Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 21
Filter
Add more filters










Publication year range
1.
Sensors (Basel) ; 22(19)2022 Sep 23.
Article in English | MEDLINE | ID: mdl-36236317

ABSTRACT

In this study, we explored the potential of applying biosensors based on silicon nanowire field-effect transistors (bio-NWFETs) as molecular absorption sensors. Using quercetin and Copper (Cu2+) ion as an example, we demonstrated the use of an opto-FET approach for the detection of molecular interactions. We found that photons with wavelengths of 450 nm were absorbed by the molecular complex, with the absorbance level depending on the Cu2+ concentration. Quantitative detection of the molecular absorption of metal complexes was performed for Cu2+ concentrations ranging between 0.1 µM and 100 µM, in which the photon response increased linearly with the copper concentration under optimized bias parameters. Our opto-FET approach showed an improved absorbance compared with that of a commercial ultraviolet-visible spectrophotometry.


Subject(s)
Biosensing Techniques , Coordination Complexes , Nanowires , Copper , Quercetin , Silicon , Transistors, Electronic
2.
Pharmacol Res ; 178: 106148, 2022 04.
Article in English | MEDLINE | ID: mdl-35231572

ABSTRACT

Acquired resistance to vemurafenib (PLX4032) is a thorny issue in BRAFV600E mutant melanoma therapy. Ferroptotic programmed cell death is a potential strategy for combating therapy-resistant cancers. This study uncovers the adaptation and abnormal upregulation of PUFAs and bioactive oxylipin metabolism in PLX4032 resistant melanoma cells. Phyto-sesquiterpene lactone, DET, and its derivative, DETD-35, induced lipid ROS accumulation and triggered ferroptotic cell death in PLX4032 sensitive (A375) and resistant (A375-R) BRAFV600E melanoma cells by reprogramming glutathione and primary metabolisms, lipid/oxylipin metabolism, and causing mitochondrial damage in which DETD-35 showed superior efficiency to DET. We discovered that DET and DETD-35 are a new type of GPX4 enzyme inhibitor through non-covalent binding. This study provides new insight into the therapeutic mechanisms of both DET and DETD-35 to combat PLX4032 sensitive/resistant BRAFV600E mutant melanomas via targeting GPX4 and ferroptosis.


Subject(s)
Ferroptosis , Melanoma , Phospholipid Hydroperoxide Glutathione Peroxidase , Sesquiterpenes , Cell Line, Tumor , Drug Resistance, Neoplasm , Enzyme Inhibitors/pharmacology , Ferroptosis/drug effects , Humans , Indoles/pharmacology , Lactones/pharmacology , Melanoma/drug therapy , Melanoma/genetics , Melanoma/metabolism , Oxylipins/metabolism , Phospholipid Hydroperoxide Glutathione Peroxidase/antagonists & inhibitors , Proto-Oncogene Proteins B-raf/metabolism , Sesquiterpenes/pharmacology , Sulfonamides/pharmacology , Vemurafenib/pharmacology
3.
Acta Crystallogr D Struct Biol ; 75(Pt 12): 1138-1147, 2019 Dec 01.
Article in English | MEDLINE | ID: mdl-31793907

ABSTRACT

The catalytic domain (residues 128-449) of the Orpinomyces sp. Y102 CelC7 enzyme (Orp CelC7) exhibits cellobiohydrolase and cellotriohydrolase activities. Crystal structures of Orp CelC7 and its cellobiose-bound complex have been solved at resolutions of 1.80 and 2.78 Å, respectively. Cellobiose occupies subsites +1 and +2 within the active site of Orp CelC7 and forms hydrogen bonds to two key residues: Asp248 and Asp409. Furthermore, its substrate-binding sites have both tunnel-like and open-cleft conformations, suggesting that the glycoside hydrolase family 6 (GH6) Orp CelC7 enzyme may perform enzymatic hydrolysis in the same way as endoglucanases and cellobiohydrolases. LC-MS/MS analysis revealed cellobiose (major) and cellotriose (minor) to be the respective products of endo and exo activity of the GH6 Orp CelC7.


