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1.
Pain ; 153(6): 1320-1331, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22525520

ABSTRACT

Serotonin is critically involved in neuropathic pain. However, its role is far from being understood owing to the number of cellular targets and receptor subtypes involved. In a rat model of neuropathic pain evoked by chronic constriction injury (CCI) of the sciatic nerve, we studied the role of 5-HT(2B) receptor in dorsal root ganglia (DRG) and the sciatic nerve. We showed that 5-HT(2B) receptor activation both prevents and reduces CCI-induced allodynia. Intrathecal administration of 5-HT(2B) receptor agonist BW723C86 significantly attenuated established mechanical and cold allodynia; this effect was prevented by co-injection of RS127445, a selective 5-HT(2B) receptor antagonist. A single application of BW723C86 on the sciatic nerve concomitantly to CCI dose-dependently prevented mechanical allodynia and significantly reduced cold allodynia 17 days after CCI. This behavioral effect was accompanied with a marked decrease in macrophage infiltration into the sciatic nerve and, in the DRG, with an attenuated abnormal expression of several markers associated with local neuroinflammation and neuropathic pain. CCI resulted in a marked upregulation of 5-HT(2B) receptor expression in sciatic nerve and DRG. In the latter structure, it was biphasic, consisting of a transient early increase (23-fold), 2 days after the surgery and before the neuropathic pain emergence, followed by a steady (5-fold) increase, that remained constant until pain disappeared. In DRG and sciatic nerve, 5-HT(2B) receptors were immunolocalized on sensory neurons and infiltrating macrophages. Our data reveal a relationship between serotonin, immunocytes, and neuropathic pain development, and demonstrate a critical role of 5-HT(2B) receptors in blood-derived macrophages.


Subject(s)
Indoles/pharmacology , Neuralgia/drug therapy , Neuralgia/physiopathology , Receptor, Serotonin, 5-HT2B/metabolism , Serotonin 5-HT2 Receptor Agonists/pharmacology , Thiophenes/pharmacology , Animals , Disease Models, Animal , Ganglia, Spinal/physiology , Male , Neuralgia/immunology , Nociceptors/drug effects , Nociceptors/physiology , Rats , Rats, Sprague-Dawley , Receptor, Serotonin, 5-HT2B/genetics , Sciatic Nerve/physiology , Sciatic Neuropathy/drug therapy , Sciatic Neuropathy/immunology , Sciatic Neuropathy/physiopathology , Serotonin/physiology
2.
Neurogenetics ; 11(4): 441-8, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20593214

ABSTRACT

Hereditary spastic paraplegia (HSP) with thin corpus callosum (TCC) and mental impairment is a frequent subtype of complicated HSP, often inherited as an autosomal recessive (AR) trait. It is clear from molecular genetic analyses that there are several underlying causes of this syndrome, with at least six genetic loci identified to date. However, SPG11 and SPG15 are the two major genes for this entity. To map the responsible gene in a large AR-HSP-TCC family of Tunisian origin, we investigated a consanguineous family with a diagnosis of AR-HSP-TCC excluded for linkage to the SPG7, SPG11, SPG15, SPG18, SPG21, and SPG32 loci. A genome-wide scan was undertaken using 6,090 SNP markers covering all chromosomes. The phenotypic presentation in five patients was suggestive of a complex HSP that associated an early-onset spastic paraplegia with mild handicap, mental deterioration, congenital cataract, cerebellar signs, and TCC. The genome-wide search identified a single candidate region on chromosome 9, exceeding the LOD score threshold of +3. Fine mapping using additional markers narrowed the candidate region to a 45.1-Mb interval (15.4 cM). Mutations in three candidate genes were excluded. The mapping of a novel AR-HSP-TCC locus further demonstrates the extensive genetic heterogeneity of this condition. We propose that testing for this locus should be performed, after exclusion of mutations in SPG11 and SPG15 genes, in AR-HSP-TCC families, especially when cerebellar ataxia and cataract are present.


