Mortality of embryos, developmental disorders and changes in biochemical parameters in marsh frog (Rana ridibunda) tadpoles exposed to the water-soluble fraction of Kazakhstan crude oil and O-Xylene.

The effects of different concentrations of water-soluble fraction of crude oil (WSFO) from the Zhanazhol oil field (Aktobe region, Kazakhstan) and compared to o-xylene, prevalent in this oil, on growth and development of marsh frog (Rana ridibunda) were assessed. In subchronic experiments (7 d), a dose-related increase in mortality and incidence of deformities in embryos were observed. In chronic experiments (60 d; starting from the Gosner stage 26), a dose-dependent decrease in body weight, size and developmental delay by 3-4 stages were also detected. In addition, the content of lipid hyperoxide (LHO) and malondialdehyde (MDA), as well as activities of superoxide dismutase (SOD) and catalase (CAT) enzymes in liver of the tadpoles were determined at the end of chronic experiment. Exposure to 0.5 mg/L or 1.5 mg/L WSFO elevated the content of LHO by 76% and 86%, and MDA by 47% and 58% but decreased SOD activity by 26% and 49%, and CAT by 35% and 46%, respectively. A less pronounced adverse effect was found after chronic exposure to the same concentrations of o-xylene. In tadpole liver exposed to o-xylene levels of LHO was increased by 40% and 51%, MDA by 11% and 29%, while the activity of SOD was lowered by 18% and 41%, and CAT - by 13% and 37% in the 0.5 mg/L and 1.5 mg/L treatment groups, respectively. Data demonstrated the embryotoxic and teratogenic effects attributed to WSFO and o-xylene exposure which may involve oxidative stress mechanisms.

Interaction of stilbene compounds with human and rainbow trout estrogen receptors.

Compounds with stilbene structures are widely used as pharmaceuticals and personal care products (PPCPs) and are present in plants. A suite of stilbene-related compounds, including PPCPs and plant-derived compounds were tested in vitro for interactions with the human and rainbow trout estrogen receptors and in vivo with rainbow trout using vitellogenin levels as a biomarker. Among the compounds with antagonistic activity, the common structural similarity was (in addition to the stilbene backbone) the presence of 4-hydroxy substitution. Stilbene-related compounds found to act as inhibitors at the estrogen receptor included the plant-derived compound resveratrol and two formulations of fluorescent whitening agents used in detergents, 4,4'-bis(2-sulfostyryl)biphenyl and diaminostilbene-1. In the yeast estrogenicity screening assay, the concentrations which caused a 50% inhibition in estrogenic response (IC50s) with the human estrogen receptor ranged from 2.56 x 10(-6) to 2.56 x 10(-6) M. In the rainbow trout estrogen receptor assay, the IC50s ranged from 7.75 x 10(-8) to 1.11 x 10(-5) M. However, in the in vivo rainbow trout vitellogenin assay, tamoxifen was the only stilbene of the compounds tested to have a significant effect as an inhibitor of estrogenicity.