Subject(s)
Bacterial Proteins/chemistry , Cellobiose/metabolism , Cellulase/chemistry , Cellulose 1,4-beta-Cellobiosidase/chemistry , Cellulose/metabolism , Neocallimastigales/enzymology , Trioses/metabolism , beta-Glucosidase/chemistry , Binding Sites , Crystallography, X-Ray/methods , Models, Molecular , Protein Conformation , Substrate Specificity
4.
Int J Biol Macromol ; 126: 141-150, 2019 Apr 01.
Article in English | MEDLINE | ID: mdl-30586591

ABSTRACT

Oral administration is a highly attractive approach for the delivery of protein drugs. However, oral protein therapeutics typically exhibit extremely poor bioavailability due to the harsh gastrointestinal (GI) environments and low permeability of protein across the intestinal barrier. Trimethyl chitosan (TMC) shows excellent mucoadhesive and absorption-enhancing properties while fucoidan (FD) has hypoglycemic effects and can prevent diabetes-related complications. Here we report, for the first time, that TMC combined with FD can be developed to a mutlifunctional nanoplatform for enhancing the transepithelial permeation of insulin through the intestinal epithelial cell barrier and inhibiting the α-glucosidase activity. TMC and FD self-assembled into spherical nanoparticles (NPs) for insulin encapsulation. TMC/FD NPs protected insulin against degradation by releasing insulin in a pH-dependent manner in the gastrointestinal tract fluids. The NPs were able to modulate the barrier function of the Caco-2 intestinal epithelial cell monolayer, and enhance paracellular transport of insulin across the intestinal barrier. TMC/FD NPs also showed α-glucosidase inhibitory activity, with an inhibition ratio of 33.2% at 2 mg/mL. The superior transepithelial absorption enhancing property of the TMC/FD NPs is expected to combine in the future with the functions of fucoidan against diabetes-related complications for development of advanced mutlifunctional therapeutic platforms for diabetes.


Subject(s)
Chitosan/chemistry , Drug Delivery Systems , Insulin/administration & dosage , Nanoparticles/chemistry , Polysaccharides/chemistry , Administration, Oral , Caco-2 Cells , Cell Death/drug effects , Drug Liberation , Electric Impedance , Glycoside Hydrolase Inhibitors/pharmacology , Humans , Hydrogen-Ion Concentration , Intestines/cytology , Models, Biological , Nanoparticles/ultrastructure , Permeability , Spectroscopy, Fourier Transform Infrared , alpha-Amylases/antagonists & inhibitors , alpha-Amylases/metabolism , alpha-Glucosidases/metabolism
5.
Nanotechnology ; 28(16): 165501, 2017 Apr 21.
Article in English | MEDLINE | ID: mdl-28247853

ABSTRACT

We report on a technique that can extend the use of nanowire sensors to the detection of interactions involving nonpolar and neutral molecules in an ionic solution environment. This technique makes use of the fact that molecular interactions result in a change in the permittivity of the molecules involved. For the interactions taking place at the surface of nanowires, this permittivity change can be determined from the analysis of the measured complex impedance of the nanowire. To demonstrate this technique, histidine was detected using different charge polarities controlled by the pH value of the solution. This included the detection of electrically neutral histidine at a sensitivity of 1 pM. Furthermore, it is shown that nonpolar molecules, such as hexane, can also be detected. The technique is applicable to the use of nanowires with and without a surface-insulating oxide. We show that information about the changes in amplitude and the phase of the complex impedance reveals the fundamental characteristics of the molecular interactions, including the molecular field and the permittivity.

6.
Nanomedicine (Lond) ; 11(1): 9-30, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26654241

ABSTRACT

AIM: Development of epigallocatechin gallate (EGCG) and gelatin-doxorubicin conjugate (GLT-DOX)-coated gold nanoparticles (DOX-GLT/EGCG AuNPs) for fluorescence imaging and inhibition of prostate cancer cell growth. MATERIALS & METHODS: AuNPs alternatively coated with EGCG and DOX-GLT conjugates were prepared by a layer-by-layer assembly method. The physicochemical properties of the AuNPs and the effect of Laminin 67R receptor-mediated endocytosis on the anticancer efficacy of the AuNPs were examined. RESULTS: The AuNPs significantly inhibit the proliferation of PC-3 cancer cell and the enzyme-responsive intracellular release of DOX could be tracked by monitoring the recovery of the fluorescence signal of DOX. CONCLUSION: Laminin 67R receptor-mediated delivery of DOX using the AuNPs enhanced cellular uptake of DOX and improved apoptosis of PC-3 cells.


