Growth Light Quality Influences Leaf Surface Temperature by Regulating the Rate of Non-Photochemical Quenching Thermal Dissipation and Stomatal Conductance.

Significant efforts have been made to optimise spectrum quality in indoor farming to maximise artificial light utilisation and reduce water loss. For such an improvement, green (G) light supplementation to a red-blue (RB) background was successfully employed in our previous studies to restrict both non-photochemical quenching (NPQ) and stomatal conductance (gs). At the same time, however, the downregulation of NPQ and gs had the opposite influence on leaf temperature (Tleaf). Thus, to determine which factor plays the most prominent role in Tleaf regulation and whether such a response is temporal or permanent, we investigated the correlation between NPQ and gs and, subsequently, Tleaf. To this end, we analysed tomato plants (Solanum lycopersicum L. cv. Malinowy Ozarowski) grown solely under monochromatic LED lamps (435, 520, or 662 nm; 80 µmol m-2 s-1) or a mixed RGB spectrum (1:1:1; 180 µmol m-2 s-1) and simultaneously measured gs and Tleaf with an infrared gas analyser and a thermocouple or an infrared thermal camera (FLIR) during thermal imaging analyses. The results showed that growth light quality significantly modifies Tleaf and that such a response is not temporal. Furthermore, we found that the actual adaxial leaf surface temperature of plants is more closely related to NPQ amplitude, while the temperature of the abaxial surface corresponds to gs.

Effects of partial replacement of red by green light in the growth spectrum on photomorphogenesis and photosynthesis in tomato plants.

The artificial light used in growth chambers is usually devoid of green (G) light, which is considered to be less photosynthetically efficient than blue (B) or red (R) light. To verify the role of G light supplementation in the spectrum, we modified the RB spectrum by progressively replacing R light with an equal amount of G light. The tomato plants were cultivated under 100 µmol m-2 s-1 of five different combinations of R (35-75%) and G light (0-40%) in the presence of a fixed proportion of B light (25%) provided by light-emitting diodes (LEDs). Substituting G light for R altered the plant's morphology and partitioning of biomass. We observed a decrease in the dry biomass of leaves, which was associated with increased biomass accumulation and the length of the roots. Moreover, plants previously grown under the RGB spectrum more efficiently utilized the B light that was applied to assess the effective quantum yield of photosystem II, as well as the G light when estimated with CO2 fixation using RB + G light-response curves. At the same time, the inclusion of G light in the growth spectrum reduced stomatal conductance (gs), transpiration (E) and altered stomatal traits, thus improving water-use efficiency. Besides this, the increasing contribution of G light in place of R light in the growth spectrum resulted in the progressive accumulation of phytochrome interacting factor 5, along with a lowered level of chalcone synthase and anthocyanins. However, the plants grown at 40% G light exhibited a decreased net photosynthetic rate (Pn), and consequently, a reduced dry biomass accumulation, accompanied by morphological and molecular traits related to shade-avoidance syndrome.

Light Quality-Dependent Regulation of Non-Photochemical Quenching in Tomato Plants.

Photosynthetic pigments of plants capture light as a source of energy for photosynthesis. However, the amount of energy absorbed often exceeds its utilization, thus causing damage to the photosynthetic apparatus. Plants possess several mechanisms to minimize such risks, including non-photochemical quenching (NPQ), which allows them to dissipate excess excitation energy in the form of harmless heat. However, under non-stressful conditions in indoor farming, it would be favorable to restrict the NPQ activity and increase plant photosynthetic performance by optimizing the light spectrum. Towards this goal, we investigated the dynamics of NPQ, photosynthetic properties, and antioxidant activity in the leaves of tomato plants grown under different light qualities: monochromatic red (R), green (G), or blue (B) light (L) at 80 µmol m-2 s-1 and R:G:B = 1:1:1 (referred to as the white light (WL)) at 120 µmol m-2 s-1. The results confirm that monochromatic BL increased the quantum efficiency of PSII and photosynthetic pigments accumulation. The RL and BL treatments enhanced the NPQ amplitude and showed negative effects on antioxidant enzyme activity. In contrast, plants grown solely under GL or WL presented a lower amplitude of NPQ due to the reduced accumulation of NPQ-related proteins, photosystem II (PSII) subunit S (PsbS), PROTON GRADIENT REGULATION-LIKE1 (PGRL1), cytochrome b6f subunit f (cytf) and violaxanthin de-epoxidase (VDE). Additionally, we noticed that plants grown under GL or RL presented an increased rate of lipid peroxidation. Overall, our results indicate the potential role of GL in lowering the NPQ amplitude, while the role of BL in the RGB spectrum is to ensure photosynthetic performance and photoprotective properties.