MicroRNA-143 targets DNA methyltransferases 3A in colorectal cancer.

BACKGROUND: MicroRNAs (miRNAs) are 19-25-nucleotides regulatory non-protein-coding RNA molecules that regulate the expressions of a wide variety of genes, including some involved in cancer development. In this study, we investigated the possible role of miR-143 in colorectal cancer (CRC). METHODS: Expression levels of human mature miRNAs were examined using real-time PCR-based expression arrays on paired colorectal carcinomas and adjacent non-cancerous colonic tissues. The downregulation of miR-143 was further evaluated in colon cancer cell lines and in paired CRC and adjacent non-cancerous colonic tissues by qRT-PCR. Potential targets of miR-143 were defined. The functional effect of miR-143 and its targets was investigated in human colon cancer cell lines to confirm miRNA-target association. RESULTS: Both real-time PCR-based expression arrays and qRT-PCR showed that miR-143 was frequently downregulated in 87.5% (35 of 40) of colorectal carcinoma tissues compared with their adjacent non-cancerous colonic tissues. Using in silico predictions, DNA methyltranferase 3A (DNMT3A) was defined as a potential target of miR-143. Restoration of the miR-143 expression in colon cell lines decreased tumour cell growth and soft-agar colony formation, and downregulated the DNMT3A expression in both mRNA and protein levels. DNMT3A was shown to be a direct target of miR-143 by luciferase reporter assay. Furthermore, the miR-143 expression was observed to be inversely correlated with DNMT3A mRNA and protein expression in CRC tissues. CONCLUSION: Our findings suggest that miR-143 regulates DNMT3A in CRC. These findings elucidated a tumour-suppressive role of miR-143 in the epigenetic aberration of CRC, providing a potential development of miRNA-based targeted approaches for CRC therapy.

Riboregulator H19 induction of MDR1-associated drug resistance in human hepatocellular carcinoma cells.

Acquisition of drug resistance is one of the main obstacles encountered in cancer chemotherapy. Overexpression of multi-drug resistance 1 (MDR1) gene and its protein product P-glycoprotein, accompanied with a decrease in doxorubicin accumulation level, was observed in doxorubicin-resistant R-HepG2 cells, a subline derived by selection of human hepatocellular carcinoma HepG2 cells with doxorubicin. In addition, Northern-blot analysis revealed an eight fold upregulation of the imprinted H19 mRNA in R-HepG2 cells. H19 knockdown by transfection with antisense H19 oligonucleotides suppressed the MDR1/P-glycoprotein expression, increased the cellular doxorubicin accumulation level and sensitized doxorubicin toxicity in both HepG2 parent cells and R-HepG2 cells. Results from methylation-specific polymerase chain reaction analysis indicated that the MDR1 gene promoter was hypomethylated in R-HepG2 cells. Antisense H19 oligonucleotides transfection induced a marked increase in the percentage of MDR1 promoter methylation and decrease in MDR1 expression in R-HepG2 cells. Thus, the H19 gene is believed to induce P-glycoprotein expression and MDR1-associated drug resistance at least in liver cancer cells through regulation of MDR1 promoter methylation.

The critical role of caspases activation in hypoxia/reoxygenation induced apoptosis.

Hypoxia/reoxygenation insult can be found in many tissues, including heart, brain, and tumor. It is believed that cell death may be resulted after cells were subjected to chronic hypoxia or reoxygenation after chronic hypoxia. The molecular mechanism for reoxygenation induced cell death is so far not clear and will require further study, in particular, to be distinguished from the pathways associated only with chronic hypoxia. In this study, the cell death mechanism in human squamous carcinoma A431 cells after hypoxia/reoxygenation insult is examined. It is demonstrated that although caspase-9 and -3 were activated during both hypoxia and reoxygenation, only those caspases activated during reoxygenation were responsible for reoxygenation induced apoptosis. Activation of caspase-9 and -3 during reoxygenation is believed to be triggered by the ROS formation at the time of reoxygenation. Addition of catalase during reoxygenation was found to attenuate reoxygenation induced apoptosis and caspase activation.

