ABSTRACT
The schemes for profiles of migration of RNA segments from human rotaviruses isolated in Gorky city in 1981-1987 are presented. The groups of the "long", "short" and "wide" electrophoretypes were identified. The dominant rotaviral phoretypes are determined. The possibilities of electrophoretyping and prospects of the future research are discussed.
Subject(s)
Genes, Viral , RNA, Viral/analysis , Rotavirus/genetics , Electrophoresis, Polyacrylamide Gel , HumansABSTRACT
Rotaviruses were detected in 58 among 194 children with nonbacterial gastroenteritis whose feces were examined by direct electron microscopy and immune electron microscopy (IEM). Identification of Coxsackie B viruses isolated from patients with infectious-allergic myocarditis by IEM and the neutralization tests gave similar results (Coxsackie B6). Besides, IEM detected the accompanying virus types.
Subject(s)
Coxsackievirus Infections/diagnosis , Rotavirus Infections/diagnosis , Antigen-Antibody Complex/analysis , Child, Preschool , Coxsackievirus Infections/microbiology , Enterovirus B, Human/immunology , Enterovirus B, Human/ultrastructure , Feces/microbiology , Humans , Immunologic Techniques , Infant , Infant, Newborn , Intestinal Diseases/diagnosis , Intestinal Diseases/microbiology , Microscopy, Electron , Myocarditis/diagnosis , Myocarditis/microbiology , Rotavirus/immunology , Rotavirus/ultrastructure , Rotavirus Infections/microbiologyABSTRACT
The materials on the epidemiological supervision of poliomyelitis in a large city, carried out in 1972-1981, are presented. Two periods differing in morbidity rate, in the level of population immunity to poliomyelitis and in the activity of the poliovirus circulation are defined. The supervision of poliomyelitis is shown to be capable of influencing the course of the epidemic process of this infection provided that the individual elements of such supervision are correctly evaluated in different epidemic situations.
Subject(s)
Poliomyelitis/epidemiology , Antibodies, Viral/analysis , Antibody Formation , Child , Child, Preschool , Epidemiologic Methods , Feces/microbiology , Humans , Infant , Neutralization Tests , Pharynx/microbiology , Poliomyelitis/microbiology , Poliomyelitis/prevention & control , Poliovirus/immunology , Poliovirus/isolation & purification , Russia , Urban PopulationABSTRACT
The clinical virological survey of 180 cardiological patients aged 16-60 years with clinically diagnosed infectious allergic myocarditis (100), rheumatism (38) and other cardiac diseases (42) was made. A group of 97 practically healthy adults aged 21-60 years was used for control. The survey revealed statistically significant differences in the frequency of occurrence of Coxsackie B virus in healthy persons and in cardiac patients. Thus the criteria of the diagnostic significance of the results of the serologic survey gave grounds for suspicion that diseases in 31.2% of the surveyed patients were connected with the presence of Coxsackie B virus infection. Significantly more frequently Coxsackie B virus infection (predominantly type 4 virus) was found to be associated with infectious allergic myocarditis (42%).