Subject(s)
Antibiotics, Antineoplastic/administration & dosage , Catechin/analogs & derivatives , Doxorubicin/administration & dosage , Gelatin/chemistry , Gold/chemistry , Metal Nanoparticles , Antibiotics, Antineoplastic/chemistry , Catechin/chemistry , Cell Line, Tumor , Doxorubicin/chemistry , Humans , Male
7.
Acta Crystallogr F Struct Biol Commun ; 71(Pt 10): 1264-72, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26457517

ABSTRACT

Exoglucanase/cellobiohydrolase (EC 3.2.1.176) hydrolyzes a ß-1,4-glycosidic bond from the reducing end of cellulose and releases cellobiose as the major product. Three complex crystal structures of the glycosyl hydrolase 48 (GH48) cellobiohydrolase S (ExgS) from Clostridium cellulovorans with cellobiose, cellotetraose and triethylene glycol molecules were solved. The product cellobiose occupies subsites +1 and +2 in the open active-site cleft of the enzyme-cellotetraose complex structure, indicating an enzymatic hydrolysis function. Moreover, three triethylene glycol molecules and one pentaethylene glycol molecule are located at active-site subsites -2 to -6 in the structure of the ExgS-triethylene glycol complex shown here. Modelling of glucose into subsite -1 in the active site of the ExgS-cellobiose structure revealed that Glu50 acts as a proton donor and Asp222 plays a nucleophilic role.


Subject(s)
Cellulases/chemistry , Cellulases/metabolism , Cellulose/analogs & derivatives , Clostridium cellulovorans/enzymology , Tetroses/metabolism , Biocatalysis , Calcium/metabolism , Catalytic Domain , Cellulose/chemistry , Cellulose/metabolism , Crystallization , Crystallography, X-Ray , Molecular Docking Simulation , Protein Structure, Secondary , Tetroses/chemistry
8.
Bioresour Technol ; 170: 513-521, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25164344

ABSTRACT

A new strain of rumen fungus was isolated from Bos taurus, identified and designated Orpinomyces sp.Y102. A clone, celC7, isolated from the cDNA library of Orpinomyces sp.Y102, was predicted to encode a protein containing a signal peptide (Residues 1-17), an N-terminal dockerin-containing domain, and a C-terminal cellobiohydrolase catalytic domain of glycoside hydrolase family 6. CelC7 was insoluble when expressed in Escherichia coli. Deletion of 17 or 105 residues from the N-terminus significantly improved its solubility. The resulting enzymes, CelC7(-17) and CelC7(-105), were highly active to ß-glucan substrates and were stable between pH 5.0 and 11.0. CelC7(-105) worked as an exocellulase releasing cellobiose and cellotriose from acid-swollen Avicel and cellooligosaccharides, and displayed a Vmax of 6321.64µmole/min/mg and a Km of 2.18mg/ml to barley ß-glucan. Further, the crude extract of CelC7(-105) facilitated ethanol fermentation from cellulose. Thus, CelC7(-105) is a good candidate for industrial applications such as biofuel production.


Subject(s)
Cattle/microbiology , Cellulases/metabolism , Cellulose 1,4-beta-Cellobiosidase/metabolism , Industrial Microbiology/methods , Neocallimastigales/enzymology , Rumen/microbiology , Animals , Base Sequence , Biofuels , Blotting, Western , Cellulases/genetics , Cellulose 1,4-beta-Cellobiosidase/genetics , Chromatography, Thin Layer , Cluster Analysis , DNA Primers/genetics , Escherichia coli , Gene Library , Molecular Sequence Data , Neocallimastigales/cytology , Neocallimastigales/genetics , Phylogeny , Sequence Analysis, DNA
9.
Nano Lett ; 13(6): 2564-9, 2013 Jun 12.
Article in English | MEDLINE | ID: mdl-23634905

ABSTRACT

We argue that the structure ordering of self-assembled probing molecular monolayers is essential for the reliability and sensitivity of nanowire-based field-effect sensors because it can promote the efficiency for molecular interactions as well as strengthen the molecular dipole field experienced by the nanowires. In the case of monolayers, we showed that structure ordering could be improved by means of electrical field alignment. This technique was then employed to align multilayer complexes for nanowire sensing applications. The sensitivity we achieved for detection of hybridization between 15-base single-strand DNA molecules is 0.1 fM and for alcohol sensors is 0.5 ppm. The reliability was confirmed by repeated tests on chips that contain multiple nanowire sensors.