Subject(s)
Chromosomes, Human, Pair 9 , Corpus Callosum/pathology , Spastic Paraplegia, Hereditary/genetics , Child , Child, Preschool , Chromosome Mapping , Family Health , Female , Genes, Recessive , Genetic Linkage , Humans , Male , Models, Genetic , Pedigree , Polymorphism, Single Nucleotide , Tunisia
3.
PLoS Biol ; 8(6): e1000408, 2010 Jun 29.
Article in English | MEDLINE | ID: mdl-20613862

ABSTRACT

DNA repair is essential to maintain genome integrity, and genes with roles in DNA repair are frequently mutated in a variety of human diseases. Repair via homologous recombination typically restores the original DNA sequence without introducing mutations, and a number of genes that are required for homologous recombination DNA double-strand break repair (HR-DSBR) have been identified. However, a systematic analysis of this important DNA repair pathway in mammalian cells has not been reported. Here, we describe a genome-scale endoribonuclease-prepared short interfering RNA (esiRNA) screen for genes involved in DNA double strand break repair. We report 61 genes that influenced the frequency of HR-DSBR and characterize in detail one of the genes that decreased the frequency of HR-DSBR. We show that the gene KIAA0415 encodes a putative helicase that interacts with SPG11 and SPG15, two proteins mutated in hereditary spastic paraplegia (HSP). We identify mutations in HSP patients, discovering KIAA0415/SPG48 as a novel HSP-associated gene, and show that a KIAA0415/SPG48 mutant cell line is more sensitive to DNA damaging drugs. We present the first genome-scale survey of HR-DSBR in mammalian cells providing a dataset that should accelerate the discovery of novel genes with roles in DNA repair and associated medical conditions. The discovery that proteins forming a novel protein complex are required for efficient HR-DSBR and are mutated in patients suffering from HSP suggests a link between HSP and DNA repair.


Subject(s)
DNA Repair , Genome, Human , RNA Interference , Spastic Paraplegia, Hereditary/genetics , Gene Knockdown Techniques , Humans , Recombination, Genetic
5.
Brain ; 132(Pt 6): 1589-600, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19439420

ABSTRACT

Thirty-four different loci for hereditary spastic paraplegias have been mapped, and 16 responsible genes have been identified. Autosomal recessive forms of spastic paraplegias usually have clinically complex phenotypes but the SPG5, SPG24 and SPG28 loci are considered to be associated with 'pure' forms of the disease. Very recently, five mutations in the CYP7B1 gene, encoding a cytochrome P450 oxysterol 7-alpha hydroxylase and expressed in brain and liver, have been found in SPG5 families. We analysed the coding region and exon-intron boundaries of the CYP7B1 gene by direct sequencing in a series of 82 unrelated autosomal recessive hereditary spastic paraplegia index patients, manifesting either a pure (n = 52) or a complex form (n = 30) of the disease, and in 90 unrelated index patients with sporadic pure hereditary spastic paraplegia. We identified eight, including six novel, mutations in CYP7B1 segregating in nine families. Three of these mutations were nonsense (p.R63X, p.R112X, p.Y275X) and five were missense mutations (p.T297A, p.R417H, p.R417C, p.F470I, p.R486C), the last four clustering in exon 6 at the C-terminal end of the protein. Residue R417 appeared as a mutational hot-spot. The mean age at onset in 16 patients was 16.4 +/- 12.1 years (range 4-47 years). After a mean disease duration of 28.3 +/- 13.4 years (10-58), spasticity and functional handicap were moderate to severe in all cases. Interestingly, hereditary spastic paraplegia was pure in seven SPG5 families but complex in two. In addition, white matter hyperintensities were observed on brain magnetic resonance imaging in three patients issued from two of the seven pure families. Lastly, the index case of one family had a chronic autoimmune hepatitis while his eldest brother died from cirrhosis and liver failure. Whether this association is fortuitous remains unsolved, however. The frequency of CYP7B1 mutations were 7.3% (n = 6/82) in our series of autosomal recessive hereditary spastic paraplegia families and 3.3% (n = 3/90) in our series of sporadic pure spastic paraplegia. The recent identification of CYP7B1 as the gene responsible for SPG5 highlights a novel molecular mechanism involved in hereditary spastic paraplegia determinism.


