[Modification of the platelet cell membrane and suppression of its function with sodium hypochlorite, detected by optical methods]. / Modifikatsiia plazmaticheskoi membrany trombotsitov i podavlenie ikh funktsii gipokhloritom natriia, obnaruzhivaemye opticheskimi metodami.

The platelet release reaction induced by thrombin was studied by means of the kinetic fluorometric method with usage of the fluorescent probe, acridine orange, and the platelet aggregation was measured with Born's turbidometric method. The release reaction and the arachidonic acid peroxidation are suppressed by sodium hypochlorite at the low concentrations (10-20 mcM) to a greater extent than the platelet aggregation. It is possible that such the inhibitory action of sodium hypochlorite is due to a membrane modification which leads to an alteration of intracellular signalling. Amino groups of platelet plasma membrane estimated by means of the fluorescamine fluorescent label are significantly destroyed at the high concentrations of sodium hypochlorite when the strong inactivation of platelets are observed.

[Molecular mechanisms of the effects of sodium hypochlorite on thrombocytes and lipoproteins]. / Molekuliarnye mekhanizmy deistviia gipokhlorita natriia na trombotsity i lipoproteiny.

Hypochlorite seems to inhibit platelet aggregation in the platelet-rich plasma (PRP) by modifying fibrinogen receptors. The hypochlorite-inactivated isolated platelets are completely repaired by native plasma. Platelet aggregation in PRP is suppressed by hypochlorite by its direct interaction with cells and indirectly due to plasma modification. The indirect action of hypochlorite is a reversible reaction between the platelet active groups and the products of plasma modification. The reaction may involve sulphur-containing groups. The spin-probe method shows that hypochlorite penetrates into the lipid phase of human blood lipoproteins. It initiates lipid peroxidation and causes the disturbance of the lipid structure and the protein please.