Subject(s)
Electromagnetic Fields , Nanowires , Surface Properties
10.
Bioresour Technol ; 123: 125-34, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22940309

ABSTRACT

Rumen fungi are a rich source of enzymes degrading lignocelluloses. XynR8 is a glycosyl hydrolase family 11 xylanase previously cloned from unpurified rumen fungal cultures. Phylogenetic analysis suggested that xynR8 was obtained from a Neocallimastix species. Recombinant XynR8 expressed in Escherichia coli was highly active and stable between pH 3.0 and 11.0, and displayed a V(max) of 66,672µmolmin(-1)mg(-1), a k(cat) of 38,975s(-1), and a K(m) of 11.20mg/mL towards soluble oat spelt xylan. Based on molecular modeling, residues N41 and N58, important in stabilizing two loops and the structure of XynR8, were mutated to D. Both mutant enzymes showed higher tolerance to pH 2.0. The V(max), k(cat) and K(m) of the N41D and N58D mutant enzymes were 79,645µmolmin(-1)mg(-1), 46,493s(-1), 29.29mg/mL, and 96,689µmolmin(-1)mg(-1), 56,503s(-1), and 21.24mg/mL, respectively. Thus, they are good candidates for application, including biofuel production.


Subject(s)
Endo-1,4-beta Xylanases/chemistry , Endo-1,4-beta Xylanases/metabolism , Models, Molecular , Neocallimastix/enzymology , Rumen/microbiology , Amino Acid Sequence , Animals , Cells, Cultured , Chromatography, Thin Layer , Endo-1,4-beta Xylanases/genetics , Endo-1,4-beta Xylanases/isolation & purification , Enzyme Stability , Fungal Proteins/chemistry , Fungal Proteins/genetics , Fungal Proteins/isolation & purification , Fungal Proteins/metabolism , Hydrogen-Ion Concentration , Hydrolysis , Kinetics , Molecular Sequence Data , Mutagenesis, Site-Directed , Mutant Proteins/chemistry , Mutant Proteins/isolation & purification , Mutant Proteins/metabolism , Phylogeny , Recombinant Proteins/metabolism , Substrate Specificity , Temperature
11.
Biochem Biophys Res Commun ; 407(3): 593-8, 2011 Apr 15.
Article in English | MEDLINE | ID: mdl-21426903

ABSTRACT

In this paper, we determine the mutant W203F structure of TFsß-glucanase, which contains aromatic residue Trp203 at the active site of the enzyme. Residue Trp203 is stacked with the glucose product of cellotriose. Further analysis reveals that two extra calcium ions and a Tris molecule bind to the mutant structure. A Tris molecule, bound to the catalytic residues of Glu56 and Glu60, was found at the position normally taken by substrate binding at the -1 subsite. In addition, a second Ca(2+) ion was found near the residues Phe152 and Glu154 on the protein's surface, and a third one near the active site residue Asp202. Kinetic experiments reveal that both Tris and imidazole are competitive inhibitors, while calcium is a noncompetitive inhibitor for TFsß-glucanase. The two types of enzymatic inhibition are first-time descriptions for the glycosyl hydrolase family 16.


Subject(s)
Calcium/pharmacology , Glycoside Hydrolases/antagonists & inhibitors , Glycoside Hydrolases/chemistry , Tromethamine/pharmacology , Calcium/chemistry , Catalytic Domain/genetics , Cations, Divalent/chemistry , Cations, Divalent/pharmacology , Crystallography, X-Ray , Fibrobacter/enzymology , Glycoside Hydrolases/genetics , Imidazoles/chemistry , Imidazoles/pharmacology , Mutation , Paenibacillus/enzymology , Tromethamine/chemistry , Tryptophan/chemistry , Tryptophan/genetics
12.
Proteins ; 78(13): 2820-30, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20635417

