Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Anal Toxicol ; 42(5): 337-345, 2018 Jun 01.
Article in English | MEDLINE | ID: mdl-29373719

ABSTRACT

The aim of the study was to develop a LC-MS-MS method able to detect and quantify a number of frequently prescribed antipsychotic and antidepressant drugs for toxicological purposes. Separation of compounds was performed on a C-18 RP column by Ultra High-Pressure Chromatography over a 11 min run. A modified single step QuEChERS protocol consisted essentially by the addition of acetonitrile, potassium carbonate and magnesium sulfate in 100 µL of sample, vortexing, centrifugation and evaporation has been selected. The method achieves satisfactory recoveries for 15 psychotropic drugs with a mean R% of 85% and provides efficient purification of the sample from endogenous interferences, simplicity and short sample handling times. The method was validated and provided satisfactory accuracy with recoveries ranging from 85 to 113% and precision with CV ranging from 1.2 to 13.2%. LODs were determined to be from 0.0003 to 0.017 µg/mL while LOQs were from 0.001 to 0.05 µg/mL for the 15 drugs. Matrix effect was below 20% and the analytes were stable in the matrix for 3 weeks. The method proved to be suitable for both analysis of clinical samples for Therapeutic Drug Monitoring and antemortem or postmortem whole blood samples of forensic cases. A number of samples with clinical and forensic interest were successfully analyzed demonstrating the effectiveness of QuEChERS in this field.


Subject(s)
Antidepressive Agents/blood , Drug Monitoring/methods , Forensic Toxicology/methods , Hypnotics and Sedatives/blood , Psychotropic Drugs/blood , Substance Abuse Detection/methods , Analytic Sample Preparation Methods , Antidepressive Agents/chemistry , Cadaver , Calibration , Chromatography, High Pressure Liquid , Chromatography, Reverse-Phase , Drug Stability , Greece , Humans , Hypnotics and Sedatives/chemistry , Limit of Detection , Psychotropic Drugs/chemistry , Reproducibility of Results , Spectrometry, Mass, Electrospray Ionization , Tandem Mass Spectrometry
SELECTION OF CITATIONS
SEARCH DETAIL
...