ABSTRACT
The presence of human papillomavirus (HPV) DNA in esophageal brushings from human immunodeficiency virus (HIV)-seropositive hosts was investigated in a cross-sectional study. Oral and esophageal brushings from individuals scheduled for esophagogastroscopy (53 HIV-positive and 61 age-matched HIV-negative patients) were tested for the presence of HPV DNA by a consensus L1 polymerase chain reaction assay. HPV DNA was detected in esophageal brushings of 9 (17%) of the 53 HIV-seropositive patients and 0 of the 61 HIV-negative individuals. HPV-16 DNA was the most frequently detected. No proliferative mucosal lesion was noted in individuals with HPV-positive esophageal brushings. Cytological smears were done for 6 of the 9 patients with HPV-positive esophageal brushings, and epithelial atypia was recorded for 1. HIV infection and a history of genital herpes were strong independent predictors of HPV, suggesting that HPV is transmitted sexually in the esophagus.
Subject(s)
AIDS-Related Opportunistic Infections/virology , Capsid Proteins , Esophagus/virology , Papillomaviridae/isolation & purification , Papillomavirus Infections/virology , Tumor Virus Infections/virology , AIDS-Related Opportunistic Infections/pathology , Cross-Sectional Studies , Esophagus/pathology , Female , Humans , Male , Oncogene Proteins, Viral/genetics , Papillomaviridae/genetics , Papillomavirus Infections/pathology , Risk Factors , Tumor Virus Infections/pathologyABSTRACT
BACKGROUND AND OBJECTIVES: To investigate in a cross-sectional study the determinants of oral human papillomavirus infection in 287 individuals who are sexually active. GOAL: To assess prevalence as well as risk factors for oral human papillomavirus infection. STUDY DESIGN: One hundred seventy-eight human immunodeficiency virus-seropositive (158 men and 20 women) and 109 human immunodeficiency virus-negative (73 men and 36 women) individuals were recruited consecutively from sexually transmitted disease-human immunodeficiency virus clinics and gastrointestinal endoscopy clinics. Oral brushings were tested with the L1 consensus polymerase chain reaction assay for human papillomavirus detection. RESULTS: Human papillomavirus DNA was detected in 32 (11.2%) of 287 individuals. Associated with oral human papillomavirus infection on univariate analyses were human immunodeficiency virus infection (odds ratio, 6.9; 95% confidence interval, 2.0-23.2), homosexuality (odds ratio, 3.7; 95% confidence interval, 1.5-9.4), unprotected oral sex (odds ratio, 5.5; 95% confidence interval, 1.6-18.4), syphilis (odds ratio, 2.5; 95% confidence interval, 1.1-6.3), gonorrhea (odds ratio, 4.2; 95% confidence interval, 1.9-9.1), Chlamydia trachomatis (odds ratio, 4.4; 95% confidence interval, 1.8-10.6), and genital herpes (odds ratio, 2.9; 95% confidence interval, 1.3-6.5). Human immunodeficiency virus infection and C. trachomatis were independently predictive of human papillomavirus infection in multivariate stepwise logistic regression.
Subject(s)
HIV Seropositivity/complications , Mouth Diseases/etiology , Papillomaviridae , Papillomavirus Infections/etiology , Sexually Transmitted Diseases/etiology , Tumor Virus Infections/etiology , Adolescent , Adult , Analysis of Variance , Case-Control Studies , Cross-Sectional Studies , Female , Humans , Logistic Models , Male , Middle Aged , Predictive Value of Tests , Prevalence , Risk FactorsABSTRACT
The role of human papillomaviruses (HPVs) in ovarian epithelium (either normal or neoplastic) remains controversial. We have investigated by PCR the presence of HPV DNA in fresh tissue from ovarian neoplasms and in primary cell cultures established from either normal, benign or malignant ovarian surface epithelia. None of the fresh samples and primary ovarian cultures contained HPV DNA sequences.
