ABSTRACT
A method for the large scale preparation of partially desialylated human chorionic gonadotrophin suitable for human use is reported. To obtain the desired grade of desialylation and to avoid the presence of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. The preparation showed to be active in vitro (OAAD and SVW tests) and its half-life was found to be 13 min in the rat and 75 min in human beings. This desialo hCG proved to be effective in inducing ovulation in amenorrhoeic women. Among 39 induced cycles 31 ovulations and 5 pregnancies occurred.
Subject(s)
Amenorrhea/drug therapy , Asialoglycoproteins , Chorionic Gonadotropin/pharmacology , Menotropins/pharmacology , Ovary/drug effects , Ovulation/drug effects , Adult , Animals , Chorionic Gonadotropin/isolation & purification , Estradiol/blood , Female , Humans , Neuraminidase , Pregnancy , Progesterone/blood , Rats , Sepharose , Sialic Acids/analysis , UltrafiltrationABSTRACT
The effect of the acute administration of three serotonin antagonists on plasma PRL levels and on the PRL response to suckling was investigated in a group of puerperal women. A single oral dose of metergoline or methysergide induced a significant decrease of plasma PRL levels and abolished the PRL response to suckling. Cyproheptadine administration did not modify either the plasma PRL levels or the PRL response to suckling. These results are discussed in light of the known pharmacological properties of the three antiserotonergic drugs.
Subject(s)
Cyproheptadine , Ergolines , Lactation , Metergoline , Methysergide , Postpartum Period , Serotonin Antagonists , Female , Humans , Kinetics , Pregnancy , Prolactin/bloodABSTRACT
Ten patients with hypothalamic anovulation weretreated with a "retard" preparation of synthetic luteinizing hormone releasing hormone (LHRH) after an HMG stimulation in order to induce ovulation and pregnancy. Four of the patient ovulated after intramuscular administration of the LHRH preparation. This study suggests that is is possible to induce ovulation with LHRH in patients pretreated with HMG, and that LHRH has advantages over HCG since it does not induce hyperstimulation even in the presence of exagerated follicular growth. Nevertheless, the optimal conditions for the use and monitoring of LHRH treatment have yet to be clarified.