Subject(s)
Codon, Nonsense/genetics , Mutation, Missense/genetics , Spastic Paraplegia, Hereditary/genetics , Steroid Hydroxylases/genetics , Adolescent , Adult , Aged , Animals , Base Sequence , Brain/pathology , Cytochrome P450 Family 7 , Female , Genes, Recessive , Genetic Variation , Heterozygote , Humans , Magnetic Resonance Imaging , Male , Middle Aged , Pedigree , Point Mutation , Spastic Paraplegia, Hereditary/pathology , Species Specificity , Young Adult
6.
Hum Mutat ; 30(3): E500-19, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19105190

ABSTRACT

Autosomal recessive spastic paraplegia with thinning of corpus callosum (ARHSP-TCC) is a complex form of HSP initially described in Japan but subsequently reported to have a worldwide distribution with a particular high frequency in multiple families from the Mediterranean basin. We recently showed that ARHSP-TCC is commonly associated with mutations in SPG11/KIAA1840 on chromosome 15q. We have now screened a collection of new patients mainly originating from Italy and Brazil, in order to further ascertain the spectrum of mutations in SPG11, enlarge the ethnic origin of SPG11 patients, determine the relative frequency at the level of single Countries (i.e., Italy), and establish whether there is one or more common mutation. In 25 index cases we identified 32 mutations; 22 are novel, including 9 nonsense, 3 small deletions, 4 insertions, 1 in/del, 1 small duplication, 1 missense, 2 splice-site, and for the first time a large genomic rearrangement. This brings the total number of SPG11 mutated patients in the SPATAX collection to 111 cases in 44 families and in 17 isolated cases, from 16 Countries, all assessed using homogeneous clinical criteria. While expanding the spectrum of mutations in SPG11, this larger series also corroborated the notion that even within apparently homogeneous population a molecular diagnosis cannot be achieved without full gene sequencing.


Subject(s)
Agenesis of Corpus Callosum , Gene Deletion , Mutation , Proteins/genetics , Spastic Paraplegia, Hereditary/genetics , Adolescent , Adult , Algeria , Base Sequence , Brazil , DNA Mutational Analysis , Family Health , Female , Gene Frequency , Genes, Recessive , Genetic Testing , Genotype , Haplotypes , Humans , Male , Middle Aged , Morocco , Pedigree , Portugal , Spastic Paraplegia, Hereditary/diagnosis , Spastic Paraplegia, Hereditary/ethnology , Young Adult
7.
J Neurol Sci ; 277(1-2): 22-5, 2009 Feb 15.
Article in English | MEDLINE | ID: mdl-19084844

ABSTRACT

Spastic paraplegia with thinning of the corpus callosum (ARHSP-TCC) is a relatively frequent form of complicated hereditary spastic paraplegia in which mental retardation and muscle stiffness at onset are followed by slowly progressive paraparesis and cognitive deterioration. Although genetically heterogeneous, ARHSP-TCC is frequently associated with mutations in the SPG11 gene, on chromosome 15q. However, it is becoming evident that ARHSP-TCC can also be the clinical presentation of mutations in ZFYVE26 (SPG15), as shown by the recent identification of eight families with a variable phenotype. Here, we present an additional Italian ARHSP-TCC patient harboring two new, probably loss-of-function mutations in ZFYVE26. This finding, together with the report of a mutation in another Italian family, provides confirmation that ZFYVE26 is the second gene responsible for ARHSP-TCC in the Italian population.


Subject(s)
Corpus Callosum/pathology , Paraplegia/genetics , Paraplegia/pathology , Proteins/genetics , Adolescent , Adult , Female , Haplotypes , Humans , Italy , Magnetic Resonance Imaging , Male , Nerve Fibers, Myelinated/pathology , Pedigree , Point Mutation , Young Adult
8.
Arch Neurol ; 65(3): 393-402, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18332254

