[Clinical and morphological characteristics of long-lasting cholestatic syndrome in patients with hepatitis A in the presence of HBV infection]. / Kliniko-morfologicheskaia kharakteristika dlitel'nogo kholestaticheskogo sindroma u bol'nykh gepatitom A na fone nositel'stva HBV-infektsii.

Long-standing cholestatic syndrome (LSCS) was diagnosed in 41 patients with hepatitis A and hepatitis A plus chronic persistent hepatitis B (HA + CPHB). Some patients had a cholestatic form of the disease. Morphological and morphometric liver parameters were measured at the height of icterus in 74 patients. It was found that cholestatic syndrome in patients with HA and HA = CPHB is related to reparative regeneration which always has a favourable outcome.

[Clinico-morphological characteristics of intrahepatic cholestasis in hepatitis A and delta infection]. / Kliniko-morfologicheskie osobennosti vnutripechenochnogo cholestaza pri virusnykh gepatitakh A i del'ta.

Clinical and morphological characteristics were studied for a course of acute viral hepatitis A and delta with long-term cholestatic syndrome in asymptomatic carriers of HBsAg. 149 patients with moderate disease were examined, 53 of them had long-standing cholestasis syndrome. 96 viral hepatitis patients free of cholestasis served control. It is believed that the emergence of long-term intrahepatic cholestasis may be due to the following mechanisms of sinusoid lumina as a result of a drastic enlargement of multinuclear hepatocytes, hyperplasia and hypertrophy of Kupffer's cells. impaired binding of bilirubin in zone III of hepatic acinus. In cholestatic form of hepatitis A there occurs more pronounced reduction of sinusoid volume compared to other variants of viral hepatitis run.

[The possibility of using outbred mice for the production of monoclonal antibodies]. / Vozmozhnost' ispol'zovaniia besporodnykh myshei dlia polucheniia monoklonal'nykh antitel.

Outbred laboratory mice, pretreated with an ascites-forming stimulator and an irradiation dose of 6 Gy, can be used for the production of monoclonal antibodies. However, the passage of hybridomas in irradiated outbred mice rapidly leads to the death of the cells. The properties of antibodies obtained from ascitic fluid of outbred mice are no different from those of antibodies obtained from BALB/c mice.

[Karyological analysis of hybridoma cells after prolonged cultivation]. / Kariologicheskii analiz dlitel'no kul'tiviruemykh gibridomnykh kletok.

The karyological properties, the level of monoclonal antibody production and the proliferative properties of hybridoma strains after their prolonged passage in vivo and in vitro have been studied. Hybridoma EKO-G-2 having the supertetraploid set of chromosomes has proved to be a more stable antibody producer and to possess better proliferative properties. The suggestion has been made that the stability of antibody production is linked with the surplus number of chromosomal copies.

[Passage of hybridomas in male BALB/c mice]. / Passirovanie gibridom na samtsakh myshei linii BALB/c.

For cultivating hybridomas in the ascitic form there are usually used female mice BALB/c and not male ones. Efficiency of production of monoclonal antibodies with cultivation of the hybridomas in male and female mice BALB/c was studied comparatively. The animals were stimulated to form ascite by administration of the incomplete Freund's adjuvant or 3 per cent peptone with petrolatum oil. Some parameters of the ascite formation were studied: viability of the hybridoma cells, ascitic fluid formation period and volume, hybridoma cell concentration and titers of monoclonal antibodies in the ascitic fluid. In regard to all the parameters studied the male animals were not inferior to the female ones and in case of one of the hybridomas even surpassed them twofold by the volume of the ascitic fluid formed. This is evident of possible using male mice for mass cultivation of hybridoma cells with a purpose of obtaining preparative amounts of monoclonal antibodies in production of immunodiagnostic agents on their basis.

[Comparison of the action of pristan and Freund's incomplete adjuvant on the development of ascites in mice--the recipients of hybridoma cells]. / Sravnenie deistviia Pristana i nepolnogo ad''iuvanta Freinda na formirovanie astsita u myshei--retsipientov gibridomnykh kletok.