The role of Raf-1 in radiation resistance of human hepatocellular carcinoma Hep G2 cells.

Development of radiation resistance is one of the major reasons that cancer cells do not respond to radiotherapy and the mechanism for resistance is still not clear. Two sublines of human hepatocellular carcinoma Hep G2 cells were established from cells that survived two different irradiation regimes, 2 Gy for 10 days or 10 Gy for 2 days, respectively. Using MTT assay, the radiation conditioned cells were found to be more resistant to gamma-irradiation and have a greater extent of potentially lethal damage repair (PLDR) for radiation than the parent cells. By Western blot analysis, the radiation-conditioned cells were found to overexpress Raf-1 which is known to regulate the radiation resistance of cells. Inhibition of Raf-1 expression by antisense oligonucleotides increased the radiation sensitivity of the radiation-conditioned cells while inhibitors of Ras (L744,832), PI3K (LY294002) and p38 (SB203580) had no effect. Moreover, antisense Raf-1 oligonucleotides also decreased the radiation induced PLDR capacity of the radiation conditioned cells. It is therefore suggested that Raf-1 may induce radiation resistance through an increase in radiation induced PLDR capacity in Hep G2 cells.

Reactive oxygen species mediate doxorubicin induced p53-independent apoptosis.

Doxorubicin (DOX) is a common anticancer drug. The mechanisms of DOX induced apoptosis and the involvement of reactive oxygen species (ROS) in apoptotic signaling were investigated in p53-null human osteosarcoma Saos-2 cells. Accumulation of pre-G1 phase cells and induction of DNA laddering, which are the hallmarks of apoptosis, were detected in cells at 48 h upon DOX treatment. Furthermore, DOX increased the intracellular hydrogen peroxide and superoxide levels, followed by mitochondrial membrane depolarization, cytochrome c release, caspase-3 activation, prior to DNA laddering in Saos-2 cells. In addition, DOX treatment also upregulated Bax and downregulated Bcl-2 levels in the cells. The role of ROS in DOX induced cell death was confirmed by the suppression effect of catalase on DOX induced ROS formation, mitochondrial cytochrome c release, procaspase-3 cleavage, and apoptosis in Saos-2 cells. The catalase treatment however only suppressed DOX induced Bax upregulation but had no effect on Bcl-2 downregulation. Results from the present study suggested that ROS might act as the signal molecules for DOX induced cell death and the process is still functional even in the absence of p53.

Serum cholesterol and dietary fat of two populations of southern Chinese.

Children in Hong Kong (HK) are the second generation of Chinese migrants from Guangdong Province and are leading a more affluent lifestyle than those in Jiangmen (JM). The association between affluence and coronary risk was investigated by comparing the serum cholesterol and dietary fat intake of children in HK and JM. Fasting serum cholesterol was examined in 94 HK children and 99 JM children, all aged seven, using the same enzymatic method by the same observer. Duplicate meals were collected in two subsamples of 20 children, one each from HK and JM and analysed for their total fat intake and fatty acid profile, again by the same observer using gravimetric methodology and gas chromatography. The mean (SD) cholesterol of HK children was 4.59 (0.83) mmol/l, significantly higher than that of JM, 4.16 (0.61) mmol/l. The daily fat intake by the HK children was 48 g, 37% higher than that of JM at 35 g. PS ratio was 0.6in HK and 0.8 in JM. Cl8-2/C14-0, the cholesterol-lowering ratio, was mostly below 10 in HK, whereas that of JM was between 10 and 40 Therefore, in order to lower the total serum cholesterol of Hong Kong children, dietary intervention to lessen total fat, in particular milk and animal fat, while moderately increasing fat consumption from vegetable sources would seem to be appropriate.

Reappraisal of radiofrequency ablation of multiple accessory pathways.

