ABSTRACT
The technique of immune blotting was used to analyze surface proteins obtained from cells M Tuberculosis exposed to partialmode of delipidization. At that, there were applied serums of patients with tuberculosis of lungs; HIV agents and patients with concomitant infection tuberculosis-AIDS and also HIV-negative patients without clinical signs of disease of lungs and with chronic diseases of lungs of other etiology The fractions oflow-molecular antigens with molecular mass 6.5-30 kilodaltons became diagnostically significant. To this fraction of antigens reacted serums of all patients with tuberculosis of lungs and serums of 91% of patients with concomitant tuberculosis-AIDS infection. The antigens of protein fractions with high (70-100 kilodaltons) and interim (30-69 kilodaltons) molecular mass became diagnostically insignificant.
Subject(s)
Antigen-Antibody Complex/blood , Antigens, Bacterial/isolation & purification , Immune Sera/chemistry , Mycobacterium tuberculosis/chemistry , Tuberculosis, Pulmonary/diagnosis , Adult , Antigens, Bacterial/immunology , Asthma/blood , Asthma/diagnosis , Asthma/immunology , Case-Control Studies , Coinfection , Diagnosis, Differential , Electrophoresis, Polyacrylamide Gel , Female , HIV Infections/blood , HIV Infections/diagnosis , HIV Infections/immunology , HIV Infections/virology , HIV-1/physiology , Humans , Male , Mycobacterium tuberculosis/immunology , Pneumonia, Bacterial/blood , Pneumonia, Bacterial/diagnosis , Pneumonia, Bacterial/immunology , Pulmonary Disease, Chronic Obstructive/blood , Pulmonary Disease, Chronic Obstructive/diagnosis , Pulmonary Disease, Chronic Obstructive/immunology , Tuberculosis, Pulmonary/blood , Tuberculosis, Pulmonary/immunology , Tuberculosis, Pulmonary/microbiologyABSTRACT
Lipid balance was studied in female patients with late rheumatoid arthritis, their healthy female relatives liable to autoimmune diseases, and healthy women without family history of autoimmune diseases. Previous studies showed that the relatives of patients with rheumatoid arthritis suffered from frequent stubborn common infections, which prompted us to analyze the relationship between lipid metabolism and the infectious syndrome parameters. Blood serum and cells were collected for analysis when females had no clinical symptoms of infections (in all groups) or laboratory signs of inflammatory process (in the relatives and controls). Proatherogenic shifts in serum lipid composition presumably associated with frequent lasting infections were detected in individuals liable to rheumatoid arthritis development. Elevated cholesterol content in mononuclear leukocytes in this group could lead, in turn, to dysfunctions of these cells and augment the defects of anti-infection defense. The parameters of lipid balance in patients with late rheumatoid arthritis were close to the age-specific norm.
Subject(s)
Arthritis, Rheumatoid/blood , Cholesterol/blood , Lipid Metabolism , Adult , Aged , Arthritis, Rheumatoid/etiology , Arthritis, Rheumatoid/immunology , Case-Control Studies , Cell Membrane/metabolism , Female , Filipin/blood , Humans , Infections/complications , Infections/immunology , Lymphocytes/metabolism , Middle Aged , Pyrenes/bloodABSTRACT
Radioisotope study of mononuclear phagocytes from patients with rheumatoid arthritis showed impaired ingestion of bacteria in the presence of pronounced digestive activity. Excessive accumulation methylumbelliferyl phosphate beta-glucuronide (product of hydrolysis catalyzed by glucuronidase released from cells) into the incubation medium was observed. This was probably related to the predominance of extracellular digestion.
Subject(s)
Arthritis, Rheumatoid/physiopathology , Phagocytosis , Arthritis, Rheumatoid/enzymology , Glucuronidase/metabolism , Humans , In Vitro Techniques , Lysosomes/enzymology , Phagocytes/enzymology , Phagocytes/physiologyABSTRACT
Experiments on guinea pigs showed that xymedon in a dose of 30 mg/kg stimulates nucleic acid synthesis in thymocytes and increases the blood serum nucleic acid content. Xymedon increased secretion of endogenous DNA by human lymphocytes stimulated with PNA in vitro. The systemic immunotropic mechanisms of pyrimidine derivatives are discussed.
