[Evaluation of a trophoblastic beta-globulin preparation in the leukocyte adhesion inhibition test during pregnancy]. / Testirovanie preparata trofoblasticheskogo beta-globulina v reaktsii podavleniia prilipaniia leikotsitov pri beremennosti.

The leukocyte adhesion inhibition test was used to study the effect of trophoblastic beta-globulin preparations on the phase of fetal antigen recognition by sensitized lymphocytes from pregnant women. It was found that trophoblastic beta-globulin used in physiological concentrations does not exert an appreciable immunosuppressant effect on pregnant women's lymphocytes both in the first and third trimesters of pregnancy.

[Isolation of placental proteins by means of combined use of adsorption chromatography and immunochemical analysis]. / Sochetanie adsorbtsionnoi khromatografii i immunokhimicheskogo analiza dlia vydeleniia belkov platsenty.

Absorption chromatography was used to isolate antigens from placenta; the most effective purification of the antigens was achieved by selection of suitable eluents. Combined use of adsorption chromatography and immunochemical analyses showed that chorionic alpha1-globulin was isolated most effectively by means of calcium pyrophosphate and placental lactogen on the columns with silica gel and aluminum hydroxide.

[Effect of specific placental proteins--trophoblastic beta 1-glycoprotein chorionic alpha 1-microglobulin--on the proliferation of lymphocytes and malignant fibroblasts in vitro]. / Deistvie spetsificheskikh platsentarnykh belkov--trofoblasticheskogo beta 1-glikoproteida 1 khorionicheskogo al'fa 1-mikroglobulina--na profileratsiiu limfotsitov i zlokachestvennykh fibroblastov in vitro.

A study was made of the action of trophoblastic beta 1-glycoprotein (TBG) and chorionic alpha 1-microglobulin (CAG1) on proliferation of malignant fibroblasts (transplanted L line) and on phytohemagglutinin-stimulated lymphocytes. TBG depressed proliferation of the stimulated lymphocytes and transformed fibroblasts (according to 3H-thymidine incorporation). A dose-response dependence was ascertained. CAG1 did not affect cell division. The inhibitory effect of TBG was seen to be reversed or decreased provided the lymphocyte culture was supplemented with CAG1. The decreased inhibitory effect of TBG in the presence of CAG1 was also noted in the L cell culture. It is likely that in vivo protection of intensely proliferating fetal tissues or tumor from the inhibitors is effected just in this way since placental proteins are synthesized both by embryonic and tumorous cells.

[Physico-chemical properties of trophoblast-specific beta 1-globulin]. / Izuchenie nekotorykh fiziko-khimicheskikh svoistv trofoblast-spetsificheskogo beta 1-globulina.

It has been found that the trophoblast-specific beta 1-globulin [TSG) contains one site of binding to Con A and phytohemagglutinin. The interaction with phytohemagglutinin should be taken into consideration, when studying the immunosuppressive activity of the protein. Additional fractions of the protein with fast anodic electrophoretic mobility and lower molecular weights have been detected after treatment of the trophoblast-specific beta 1-globulin with diethylene triaminopentaacetate. This is indicative of a subunit structure of the glycoprotein molecules.