Complete electrophysiologic study and radiofrequency ablation were performed in 145 consecutive patients with Wolff-Parkinson-White syndrome. Presence of multiple accessory atrioventricular pathways was documented in 20 patients (13.8%); 17 had two, two had three, and one had four accessory pathways. Location of accessory pathways was posteroseptal in 18, left free wall in 15, right free wall in nine, and right midseptal in two. Of the 44 pathways, 36 were found during baseline electrophysiologic study and eight were found after successful ablation of the initially attempted pathways. After delivery 20 +/- 23 pulses (per patient) of radiofrequency energy (37 +/- 6 W, 70 +/- 30 seconds), 43 accessory pathways were ablated successfully without complications. Duration of the procedure (4.5 +/- 1.7 vs 3.7 +/- 1.6 hours, p < 0.05) and radiation exposure time (53 +/- 30 vs 38 +/- 18 minutes, p < 0.05) were longer in patients with multiple pathways, whereas the success rate (95% vs 95%, p > 0.05) and incidence of recurrent conduction (11% vs 11%, p > 0.05) were similar in patients with single or multiple accessory pathways. These findings confirmed that multiple accessory pathways were common in patients with Wolff-Parkinson-White syndrome, and these pathways could be ablated successfully by radiofrequency energy with a success rate comparable to that of a single accessory pathway.

Recurrent conduction in accessory pathway and possible new arrhythmias after radiofrequency catheter ablation.

Radiofrequency catheter ablation was performed in 142 patients with 166 accessory pathways. One hundred thirty-six patients with 160 accessory pathways underwent successful ablation in the first ablation session. Serial follow-up electrophysiologic studies were performed immediately (30 minutes), early (5 to 7 days), and late (3 to 6 months) after successful ablation to determine the recurrent accessory pathway conduction and possible new arrhythmias. After a minimum follow-up period of 6 months (mean, 14 +/- 3 months), accessory pathway conduction recurred in 13 patients (9.6%), with recurrent tachycardia in three patients (2.2%). Five of the recurrent accessory pathways had decremental conduction properties. Incidence of recurrent accessory pathway conduction was similar in different accessory pathway locations (6.4% to 9.0%). Patients with concealed accessory pathways (12.2 vs 2.9%; p < 0.05), and patients without accessory pathway potentials in the ablation site (15.5% vs 2.2%; p < 0.05) had a higher recurrence rate. Patients without tachycardia in the late electrophysiologic study did not have recurrent tachycardia during follow-up. New arrhythmias, including atrial and ventricular arrhythmias, which were detected by 24-hour Holter monitoring, were apparent only on the first day after ablation. The findings indicate that the overall incidence of recurrent accessory pathway conduction was low and that possible new arrhythmias were rare in the late follow-up period.

Arrhythmogenicity of catheter ablation in supraventricular tachycardia.

To evaluate arrhythmogenicity in patients who receive a modified direct-current (DC) shock ablation (distal pair of electrodes connected in common as the cathode) or radiofrequency (RF) ablation of supraventricular tachycardia, a prospective study was performed with signal-averaged ECG, 24-hour Holter monitoring, electrophysiologic study (EPS) for ventricular tachycardia (VT), and treadmill exercise test. Sixty-nine consecutive patients with documented paroxysmal supraventricular tachycardia were included. Twenty-eight patients proved to have atrioventricular nodal reentrant tachycardia, and 41 patients had atrioventricular reciprocating tachycardia that involved accessory atrioventricular pathways. The first 34 patients received DC shock ablation and the other 35 patients received RF ablation. Signal-averaged ECG, Holter monitoring, and EPS for VT were performed before ablation, immediately after ablation, then 1 week, 2 weeks (Holter monitoring), 1 month (except EPS), and 3 months after ablation. Treadmill exercise testing was performed before ablation, and at 1 week and 3 months after ablation. The root mean square, low-amplitude signal and QRS duration of signal-averaged ECG disclosed no significant change after either DC or RF ablation up to 3 months. Late potential developed in only one patient in the DC shock group and it was considered to be innocuous because neither VT nor ventricular fibrillation was noted or induced. Increases in the number of ventricular premature contractions and in short-run VT were detected by Holter monitoring in the first week after either mode of ablation (p < 0.001 for the DC shock group; p < 0.05 for the RF group), which were greater (p < 0.05) and lasted longer in the DC shock group than in the RF group.(ABSTRACT TRUNCATED AT 250 WORDS)

Selective radiofrequency catheter ablation of fast and slow pathways in 100 patients with atrioventricular nodal reentrant tachycardia.

