smokers are unknown. METHODS: In this randomized, double-blind, placebo-controlled clinical trial, 45 active cigarette smokers inhaled placebo, fluticasone or fluticasone plus salmeterol twice daily for 4 weeks. Bronchoalveolar lavage fluid DCs were analyzed using four-color flow cytometry before and after the inhalation period. In addition, fluticasone effects were tested on T-cell proliferation in co-cultures with blood myeloid DCs from smokers. RESULTS: Inhalation of fluticasone plus salmeterol, but not fluticasone alone or placebo, reduced endobronchial concentrations of myeloid DCs (median decrease: 24%), macrophages (median decrease: 26%) and neutrophils (median decrease: 76%). In contrast, fluticasone reduced plasmacytoid DC concentrations independently of salmeterol. There were no changes in the expression of function-associated surface molecules on myeloid DC (such as CD1a, Langerin, BDCA-1, CD83 or CCR5) in all groups after treatment. Fluticasone (either alone or in combination with salmeterol) suppressed T-cell proliferation in co-cultures with blood myeloid DCs from smokers. CONCLUSIONS: Resistance to ICS monotherapy in smokers might in part be due to lacking effects on airway myeloid DCs, whereas the increased risk for infections during ICS therapy could be attributable to a reduction in plasmacytoid DCs. Combination therapy of fluticasone with salmeterol is associated with a reduction in airway myeloid DCs, but also airway macrophages and neutrophils. TRIAL REGISTRATION: Registered at ClinicalTrials.gov (identifier: NCT00908362) and the European Clinical Trial Database, EudraCT (identifier: 2009-009459-40).


Subject(s)
Androstadienes/pharmacology , Bronchi/drug effects , Bronchi/pathology , Bronchodilator Agents/pharmacology , Dendritic Cells/drug effects , Dendritic Cells/pathology , Smoking/pathology , Administration, Inhalation , Adult , Albuterol/administration & dosage , Albuterol/analogs & derivatives , Albuterol/pharmacology , Androstadienes/administration & dosage , Bronchoalveolar Lavage Fluid , Bronchodilator Agents/administration & dosage , Cell Proliferation/drug effects , Coculture Techniques , Double-Blind Method , Flow Cytometry , Fluticasone , Humans , Macrophages, Alveolar/drug effects , Macrophages, Alveolar/pathology , Male , Middle Aged , Neutrophils/pathology , Salmeterol Xinafoate , T-Lymphocytes/drug effects , T-Lymphocytes/pathology
SELECTION OF CITATIONS
SEARCH DETAIL
...