Subject(s)
Antigen-Antibody Complex/isolation & purification , Immunosorbent Techniques , Immunosorbents/isolation & purification , Magnetics , Fluorescent Antibody Technique/instrumentation , Immunosorbent Techniques/instrumentation , Ligands , Magnetics/instrumentation , Microbiological Techniques/instrumentation , Polymers , PowdersSubject(s)
Immunosorbent Techniques , Acrylic Resins , Gangliosides , Humans , Immunoglobulins , SepharoseABSTRACT
A variant of EIA techniques for the determination of cholera enterotoxin is proposed. This method is based on the selective sorption of the toxin on ganglioside-containing magnetic granules with its subsequent detection by means of immune serum and antispecific immunoperoxidase conjugate. The proposed method permits the detection of 0.052 +/- 0.02 ng of protein of the purified toxin.
Subject(s)
Enterotoxins/analysis , Gangliosides , Magnetics , Vibrio cholerae , Acrylic Resins , Cholera Toxin/analysis , Enzyme-Linked Immunosorbent Assay , Gels , Immunoenzyme Techniques/instrumentation , Immunosorbents , Skin TestsABSTRACT
The quantitative immunofluorescent assay for the determination of cholera enterotoxin is proposed. The assay is based on the selective sorption of cholera enterotoxin by gangliosides incorporated into polyacrylamide granules. The preliminary treatment of gangliosides with neuraminidase enhances the sensitivity of this assay. The assay permits the detection of cholerigen in an amount of 20 ng.
Subject(s)
Enterotoxins/analysis , Vibrio cholerae/analysis , Fluorescent Antibody TechniqueABSTRACT
In this work simple techniques for obtaining polyacrylamide sorbents with magnetic properties are described. These techniques have permitted obtaining block and microgranulated sorbents with the immobilization of antibodies from plague antiserum in the cellular gel structure for the specific sorption of killed and live Yersinia pestis cells and their first fraction; pig brain gangliosides have also been incorporated into the gel structure with a view to the sorption of cholera toxin from the filtrate of Vibrio cholerae culture. The magnetic properties of sorbents, obtained by the copolymerization of powdered magnetic ferric oxides in gel, have made it possible to increase the effectiveness of specific sorption due to mixing and rapid separation in different magnetic fields, as well as to facilitate and accelerate manipulations with the sorbent at all stages. The capacity of different types of sorbents and the time of sorption have been determined.