One hundred patients received selective radiofrequency ablation of retrograde fast pathway (32 patients, group I) or slow pathway (68 patients, group II) to treat drug-refractory atrioventricular nodal reentrant tachycardia. In group I, a mean of 6 +/- 3 radiofrequency pulses eliminated the retrograde fast pathway. Thirty patients were free of symptoms and were not receiving antiarrhythmic drugs; two patients had accidental atrioventricular block. One patient had recurrent tachycardia and received a repeated ablation (slow pathway ablation). In group II, a mean of 9 +/- 4 radiofrequency pulses eliminated the slow pathway in 68 patients. All patients were free of symptoms and were not receiving antiarrhythmic drugs. One patient had recurrent tachycardia and received a repeated ablation. Serial follow-up electrophysiologic studies (immediate [20 to 30 minutes], early [5 to 7 days], and late [3 to 6 months]) showed that selective ablation of retrograde fast pathway was associated with nonspecific injury on the antegrade fast pathway (increase of AH interval) without effects on the slow pathway. Selective ablation of slow pathway was associated with nonspecific injury on the retrograde fast pathway in 15 patients (22%), but the antegrade fast pathway conduction parameters did not change significantly. Thus retrograde and antegrade fast pathway may be anatomically similar or have different sensitivities to radiofrequency energy, and slow pathway may be anatomically distinct from fast pathway. We conclude that (1) selective radiofrequency ablation of retrograde fast or slow pathway could cure atrioventricular nodal reentrant tachycardia with a high success rate (98%) and a low recurrence rate (2%) during a follow-up period of 6 to 18 months, but fast pathway ablation was associated with accidental atrioventricular block (5%), and (2) serial follow-up electrophysiologic studies elucidated the possible mechanisms of cure in atrioventricular nodal reentrant tachycardia.

Radiofrequency ablation of left-sided accessory atrioventricular pathways in patients with unusual coronary sinus.

Four patients with left-sided accessory pathways (APs) and unusual coronary sinus (CS) received radiofrequency ablation. Unusual CS included occlusion of CS (patient 1), acute angulation of proximal CS (patients 2 and 3), and narrowing of CS orifice and proximal segment (patient 4). CS catheterization and AP mapping along the CS could not be performed in the four patients. Radiofrequency ablation by left ventricular retrograde technique for the manifest left posteroseptal AP (patient 1), concealed left posterior AP (patient 2), and transseptal left atrial technique for the manifest left posteroseptal AP (patient 3) and manifest left posterior AP (patient 4) were performed successfully without CS catheter guidance. This study suggests that radiofrequency ablation of left-sided AP with unusual CS is feasible by some special techniques.

Reversibility of left ventricular dysfunction after successful catheter ablation of supraventricular reentrant tachycardia.

Fourteen patients (mean age, 48 +/- 19 years) with left ventricular dysfunction in the absence of underlying organic heart disease underwent catheter ablation (nine with direct-current energy and five with radiofrequency energy) to treat drug-refractory, symptomatic supraventricular reentrant tachycardia (mean duration of tachycardia, 22 +/- 17 years). Clinical tachycardias were accessory pathway-mediated tachyarrhythmia (12 patients) and atrioventricular nodal reentrant tachycardia (two patients). Changes of ventricular function after successful ablation, as assessed by radionuclide ventriculography and echocardiography, showed a decrease in left ventricular end-systolic dimension (39 +/- 6 mm to 34 +/- 6 mm; 32 +/- 6 mm; p < 0.05) and in left ventricular end-diastolic dimension (55 +/- 5 mm to 52 +/- 3 mm; 51 +/- 3 mm; p < 0.05) in the early (2 to 3 months) and late (6 to 8 months) follow-up periods, increase of nuclear ejection fraction (38% +/- 8% to 46% +/- 7%; p < 0.05) and fractional shortening (28% +/- 7% to 36% +/- 8%; p < 0.05) in the late follow-up period. Increase of fractional shortening was mainly due to decrease in the end-systolic dimension. These findings suggest that prolonged attacks of uncontrolled supraventricular tachycardia may result in left ventricular dysfunction, which is reversible after successful catheter ablation of the arrhythmias.