ABSTRACT

OBJECTIVE: To perform a clinical and genetic study of Tunisian families with autosomal recessive (AR) hereditary spastic paraplegia with thin corpus callosum (HSP-TCC). DESIGN: Linkage studies and mutation screening. SETTING: Reference Center for Neurogenetics in South and Center Tunisia. PARTICIPANTS: Seventy-three subjects from 33 "apparently" unrelated Tunisian families with AR HSP. MAIN OUTCOME MEASURES: Families with AR HSP-TCC were subsequently tested for linkage to the corresponding loci using microsatellite markers from the candidate intervals, followed by direct sequencing of the KIAA1840 gene in families linked to SPG11. RESULTS: We identified 8 Tunisian families (8 of 33 [24%]), including 19 affected patients, fulfilling the clinical criteria for HSP-TCC. In 7 families, linkage to either SPG11 (62.5%) or SPG15 (25%) was suggested by haplotype reconstruction and positive logarithm of odds score values for microsatellite markers. The identification of 2 recurrent mutations (R2034X and M245VfsX) in the SPG11 gene in 5 families validated the linkage results. The neurological and radiological findings in SPG11 and SPG15 patients were relatively similar. The remaining family, characterized by an earlier age at onset and the presence of cataracts, was excluded for linkage to the 6 known loci, suggesting further genetic heterogeneity. CONCLUSIONS: Autosomal recessive HSP-TCC is a frequent subtype of complicated HSP in Tunisia and is clinically and genetically heterogeneous. SPG11 and SPG15 are the major loci for this entity, but at least another genetic form with unique clinical features exists.


Subject(s)
Agenesis of Corpus Callosum , Family Health , Genetic Heterogeneity , Mental Disorders , Proteins/genetics , Spastic Paraplegia, Hereditary , Adult , DNA Mutational Analysis , Female , Genotype , Humans , Magnetic Resonance Imaging/methods , Male , Mental Disorders/complications , Mental Disorders/genetics , Mental Disorders/pathology , Phenotype , Spastic Paraplegia, Hereditary/complications , Spastic Paraplegia, Hereditary/genetics , Spastic Paraplegia, Hereditary/pathology , Tunisia/epidemiology
9.
Brain ; 131(Pt 3): 772-84, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18079167

ABSTRACT

Hereditary spastic paraplegias (HSP) are neurodegenerative diseases mainly characterized by lower limb spasticity associated, in complicated forms, with additional neurological signs. We have analysed a large series of index patients (n = 76) with this condition, either from families with an autosomal recessive inheritance (n = 43) or isolated patients (n = 33), for mutations in the recently identified SPG11 gene. We found 22 truncating mutations, including the first four splice-site mutations, segregating in seven isolated cases and 13 families. Nineteen mutations were novel. Two recurrent mutations were found in Portuguese and North-African patients indicating founder effects in these populations. The mutation frequency varied according to the phenotype, from 41%, in HSP patients presenting with a thin corpus callosum (TCC) visualized by MRI, to 4.5%, in patients with mental impairment without a TCC. Disease onset occurred during the first to the third decade mainly by problems with gait and/or mental retardation. After a mean disease duration of 14.9 +/- 6.6 years, the phenotype of 38 SPG11 patients was severe with 53% of patients wheelchair bound or bedridden. In addition to mental retardation, 80% of the patients showed cognitive decline with executive dysfunction. Interestingly, the phenotype also frequently included lower motor neuron degeneration (81%) with wasting (53%). Slight ocular cerebellar signs were also noted in patients with long disease durations. In addition to a TCC (95%), brain MRI revealed white matter alterations (69%) and cortical atrophy (81%), which worsened with disease duration. In conclusion, our study reveals the high frequency of SPG11 mutations in patients with HSP, a TCC and cognitive impairment, including in isolated patients, and extends the associated phenotype.


Subject(s)
Cognition Disorders/genetics , Corpus Callosum/pathology , Mutation , Proteins/genetics , Spastic Paraplegia, Hereditary/genetics , Adolescent , Adult , Age of Onset , Base Sequence , Brain/pathology , Child , Child, Preschool , Cognition Disorders/pathology , DNA Mutational Analysis/methods , Female , Genes, Recessive , Genetic Linkage , Genotype , Humans , Intellectual Disability/genetics , Intellectual Disability/pathology , Magnetic Resonance Imaging , Male , Molecular Sequence Data , Motor Neuron Disease/genetics , Motor Neuron Disease/pathology , Pedigree , Phenotype , Spastic Paraplegia, Hereditary/pathology , Spastic Paraplegia, Hereditary/psychology
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