ABSTRACT
Life span depends on many factors, including the level of reactive oxygen species, like superoxide radical. Superoxide radical is produced from oxygen in the course of the oxidation of NADPH to NADP+. The process is catalyzed by NADPH oxidase. In this study, genotype and allele distributions of the C242T polymorphism in the CYBA gene, which encodes the alpha subunit ofNADPH oxidase (p22phox), were examined in the sample of long livers and in the population sample of the city of Tomsk. Statistically significantly higher frequency of T allele among female long livers (34.62%), compared to the females from Russian population (26.32%) was demonstrated (chi2 = 5.226; p = 0.022; OR = 1.48). Thus, the T allele is associated with a high life expectancy in females from the Russian population. No such association was observed for males from the same population.
Subject(s)
Longevity/genetics , NADPH Oxidases/genetics , Adult , Alleles , Female , Genotype , Humans , Male , Polymorphism, Single Nucleotide , Reactive Oxygen Species/metabolism , Risk Factors , Russia , Sex Characteristics , SuperoxidesABSTRACT
In this study we genotyped polymorphism in GPX1 Pro198Leu (C > T) rs 1050450 in four groups: patients with coronary artery disease, long-livers - above 90 years, early died peoples (before 55 years) from cardiovascular diseases and Russian population as control group. We have found significant higher allele T frequency in men with coronary artery disease -34.84% (Chi2 = 5.228, p = 0.022; OR = 1.46) and in early died men from cardiovascular diseases--38.16% (Chi2 = 6.461, p = 0.011; OR = 1.69) compared with control men--26.8%. Moreover, significantly higher genotype TT frequency has been shown in patients with coronary artery disease and myocardial infarction before age 50--19.44% in comparison with control group--7.28% (Chi2 = 9.55, p = 0.002). The TT frequency in long-livers (4.39%) was the lowest and significantly different from coronary artery disease group--12.79% (Chi2 = 8.07, p = 0.0045) and from coronary artery disease subgroup with myocardial infarction before 50--19.44% (Chi2 = 14.49, p = 0.0001). Thus our results indicate that allele T (Leu) of GPX1 Pro198Leu (C > T) polymorphism is unfavorable for successful ageing. It predisposes to coronary heart disease, earlier myocardial infarction (before age 50) and earlier death (before age 55).
Subject(s)
Coronary Artery Disease/genetics , Glutathione Peroxidase/genetics , Longevity/genetics , Myocardial Infarction/genetics , White People , Age Factors , Aged, 80 and over , Alleles , Case-Control Studies , Coronary Artery Disease/mortality , Female , Gene Frequency , Genetic Association Studies , Genetic Predisposition to Disease , Humans , Male , Middle Aged , Myocardial Infarction/mortality , Polymorphism, Single Nucleotide , Risk Factors , Russia , Glutathione Peroxidase GPX1ABSTRACT
The role of A2350G polymorphism in exon 17 of the ACE gene and A1166C - in 3'-UTR of the AGTR1 in the pathogenesis of left ventricular hypertrophy was studied in patients with essential hypertension (EH) and arterial hypertension combined with diabetes mellitus type 2 (AH + DM2). Patients with EH and AH + DM2 did not differ from the control sample of healthy individuals by allele or genotype frequencies. However, an association of both polymorphisms with LVH was detected in EH patients. The frequency of 1166C allele was higher in patients with LVH (33.6% vs 20.7% without LVH). A1166C polymorphism determined the magnitude of left ventricular mass index (LVMI) in EH patients as well (p = 0.007). 2350G allele frequency of the ACE gene was in 1.5, and GG genotype--in 3.5-fold higher in EH patients with LVH, as compared without LVH. LVMI was significantly higher in patients with GG genotype as compared with heterozygotes and AA homozygotes (p = 0.002). Thus the presence of 1166C allele of AGTR1 and 2350G allele of ACE can be considered as predisposing factors for LVH development in EH. In contrast, association of studied polymorphisms with presence or LVH degree was not detected in patients with arterial hypertension combined with DM2. This may indicate another structure of genetic component of predisposition to LVH in different causes.
Subject(s)
Hypertrophy, Left Ventricular/genetics , Peptidyl-Dipeptidase A/genetics , Polymorphism, Genetic , Receptor, Angiotensin, Type 2/genetics , 3' Untranslated Regions , Alleles , Diabetes Mellitus, Type 2/complications , Diabetes Mellitus, Type 2/genetics , Female , Gene Frequency , Humans , Hypertension/complications , Hypertension/genetics , Hypertrophy, Left Ventricular/complications , Male , Middle AgedABSTRACT
Results of the investigation of the genetic-demographic structure of Tomsk city are presented. It was shown that the Russians constitute about 90% population Tomsk. This population is characterized by intensive migration processes: only 30% of the investigated residents were born in Tomsk; maximal migration flow was observed from Western Siberia, Eastern Siberia, Kazakhstan and Ukraine. The population of Tomsk reproduces only 10% of its gene pool, while 40% of it is formed by the migrants from remote regions of the country. Dynamics of the portion of offsprings of heterolocal marriages in age groups was observed. Comparative analysis of genetical demographic structure of Tomsk's population and other previously studied urbanized populations of CIS was made.