Reappraisal of intermediate-term follow-up of radiofrequency ablation of accessory atrioventricular pathways for treatment of Wolff-Parkinson-White syndrome.

To evaluate the safety and efficacy of catheter mediated radiofrequency (RF) ablation in patients with Wolff-Parkinson-White syndrome, 125 patients with accessory pathway (AP) mediated tachyarrhythmias underwent RF ablation. Right-sided APs were ablated from the atrial aspect of the tricuspid annulus (all from the femoral vein approach) and the left-sided APs were ablated from the atrial or ventricular aspect of the mitral annulus. Immediately after ablation, 3 of 8 APs (38%) and 131 of 137 APs (95%) were ablated successfully with RF through a small-tip (2 mm) and a large-tip (4 mm) electrode catheter, respectively. Seven of the 11 APs where RF ablation failed had a later successful DC ablation. During follow-up (3 to 22 months), 11 of the 114 patients (10%) with successful ablation had return of accessory pathway conduction (2 had recurrence of tachycardia (2%)). Complications included transient myocardial injury (peak CK-MB 15 +/- 3 IU/l), transient proarrhythmic effects (more atrial and ventricular premature beats), accidental AV block (1 patient), cardiac tamponade (1 patient) and suspicion of aortic dissection (1 patient). In successful sessions, procedure and radiation exposure time were 3.8 +/- 0.2 h and 45 +/- 4 min, respectively. This study confirms that RF ablation with a large-tip electrode catheter is an effective and relatively safe nonsurgical method for treatment of Wolff-Parkinson-White syndrome.

Radiofrequency catheter ablation for treatment of Wolff-Parkinson-White syndrome--short- and long-term follow-up.

One hundred and twenty-five patients with accessory pathways mediated tachyarrhythmias underwent radiofrequency ablation. Right-sided accessory pathways were ablated from the atrial aspect of the tricuspid anulus (all from the femoral vein approach) and the left-sided accessory pathways were ablated from the atrial or ventricular aspect of the mitral anulus. Immediately after the procedures, 3 of 8 accessory pathways (38%) and 131 of 137 accessory pathways (95%) were ablated successfully with radiofrequency through a small-tip (2 mm) and a large-tip (4 mm) electrode catheter, respectively. Seven of the 11 accessory pathways that failed radiofrequency ablation had a later successful direct current ablation. During follow-up (3 to 22 months), serial electrophysiological study showed that 11 of the 114 patients (10%) with successful ablation had return of accessory pathway conduction (2 had recurrence of tachycardia, 2%). Complications included accidental AV block (1 patient), cardiac tamponade (1 patient) and possible aortic dissection (1 patient). Transient proarrhythmic effects (more atrial and ventricular premature beats) were seen during the first week and sustained ventricular tachyarrhythmias were not inducible. In a successful session, procedure and radiation exposure times (including the time for diagnostic procedures) were 3.8 +/- 0.2 h and 45 +/- 4 min, respectively. This study confirms that radiofrequency ablation with a large-tip electrode catheter is an effective and relatively safe nonsurgical method for treatment of Wolff-Parkinson-White syndrome, with a low complication and recurrence rate.

Follow-up of a modified catheter ablation of the atrioventricular junction in patients with atrial tachyarrhythmias.