ABSTRACT

We created 12 mutant enzymes (E11L, F40I, Y42L, N44L, N44Q, E47I, L62G, K64A, K64M, R137M, R137Q, and N139A) from the truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (TF-glucanase). The enzymes were used to investigate the structural and catalytic roles of specific amino acid residues located at the catalytic pocket and having direct interactions with glucose subsites of the product beta-1,3-1,4-cellotriose (CLTR). Fluorescence spectrometry showed no discernible changes in secondary structures among purified TF-glucanase and the mutants. Kinetic analyses showed E11L, F40I, Y42L, R137M, and R137Q with a >10-fold decrease of specific activity (11.2- to 67.4-fold), and E11L, N44Q, E47I, K64M, R137M, R137Q, and N139A with a 2.17- to 4.3-fold increase of K(m) value when compared with TF-glucanase. Notably, E11L, R137Q, R137M, F40I, and N139A showed the most significant decrease in catalytic efficiency relative to TF-glucanase, by 2155-, 84.9-, 48.5-, 41.1-, and 19.1-fold, respectively; the five mutants showed the greatest changes in comparative energy DeltaDeltaG(b), with values of 1.94 to 4.92 kcal/mol. Combined with results from kinetic and structure modeling analyses of all mutant enzymes and X-ray crystallography of F40I, we elucidate that Glu11, Phe40, Arg137, and Asn139 play a crucial role in the catalysis of TF-glucanase owing to their local and direct interaction through hydrogen bonds or van der Waals stacking interaction by aromatic rings onto the glucose subsites -3, -2, and -1 of CLTR/substrate. The overall globular structures in the wild-type and mutant F40I enzymes do not differ.


Subject(s)
Amino Acids/chemistry , Bacterial Proteins/chemistry , Fibrobacter/enzymology , Glycoside Hydrolases/chemistry , Amino Acid Sequence , Amino Acids/genetics , Amino Acids/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Binding Sites/genetics , Biocatalysis , Cellulose/chemistry , Cellulose/metabolism , Crystallography, X-Ray , Fibrobacter/genetics , Glycoside Hydrolases/genetics , Glycoside Hydrolases/metabolism , Kinetics , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Mutation , Protein Binding , Protein Conformation , Protein Structure, Secondary , Protein Structure, Tertiary , Sequence Homology, Amino Acid , Spectrometry, Fluorescence , Substrate Specificity , Temperature , Trioses/chemistry , Trioses/metabolism
13.
Biochim Biophys Acta ; 1790(4): 231-9, 2009 Apr.
Article in English | MEDLINE | ID: mdl-19714803

ABSTRACT

BACKGROUND: Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (Fsbeta-glucanase) is the only naturally occurring circularly permuted beta-glucanase among bacterial glucanases with reverse protein domains. We characterized the functional and structural significance of residues 200-209 located in the domain B of Fsbeta-glucanase, corresponding to the major surface loop in the domain A region of Bacillus licheniformis glucanase. METHODS: Rational design approaches including site-directed mutagenesis, initial-rate kinetics, and structural modeling analysis were used in this study. RESULTS: Our kinetic data showed that D202N and D206N exhibited a 1.8- and 1.5-fold increase but G207N, G207-, F205L, N208G and T204F showed a 7.0- to 2.2-fold decrease, in catalytic efficiency (k(cat)/K(M)) compared to the wild-type enzyme. The comparative energy DeltaDeltaG(b) value in individual mutant enzymes was well correlated to their catalytic efficiency. D206R mutant enzyme exhibited the highest relative activity at 50 degrees C over 10 min, whereas K200F was the most heat-sensitive enzyme. CONCLUSIONS: This study demonstrates that Phe205, Gly207, and Asn208 in the Type II turn of the connecting loop may play a role in the catalytic function of Fsbeta-glucanase. GENERAL SIGNIFICANCE: Residues 200-209 in Fsbeta-glucanase resided at the similar structural topology to that of Bacillus enzyme were found to play some similar catalytic function in glucanase.


Subject(s)
Fibrobacter/enzymology , Glycoside Hydrolases/chemistry , Glycoside Hydrolases/metabolism , Amino Acid Sequence , Catalysis , Glycoside Hydrolases/genetics , Hydrogen-Ion Concentration , Kinetics , Models, Molecular , Mutagenesis, Site-Directed , Spectrometry, Fluorescence , Temperature , Thermodynamics
14.
Acta Crystallogr D Biol Crystallogr ; 64(Pt 12): 1259-66, 2008 Dec.
Article in English | MEDLINE | ID: mdl-19018102