Production of immunodiagnostic preparations based on monoclonal antibodies requires large amounts of such antibodies. Most frequently preparative quantities of monoclonal antibodies are provided by ascitic fluid from hybridoma-carrying mice. Prior to intraperitoneal administration of hybridoma cells mice are subjected to stimulation with pristan, a mineral oil component. The authors showed that the Freund's incomplete adjuvant (FIA) may be as well used for this purpose. The effect of pristan and the FIA on ascites development in mice with hybridomas producing monoclonal antibodies to the Ia-like antigens of man was studied. The stimulants were administered in amounts of 0.5 ml per a mouse. It was shown that irrespective of the stimulant injection time and the number of the administered hybridoma cells the amount of the ascitic fluid formed in female mice BALB/c stimulated with the FIA was 1.5-3 times higher as that in the animals stimulated with Pristane, the antibody titer in the ascitic fluid being unchanged.

[Vaseline-peptone mixture as a stimulant of ascites formation in mouse hybridoma carriers]. / Vazelin-peptonovaia smes' kak stimuliator obrazovaniia astsita u myshei-nositelei gibridom.

A preliminary injection of vaseline oil with 3% of peptone in a dose of 0.5 ml induced ascitic tumors with a high taking rate in mice used as recipients of hybridomas. The dependence of the amount of ascitic fluid produced in such animals and the content of monoclonal antibodies on the time of the preliminary injection of the above-mentioned mixture, the number of cells introduced into the recipient animal, and the time of ascites collection has been studied. The optimal scheme of obtaining ascites with the use of the proposed mixture is presented. The comparison of the mixture with other stimulants of ascites production has been made.

[Electron microscopic diagnosis of viral diseases]. / Elektronno-mikroskopicheskaia diagnostika virusnykh zabolevanii.

Rotaviruses were detected in 58 among 194 children with nonbacterial gastroenteritis whose feces were examined by direct electron microscopy and immune electron microscopy (IEM). Identification of Coxsackie B viruses isolated from patients with infectious-allergic myocarditis by IEM and the neutralization tests gave similar results (Coxsackie B6). Besides, IEM detected the accompanying virus types.

[ICO-1 monoclonal antibodies against the constant region of Ia-like (Dr) antigens]. / Monoklonal'nye antitela IKO-1 protiv konstantnoi chasti Ia-podobnykh (Dr) antigenov.

Mouse monoclonal antibodies ICO-1 to constant part of Ia-Like (Dr) antigens were produced. Hybridoma continuously produced IgG3 antibodies during 22 passages in vivo, more than 7 months in vitro. Antibodies specifically bound to 29.1 +/- 2.3% of peripheral blood mononuclear cells of healthy people recognized the antigen on B lymphocytes and 44.2 +/- 3.4% of monocytes. This antigen was absent on granulocytes and T lymphocytes. Using monoclonal antibodies ICO-1 antigenically positive cells were detected in 40 patients with B-CLL, in 12 of 35 patients (34.3%) with chronic granulocytic leukemia at the stage of blastic crisis, in 43 of 65 patients (66.1%) with ALL, in 14 of 38 patients (36.8%) with lymphosarcoma and in 17 of 30 patients with acute myelomonocytic leukemia. The antibodies responded to surface antigens in the reaction of indirect surface immunofluorescence, complement-dependent cytotoxic reaction and radioimmune tests.

[Electron microscopic study of the interaction of shigellae with cell cultures. III. Functional-structural changes in transplanted amniotic cells exposed to extracellular Flexner shigellae]. / Elektronno-mikroskopicheskoe izuchenie vzaimodeistviia shigell s kletochnymi kul'turami. III. Funktsional'no-strukturnye izmeneniia perevivaemykh amnioticheskikh kletok pri deistvii vnekletochno raspolozhennykh shigell Fleksnera.

A quantitative changes of RNA and proteins and structural peculiarities of compensative-regenerative character were discovered in amnion cells subjected to the action of extracellular virulent shigellas by means of successive cytophotometry and electron microscopy. The long contact with the bacteria lead to increased degenerative changes in the cell cytoplasm. The arising changes seem to involve toxicity caused by the action of exometabolites of Shigella flexneri.

[An electron microscopic study of the interaction between shigellae and cell cultures. II. Structuro-functional response of the chief cytoplasmic organoids of infected cells]. / Elektronnomikroskopicheskoe izuchenie vzaimodeistviia shigell s kletochnymi kul'turami. II. Strukturno-funktsional'naia reaktsiia osnovnykh organoidov tsitoplazmy infitsirovannykh kletok

The initial mobilization of the protective power of amniotic cells under the influence of the intracellular parasite Shigellae Flexner was shown using qualitative and quantitative methods of electron microscopy. This mobilization is seen in the appearance and then concentration of membrane elements of the endoplasmic reticulum around the bacteria. The character of some degenerating changes confirms the opinion about the presence of an excreting endotoxin fraction in Shigellae Flexner.