In order to reduce cumulative energy, minimize barotrauma and infranodal injury, a modified catheter ablation technique was performed on seventeen patients. These patients had drug refractory atrial tachyarrhythmias (AT) consisting of thirteen with paroxysmal atrial fibrillation (PAf), three with sick sinus syndrome (SSS) with PAf, and one having SSS with rapid left atrial tachycardia. This technique, using the femoral approach, consisted of delivering a direct-current (DC) shock using a dual electrode configuration, to the ablation site adjacent the atrioventricular (AV) junction just before the disappearance of His deflection on the His bundle electrogram. After delivering 1-4 DV shock (mean 2.8) (cumulative energy 556 +/- 260 joules), 9 pts had first degree AV block (1st AVB) and 8 pts had complete AV block (CAVB). Only two pts had a right bundle branch block after ablation. During the clinical follow-up (15.4 +/- 2.7 months), sixteen pts were asymptomatic and were free of antiarrhythmic drugs. One pt was asymptomatic with quinidine which was ineffective before ablation. Pacemaker implantation was performed in 10 pts as a back-up for symptomatic SSS and CAVB. The results show this modified technique is relatively safe and effective.

Reappraisal of electrical cure of atrioventricular nodal reentrant tachycardia--lessons from a modified catheter ablation technique.

A modified catheter ablation technique was studied prospectively in 29 patients with atrioventricular (AV) nodal reentrant tachycardia. A His bundle electrode catheter was used for mapping and ablation. Cathodic electroshocks (100-250 J) were delivered from the distal two electrodes (connected in common) of the His bundle catheter to the site selected for ablation. The optimal ablation site recorded the earliest retrograde atrial depolarization, simultaneous or earlier than the QRS complex, with absence of a His bundle deflection during AV nodal reentrant tachycardia. One additional electrical shock was delivered if complete abolition of retrograde VA conduction persisted for more than 30 min and AV nodal reentrant tachycardia was not inducible during isoproterenol and/or atropine administration. With a cumulative energy of 323 +/- 27 J and a mean of 2.3 +/- 0.5 shocks interruption or impairment of retrograde nodal conduction was achieved. Antegrade conduction, although modified, was preserved in 27 patients, with persistence of complete AV block in 2 patients. Two of the 27 patients still need antiarrhythmic agents to control tachycardia, the other 25 patients were free of tachycardia within a mean follow-up period of 13 +/- 2 months (range 7 to 20 months). Twenty-three patients received late follow-up electrophysiological studies (3-6 months after the ablation procedures), and the AV nodal function curves were classified into 4 types. The majority of the patients (15/23) had loss of retrograde conduction. Among the 8 patients with prolongation of retrograde conduction, 4 patients still had antegrade dual AV nodal property but all without inducible tachycardia. In conclusion, preferential interruption or impairment of retrograde conduction was the major, but not the sole, mechanism of electrical cure of AV nodal reentrant tachycardia.

Catheter ablation of free wall accessory atrioventricular pathways in 89 patients with Wolff-Parkinson-White syndrome--comparison of direct current and radiofrequency ablation.

To evaluate and compare the safety and efficacy of catheter-mediated direct current (DC) or radiofrequency (RF) ablation in patients with free wall accessory atrioventricular pathways, 89 patients with free wall accessory atrioventricular pathway (AP)-mediated tachyarrhythmias underwent catheter ablation. Electrophysiological parameters were similar in the patients with DC (group I, 29 patients with 30 APs) or RF (group II, 60 patients with 64 APs) ablation. Immediately after ablation, it was seen that 27 of 30 APs (90%) had been ablated successfully with DC, but two of the 27 APs had early return of conduction and received a second ablation session; three of eight APs (38%) and 53 of 56 APs (95%) were ablated successfully with RF through a small-tip (2 mm) and a large-tip (4 mm) electrode catheter, respectively. Seven of the eight APs who had a failed RF ablation later had a successful DC ablation. During the follow-up (group I, 14 to 27; group II, 8 to 14 months), all successfully ablated patients had no recurrence of tachycardia. Complications in DC ablation included transient hypotension (two patients), and pulmonary air-trapping (two patients); in RF ablation the complications included cardiac tamponade (1 patient) and suspicious aortic dissection (1 patient); myocardial injury (reflected by peak CK-MB, 34 +/- 5 vs 15 +/- 4 IU.l-1) and pro-arrhythmic effects (new atrial and ventricular arrhythmias) were more severe in those who had DC ablation. Procedure and radiation exposure time were significantly longer in RF ablation (DC, 3.6 +/- 0.2 h, 34 +/- 4 min; RF 4.0 +/- 0.4 h, 46 +/- 10 min). This study confirms that RF ablation with a large-tip electrode catheter is an effective and relatively safe non-surgical method for treatment of free wall accessory atrioventricular pathway-mediated tachyarrhythmias.

