ABSTRACT
Two physiologically active oligosaccharide fractions were isolated from pectin of Pisum sativum L. cell wall after its partial acid hydrolysis. These fractions displayed stimulating and inhibiting effects on root formation in thin-layer explants. The subsequent separation of these fractions by gel permeation and anion-exchange chromatography resulted in fractions with effective concentrations two orders of magnitude lower than the concentrations of the initial fractions. The resulting oligosaccharides displayed their effect on the earliest stage of the rhizogenesis associated with formation of root primordias. The rhizogenesis-inhibiting fraction suppressed cell division by 30-50%. The stimulating fraction mainly contained fragments of xyloglucan and galactan, and the inhibiting fraction contained fragments of xyloglucan, galactan, and arabinan. The polymerization degrees of the stimulating and of the inhibiting oligosaccharides were 10-11 and 5-6, respectively.
Subject(s)
Oligosaccharides/pharmacology , Pectins/pharmacology , Pisum sativum/chemistry , Plant Roots/drug effects , Oligosaccharides/isolation & purification , Pectins/isolation & purification , Plants/chemistryABSTRACT
Influence of a single impulse of magnetic field (MF) of trapezoidal shape with 10 Oe amplitude on neuron electrical activity (EA) of Lymnaea stagnalis was studied. It has been found that both leading and trailing edges of MF impulse separately were able to change EA of the cells. Nevertheless the impulses with a short time between the edges were found to be of small efficiency. It was suggested that the influence of the rotation electric field induced by the leading edge of this pulse was abolished by the influence of that induced by the trailing edge. The efficiency of MF impulse influence increased with increasing the interval between their edges and ran to the maximum during 10 s interval.
