ABSTRACT
Seroma formation after axillary lymph node dissection for metastatic melanoma is a common problem. We present the use of free microvascular tissue transfer to treat a chronic postoperative seroma developed after axillary lymph node dissection for metastatic melanoma.
ABSTRACT
Hibernoma is considered as a rare soft tissue benign tumor originating from brown fat. Few cases have been described so far in the literature; hibernoma was first described by Merkel in 1906, but it was named by Gery in 1914. We present the case of 33-year-old woman who presented with a palpable lump over superior aspect of her thigh associated with pain. Magnetic resonance imaging examination reported a "dumbbell"-shaped soft tissue tumor involving the right side of the pelvis and adductor compartment passing through the obturator foramen into the adductor compartment infiltrating the hip joint. Clinical and imaging features initially suggested liposarcoma, but ultrasound-guided biopsy and final surgical resection yielded the diagnosis of hibernoma. This rare soft tissue tumor can imitate malignancy, but magnetic resonance imaging and possibly guided biopsy are essential for preoperative planning as surgical resection remains the best management pathway.
ABSTRACT
Breast cancer in men is rare and only about 390 men in the UK are diagnosed with breast cancer each year with an incidence rate in the UK of 1.5 cases per 100 000 men. In addition, the increased use of radiotherapy for management of breast cancer has led to a reported increase of radiation induced angiosarcomas (RIAS) with an incidence of 0.05-0.3%. Here we report a unique and extremely rare case of RIAS of breast in a male patient. To our knowledge this is the only case in the literature of a radiation induced angiosarcoma of the breast in a male.
Subject(s)
Dermatologic Surgical Procedures/adverse effects , Hyperemia/etiology , Hyperemia/surgery , Intraoperative Complications/surgery , Microsurgery/methods , Surgical Flaps/adverse effects , Aged , Anastomosis, Surgical/adverse effects , Humans , Hyperemia/pathology , Intraoperative Complications/etiology , Intraoperative Complications/pathology , Male , Melanoma/surgery , Skin Neoplasms/surgeryABSTRACT
OBJECTIVES: As kallikrein-related peptidase 12 (KLK12) has been implicated in the cancer progression and alternative splicing plays significant role in this disease, the aim of this study was to examine the expression profile and the clinical impact of the KLK12 splice variants in breast cancer. DESIGN AND METHODS: Total RNA was isolated and reverse transcripted from 141 tissues. Afterwards, quantitative real-time PCR were conducted, followed by the performance of the comparative CT (2-ΔΔCT) method for relative quantification, whilst their correlation with the clinicopathological features of breast malignancies were assessed by statistical analysis. RESULTS: Both KLK12sv1/2 and KLK12sv3 showed higher expression in non-cancerous than in cancerous samples. KLKsv1/2 (Pâ¯=â¯0.001) upregulated and KLK12sv3 (Pâ¯<â¯0.001) downregulated in the malignant compared to the benign tumors and their discriminative ability was verified by ROC curve analysis. Moreover, KLK12sv3 was associated with grade (Pâ¯=â¯0.012) and hormonal receptor status (Pâ¯=â¯0.001). Furthermore, Kaplan-Meier and Cox regression analyses showed that patients with positive KLK12sv1/2 and KLK12sv3 levels presented a significantly longer disease-free survival (Pâ¯=â¯0.014 and Pâ¯=â¯0.013, respectively) and overall survival (Pâ¯=â¯0.062 and Pâ¯=â¯0.004, respectively). CONCLUSIONS: Our results demonstrate the discriminative value of KLK12sv1/2 and KLK12sv3 between benign and malignant breast tumors as well as their potential favorable prognostic significance in breast adenocarcinoma.
Subject(s)
Adenocarcinoma , Alternative Splicing , Breast Neoplasms , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Neoplastic , Kallikreins/biosynthesis , Neoplasm Proteins/biosynthesis , Adenocarcinoma/classification , Adenocarcinoma/enzymology , Adenocarcinoma/genetics , Adenocarcinoma/mortality , Adult , Aged , Aged, 80 and over , Breast Neoplasms/classification , Breast Neoplasms/enzymology , Breast Neoplasms/genetics , Breast Neoplasms/mortality , Disease-Free Survival , Female , Humans , Kallikreins/genetics , Middle Aged , Neoplasm Proteins/genetics , Survival RateABSTRACT
Kallikrein-related peptidases (KLKs) are a group of 15 serine proteases, hormonally regulated, and localized on chromosome 19q13.4. Alternative splicing is a process that plays significant role in the development, physiology, and different diseases, like cancer. Kallikrein family numbers more than 82 alternative transcripts. Understanding the role that those gene transcripts play in various cancer types, could lead to the discovery of diagnostic markers or drug targets. The present study was designed to analyze the expression profile of the splice variants of kallikrein-related peptidase 12 (KLK12) in breast cancer patients and to evaluate their clinical significance. KLK12 splice variants (KLK12sv3 and KLK12sv1/KLK12sv2) were examined in 69 tissue samples of breast cancer using quantitative real-time PCR as well as semi-quantitative PCR. Relative quantitative expression of KLK12 was statistically associated to clinicopathological parameters. From the splice variants examined, statistical associations with clinicopathological parameters were obtained only from KLK12sv3 variant. KLK12sv3 is more frequently expressed in tumors of lower grade (p = 0.040), early patient TNM stage (p = 0.024), and smaller tumor size (p = 0.023). Positive KLK12sv3 expression is associated with longer patient disease-free survival (DFS) (p = 0.042) and higher progesterone receptor concentration (p = 0.008). KLK12sv1/KLK12sv2 expression is statistically associated with KLK12sv3 expression (p = 0.001). KLK12sv3 can be regarded as a marker of good prognosis in breast cancer.