Catheter ablation of accessory atrioventricular pathways in 114 symptomatic patients with Wolff-Parkinson-White syndrome--a comparative study of direct-current and radiofrequency ablation.

To evaluate and compare the safety and efficacy of catheter-mediated direct-current and radiofrequency ablation in patients with Wolff-Parkinson-White syndrome, 114 patients with accessory pathway-mediated tachyarrhythmias underwent catheter ablation. Electrophysiologic parameters were similar in patients undergoing direct-current (group 1, 52 patients with 53 accessory pathways) and radiofrequency (group 2, 62 patients with 75 accessory pathways) ablation. Immediately after ablation, 50 of 53 accessory pathways (94%) were ablated successfully with direct current, but 2 of the 50 accessory pathways had early return of conduction and required a second ablation; 72 of 75 accessory pathways (96%) were ablated successfully with radiofrequency current. In the three accessory pathways in which radiofrequency ablation was unsuccessful, a later direct-current ablation was successful. During follow-up (group 1, 14 to 27 months; group 2, 8 to 13 months), none of the patients with successful ablation had a recurrence of tachycardia. Complications in direct-current ablation included transient hypotension (two patients), accidental atrioventricular block (one patient), and pulmonary air trapping (two patients); complications in radiofrequency ablation included cardiac tamponade (one patient) and suspicious aortic dissection (one patient). Myocardial injury and proarrhythmic effects were more severe in direct-current ablation. The length of the procedure and the radiation exposure time were significantly shorter in direct-current (3.5 +/- 0.2 hours, 30 +/- 4 minutes) than in radiofrequency (4.1 +/- 0.4 hours, 46 +/- 9 minutes) ablation. Findings in this study confirm the impression that radiofrequency ablation is associated with fewer complications than direct-current ablation and radiofrequency ablation with a large-tipped electrode catheter is an effective and relatively safe nonsurgical method for treatment of Wolff-Parkinson-White syndrome.

Comparison of direct-current and radiofrequency ablation of free wall accessory atrioventricular pathways in the Wolff-Parkinson-White syndrome.

To evaluate and compare the safety and efficacy of catheter-mediated direct-current (DC) or radiofrequency (RF) ablation in patients with free wall accessory atrioventricular pathways, 95 patients with free wall accessory atrioventricular pathway-mediated tachyarrhythmias underwent catheter ablation. Immediately after ablation, 27 of 30 accessory pathways (90%) were ablated successfully with DC, but 2 of the 27 had early return of conduction and received a second ablation session; 3 of 8 (38%) and 57 of 62 (92%) accessory pathways were ablated successfully with RF through a small-tip (2 mm) and a large-tip (4 mm) electrode catheter, respectively. Complications in DC ablation included transient hypotension (2 patients) and pulmonary air-trapping (2 patients) and in RF ablation, cardiac tamponade (1 patient) and suspicious aortic dissection (1 patient); myocardial injury and proarrhythmic effects were more severe in DC ablation. Procedure and radiation exposure time were significantly longer in RF ablation (DC, 3.6 +/- 0.2 hours, 34 +/- 4 minutes; RF 4.2 +/- 0.5 hours, 50 +/- 10 minutes). This study confirms that RF ablation is associated with little morbidity and few complications, and RF ablation with a large-tip electrode catheter is an effective and relatively safe nonsurgical method for treatment of free wall accessory atrioventricular pathway-mediated tachyarrhythmias.