ABSTRACT

1,3-1,4-beta-D-Glucanases (EC 3.2.1.73) specifically hydrolyze beta-1,4-glycosidic bonds located prior to beta-1,3-glycosidic linkages in lichenan or beta-D-glucans. It has been suggested that truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (TFsbeta-glucanase) can accommodate five glucose rings in its active site upon enzyme-substrate interaction. In this study, 12 mutant enzymes were created by mutating the conserved residues Gln70, Asn72, Gln81 and Glu85 proposed to bind to substrate subsites +1 and +2 and the catalytic properties of these mutants were determined. The most significant change in catalytic activity was observed on mutation of Gln70, with a 299-fold and 498-fold lower k(cat)/K(m) for the mutants Q70A and Q70I, respectively, compared with the wild-type enzyme. Mutagenesis, kinetic and structural studies revealed that the conserved residues surrounding the active site of TFsbeta-glucanase at substrate subsites +1 and +2 play an important role in its catalytic function, with the following order of importance: Gln70 > Asn72 > Glu85 > Gln81. The crystal structure of mutant E85I was determined at 2.2 A resolution. Further analysis of the E85I mutant structure revealed that the loop located at the concave site moved approximately 2 A from its position in the native enzyme complex without changing the core structure.


Subject(s)
Catalytic Domain/genetics , Fibrobacter/enzymology , Glycoside Hydrolases/metabolism , Mutant Proteins/metabolism , Binding Sites , Calcium Signaling/genetics , Carbohydrates/chemistry , Cloning, Molecular , Crystallization , Glycoside Hydrolases/chemistry , Glycoside Hydrolases/genetics , Mutagenesis, Site-Directed , Mutant Proteins/chemistry , Mutant Proteins/genetics , Protein Conformation , Sequence Deletion , Structure-Activity Relationship , Substrate Specificity
15.
J Comput Aided Mol Des ; 22(12): 915-23, 2008 Dec.
Article in English | MEDLINE | ID: mdl-18663584

ABSTRACT

Glycosyl hydrolase family 16 (GHF16) truncated Fibrobacter succinogenes (TFs) and GHF17 barley 1,3-1,4-beta-D-glucanases (beta-glucanases) possess different structural folds, beta-jellyroll and (beta/alpha)8, although they both catalyze the specific hydrolysis of beta-1,4 glycosidic bonds adjacent to beta-1,3 linkages in mixed beta-1,3 and beta-1,4 beta-D-glucans or lichenan. Differences in the active site region residues of TFs beta-glucanase and barley beta-glucanase create binding site topographies that require different substrate conformations. In contrast to barley beta-glucanase, TFs beta-glucanase possesses a unique and compact active site. The structural analysis results suggest that the tyrosine residue, which is conserved in all known 1,3-1,4-beta-D-glucanases, is involved in the recognition of mixed beta-1,3 and beta-1,4 linked polysaccharide.


Subject(s)
Carbohydrate Metabolism , Endo-1,3(4)-beta-Glucanase/metabolism , Models, Structural , Plants/enzymology , Tyrosine/metabolism , Amino Acid Sequence , Catalytic Domain , Endo-1,3(4)-beta-Glucanase/chemistry , Models, Molecular , Molecular Sequence Data , Sequence Homology, Amino Acid , Substrate Specificity , Tyrosine/chemistry
16.
J Mol Biol ; 354(3): 642-51, 2005 Dec 02.
Article in English | MEDLINE | ID: mdl-16246371

ABSTRACT

Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (Fsbeta-glucanase) catalyzes the specific hydrolysis of beta-1,4 glycosidic bonds adjacent to beta-1,3 linkages in beta-D-glucans or lichenan. This is the first report to elucidate the crystal structure of a truncated Fsbeta-glucanase (TFsbeta-glucanase) in complex with beta-1,3-1,4-cellotriose, a major product of the enzyme reaction. The crystal structures, at a resolution of 2.3 angstroms, reveal that the overall fold of TFsbeta-glucanase remains virtually unchanged upon sugar binding. The enzyme accommodates five glucose residues, forming a concave active cleft. The beta-1,3-1,4-cellotriose with subsites -3 to -1 bound to the active cleft of TFsbeta-glucanase with its reducing end subsite -1 close to the key catalytic residues Glu56 and Glu60. All three subsites of the beta-1,3-1,4-cellotriose adopted a relaxed C(1)4 conformation, with a beta-1,3 glycosidic linkage between subsites -2 and -1, and a beta-1,4 glycosidic linkage between subsites -3 and -2. On the basis of the enzyme-product complex structure observed in this study, a catalytic mechanism and substrate binding conformation of the active site of TFsbeta-glucanase is proposed.


