ABSTRACT
BACKGROUND: Electrospun (ES) poly(É-caprolactone) (PCL) is widely used to provide critical mechanical support in tissue engineering and regenerative medicine applications. Therefore, there is a clear need for understanding the change in the mechanical response of the membranes as the material degrades in physiological conditions. STUDY DESIGN: ES membranes with fiber diameters from 1.6 to 6.7 µm were exposed to in vitro conditions at 37°C in Dulbecco's modified Eagle's medium (DMEM) or dry for up to 6 months. METHODS: During this period, the mechanical properties were assessed using cyclic mechanical loading, and material properties such as crystallinity and ester bond degradation were measured. RESULTS: No significant difference was found for any parameters between samples kept dry and in DMEM. The increase in crystallinity was linear with time, while the ester bond degradation showed an inverse logarithmic correlation with time. All samples showed an increase in modulus with exposure time for the first loading cycle. Modulus changes for the consecutive loading cycles showed a nonlinear relationship to the exposure time that depended on membrane type and maximum strain. In addition, the recovered elastic range showed an expected increase with the maximum strain reached. The mechanical response of ES membranes was compared to experimental tensile properties of the human annulus fibrosus tissue and an in silico model of the intervertebral disk. The modulus of the tested membranes was at the lower range of the values found in literature, while the elastically recoverable strain after preconditioning for all membrane types lies within the desired strain range for this application. CONCLUSION: The long-term assessment under application-specific conditions allowed to establish the mechanical competence of the electrospun PCL membranes. It can be concluded that with the use of appropriate fixation, the membranes can be used to create a seal on the damaged AF.
ABSTRACT
Back pain commonly arises from intervertebral disc (IVD) damage including annulus fibrosus (AF) defects and nucleus pulposus (NP) loss. Poor IVD healing motivates developing tissue engineering repair strategies. This study evaluated a composite injectable IVD biomaterial repair strategy using carboxymethylcellulose-methylcellulose (CMC-MC) and genipin-crosslinked fibrin (FibGen) that mimic NP and AF properties, respectively. Bovine ex vivo caudal IVDs were evaluated in cyclic compression-tension, torsion, and compression-to-failure tests to determine IVD biomechanical properties, height loss, and herniation risk following experimentally-induced severe herniation injury and discectomy (4 mm biopsy defect with 20% NP removed). FibGen with and without CMC-MC had failure strength similar to discectomy injury suggesting no increased risk compared to surgical procedures, yet no biomaterials improved axial or torsional biomechanical properties suggesting they were incapable of adequately restoring AF tension. FibGen had the largest failure strength and was further evaluated in additional discectomy injury models with varying AF defect types (2 mm biopsy, 4 mm cruciate, 4 mm biopsy) and NP removal volume (0%, 20%). All simulated discectomy defects significantly compromised failure strength and biomechanical properties. The 0% NP removal group had mean values of axial biomechanical properties closer to intact levels than defects with 20% NP removed but they were not statistically different and 0% NP removal also decreased failure strength. FibGen with and without CMC-MC failed at super-physiological stress levels above simulated discectomy suggesting repair with these tissue engineered biomaterials may perform better than discectomy alone, although restored biomechanical function may require additional healing with the potential application of these biomaterials as sealants and cell/drug delivery carriers.
