ABSTRACT
We report experimental results on the inactivation of encephalomyocarditis virus, M13 bacteriophage, and Salmonella typhimurium by a visible femtosecond laser. Our results suggest that inactivation of virus and bacterium by a visible femtosecond laser involves completely different mechanisms. Inactivation of viruses by a visible femtosecond laser involves the breaking of hydrogen∕hydrophobic bonds or the separation of the weak protein links in the protein shell of a viral particle. In contrast, inactivation of bacteria is related to the damage of their DNAs due to irradiation of a visible femtosecond laser. Possible mechanisms for the inactivation of viruses and bacteria are discussed.
Subject(s)
Bacteriophage M13/radiation effects , Encephalomyocarditis virus/radiation effects , Lasers, Solid-State/therapeutic use , Salmonella typhimurium/radiation effects , Animals , Cattle , Circular Dichroism , DNA, Viral/radiation effects , Microscopy, Atomic Force , Microscopy, Fluorescence, Multiphoton , Serum Albumin, Bovine/chemistry , Serum Albumin, Bovine/radiation effects , Spectrometry, Fluorescence , Spectrum Analysis, Raman , Virion/radiation effectsABSTRACT
Raman spectroscopy is used to study low-wave-number (