Subject(s)
Cellulose/metabolism , Endo-1,3(4)-beta-Glucanase/chemistry , Endo-1,3(4)-beta-Glucanase/metabolism , Fibrobacter/enzymology , Oligosaccharides/chemistry , Oligosaccharides/metabolism , Trioses/metabolism , Cellulose/chemistry , Crystallography, X-Ray , Endo-1,3(4)-beta-Glucanase/genetics , Fibrobacter/genetics , Models, Molecular , Protein Structure, Quaternary , Protein Structure, Tertiary , Substrate Specificity , Trioses/chemistry
17.
EMBO J ; 22(15): 4014-25, 2003 Aug 01.
Article in English | MEDLINE | ID: mdl-12881435

ABSTRACT

The Vibrio vulnificus nuclease, Vvn, is a non-specific periplasmic nuclease capable of digesting DNA and RNA. The crystal structure of Vvn and that of Vvn mutant H80A in complex with DNA were resolved at 2.3 A resolution. Vvn has a novel mixed alpha/beta topology containing four disulfide bridges, suggesting that Vvn is not active under reducing conditions in the cytoplasm. The overall structure of Vvn shows no similarity to other endonucleases; however, a known 'betabetaalpha-metal' motif is identified in the central cleft region. The crystal structure of the mutant Vvn-DNA complex demonstrates that Vvn binds mainly at the minor groove of DNA, resulting in duplex bending towards the major groove by approximately 20 degrees. Only the DNA phosphate backbones make hydrogen bonds with Vvn, suggesting a structural basis for its sequence-independent recognition of DNA and RNA. Based on the enzyme-substrate and enzyme-product structures observed in the mutant Vvn-DNA crystals, a catalytic mechanism is proposed. This structural study suggests that Vvn hydrolyzes DNA by a general single-metal ion mechanism, and indicates how non-specific DNA-binding proteins may recognize DNA.


Subject(s)
Bacterial Proteins/metabolism , DNA, Bacterial/metabolism , Deoxyribonucleases/metabolism , Esterases/metabolism , Vibrio vulnificus/enzymology , Amino Acid Sequence , Bacterial Proteins/chemistry , Base Sequence , Crystallography, X-Ray , DNA Primers , Deoxyribonucleases/chemistry , Esterases/chemistry , Hydrolysis , Models, Molecular , Molecular Sequence Data , Protein Binding , Protein Conformation , Sequence Homology, Amino Acid , Substrate Specificity
18.
J Mol Biol ; 330(3): 607-20, 2003 Jul 11.
Article in English | MEDLINE | ID: mdl-12842475

ABSTRACT

The 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes (Fsbeta-glucanase) is classified as one of the family 16 glycosyl hydrolases. It hydrolyzes the glycosidic bond in the mixed-linked glucans containing beta-1,3- and beta-1,4-glycosidic linkages. We constructed a truncated form of recombinant Fsbeta-glucanase containing the catalytic domain from amino acid residues 1-258, which exhibited a higher thermal stability and enzymatic activity than the full-length enzyme. The crystal structure of the truncated Fsbeta-glucanase was solved at a resolution of 1.7A by the multiple wavelength anomalous dispersion (MAD) method using the anomalous signals from the seleno-methionine-labeled protein. The overall topology of the truncated Fsbeta-glucanase consists mainly of two eight-stranded anti-parallel beta-sheets arranged in a jellyroll beta-sandwich, similar to the fold of many glycosyl hydrolases and carbohydrate-binding modules. Sequence comparison with other bacterial glucanases showed that Fsbeta-glucanase is the only naturally occurring circularly permuted beta-glucanase with reversed sequences. Structural comparison shows that the engineered circular-permuted Bacillus enzymes are more similar to their parent enzymes with which they share approximately 70% sequence identity, than to the naturally occurring Fsbeta-glucanase of similar topology with 30% identity. This result suggests that protein structure relies more on sequence identity than topology. The high-resolution structure of Fsbeta-glucanase provides a structural rationale for the different activities obtained from a series of mutant glucanases and a basis for the development of engineered enzymes with increased activity and structural stability.


Subject(s)
Bacteria/enzymology , Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Glycoside Hydrolases/chemistry , Glycoside Hydrolases/metabolism , Models, Molecular , Amino Acid Sequence , Bacillus/enzymology , Bacterial Proteins/genetics , Binding Sites , Calcium/metabolism , Catalytic Domain , Crystallography, X-Ray , Glycoside Hydrolases/genetics , Molecular Sequence Data , Protein Conformation , Sequence Homology, Amino Acid , Structural Homology, Protein
19.
Protein Sci ; 11(12): 2947-57, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12441392

ABSTRACT

H-N-H is a motif found in the nuclease domain of a subfamily of bacteria toxins, including colicin E7, that are capable of cleaving DNA nonspecifically. This H-N-H motif has also been identified in a subfamily of homing endonucleases, which cleave DNA site specifically. To better understand the role of metal ions in the H-N-H motif during DNA hydrolysis, we crystallized the nuclease domain of colicin E7 (nuclease-ColE7) in complex with its inhibitor Im7 in two different crystal forms, and we resolved the structures of EDTA-treated, Zn(2+)-bound and Mn(2+)-bound complexes in the presence of phosphate ions at resolutions of 2.6 A to 2.0 A. This study offers the first determination of the structure of a metal-free and substrate-free enzyme in the H-N-H family. The H-N-H motif contains two antiparallel beta-strands linked to a C-terminal alpha-helix, with a divalent metal ion located in the center. Here we show that the metal-binding sites in the center of the H-N-H motif, for the EDTA-treated and Mg(2+)-soaked complex crystals, were occupied by water molecules, indicating that an alkaline earth metal ion does not reside in the same position as a transition metal ion in the H-N-H motif. However, a Zn(2+) or Mn(2+) ions were observed in the center of the H-N-H motif in cases of Zn(2+) or Mn(2+)-soaked crystals, as confirmed in anomalous difference maps. A phosphate ion was found to bridge between the divalent transition metal ion and His545. Based on these structures and structural comparisons with other nucleases, we suggest a functional role for the divalent transition metal ion in the H-N-H motif in stabilizing the phosphoanion in the transition state during hydrolysis.


Subject(s)
Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Cations, Divalent/metabolism , Colicins , Deoxyribonucleases/chemistry , Deoxyribonucleases/metabolism , Metals/metabolism , Phosphates/metabolism , Amino Acid Motifs , Amino Acid Sequence , Binding Sites , DNA/metabolism , Models, Molecular , Molecular Sequence Data , Protein Binding , Protein Structure, Tertiary , X-Ray Diffraction
20.
Biochemistry ; 41(27): 8759-66, 2002 Jul 09.
Article in English | MEDLINE | ID: mdl-12093295

ABSTRACT

The possible structural and catalytic functions of the nine tryptophan amino acid residues, including Trp(54), Trp(105), Trp(112), Trp(141), Trp(148), Trp(165), Trp(186), Trp(198), and Trp(203) in Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase (Fs beta-glucanase), were characterized using site-directed mutagenesis, initial rate kinetics, fluorescence spectrometry, and structural modeling analysis. Kinetic studies showed that a 5-7-fold increase in K(m) value for lichenan was observed for W141F, W141H, and W203R mutant Fs beta-glucanases, and approximately 72-, 56-, 30-, 29.5-, 4.9-, and 4.3-fold decreases in k(cat) relative to that for the wild-type enzyme were observed for the W54F, W54Y, W141H, W203R, W141F, and W148F mutants, respectively. In contrast, W186F and W203F, unlike the other 12 mutants, exhibited a 1.4- and 4.2-fold increase in k(cat), respectively. W165F and W203R were the only two mutants that exhibited a 4-7-fold higher activity relative to the wild-type enzyme after they were incubated at pH 3.0 for 1 h. Fluorescence spectrometry indicated that all of the mutations on the nine tryptophan amino acid residues retained a folding similar to that of the wild-type enzyme. Structural modeling and kinetic studies suggest that Trp(54), Trp(141), Trp(148), and Trp(203) play important roles in maintaining structural integrity in the substrate-binding cleft and the catalytic efficiency of the enzyme.


Subject(s)
Bacteria/enzymology , Glycoside Hydrolases/chemistry , Glycoside Hydrolases/metabolism , Tryptophan , Amino Acid Sequence , Amino Acid Substitution , Base Sequence , Catalysis , DNA Primers , Kinetics , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Peptide Fragments/chemistry , Polymerase Chain Reaction , Protein Structure, Secondary , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Sequence Alignment , Sequence Homology, Amino Acid , Spectrometry, Fluorescence , Thermodynamics
SELECTION OF CITATIONS
SEARCH DETAIL
...