Specific Combinations of Erythrocyte Group Antigens in Blood Donors.

Blood groups are inherited biological characteristics that do not change throughout life only in healthy people. Blood group antigens determine the adaptation of a human, as a biological species, to the surrounding environment. Our goal was to study the combination and frequency characteristics of three group systems (ABO, RH, KELL) in blood donors. The blood of 743 donors (aged ≥ 18 years) has been investigated on erythrocyte blood group antigens (521 male and 222 female). The sample has been provided from diagnostic laboratory of "Health Centre Medina Ltd" of Batumi. Research was agreed with hematology department of the clinic. Blood typing of the sample has been carried out on the basis of immunogenetic laboratory of Batumi Shota Rustaveli State University. The express method with universal mono-clone antibodies was used in order to reveal blood group system antigens. ABO blood group are distributed the following order: O > A > B > AB. We allocated 16 phenotypic groups. O, Rh+, K- (38.62 ± 1.7%) are represented with the highest percentage among donors and a rather large number of the investigated donors (287 donors) is the carrier of this phenotype. A frequency distribution of the A, Rh+, K- phenotype group 10% (28.93 ± 1.6) was less. 215 donors carry A, Rh+, K- phenotype combination. O, Rh+, K+ and A, Rh+, K+ variations of the phenotype are represented with an equal percentage (3.23 ± 0.4%). 7.94 ± 0.9% donors are characterized by features of the O, Rh-, K- Erythrocyte group. A frequency distribution of B, Rh+, K- phenotype is slightly less - 6.86 ± 0.9%. A, Rh-, K combination of phenotype occurs with a frequency of 4.97 ± 0.7%, and AB, Rh+, K- occurs with a frequency of 2.28 ± 0.5%. I n the donor population there were only single cases of B, Rh-, K+ (0.13 ± 0.01); AB, Rh+, K+ (0.13 ± 0.01); AB, Rh-, K- (0.40 ± 0.2); B, Rh+, K+ (0.26 ± 0.1); O, Rh-, K+ (0.40 ± 0.2); A, Rh-, K+ (0.67 ± 0.2) combination of phenotype. The phenotype AB, Rh-, K+ was not identified. The existence of this database will increase the safety level of transfusion, widen donors database and allow the clinics to find quickly the rare combination of blood group for transfusion and decrease of the risk of post-transfusion complications.

SOME CLINICALLY IMPORTANT ERYTHROCYTE BLOOD GROUP ANTIGENS IN DONORS.

The distribution of erythrocyte blood group antigens was evaluated among 656 donors; samples were provided by the diagnostic laboratory "Medina" Ltd Health Centre of Batumi. Lab analysis of the sample was conducted by the immunogenetics laboratory at Batumi Shota Rustaveli State University. The frequency of the ABO allele system in donors was as follows: r (0.70), q (0.23), p (0.07). The distribution of Rhesus (Rh) factor in the donor population was as follows: Rh(-) was found among 16.3±1.43% of investigated donors; the Rh(+) phenotype was found in 83.7±1.43% of donors. Additionally, the CcDee phenotype frequency was 29.9±1.78%; CCD-ee was 17.2±1.47%; ccddee was 14.9±1.38%; and CcD-Ee was 13.9±1.34%; ccD-Ee phenotype was 11.1±1.22%; ccD-ee was 5.5±0.88%; same phenotype indicators -2.1±0.55 were observed for CcD-EE and ccD-EE; CCD-Ee was 1.4±0.45%, CCD-EE was 0.4±0.26%; and finally, the frequency of Ccddee phenotype amounts was 1.1±0.40%, ccddEe and CCddee phenotypes were both 0.2±0.17%. The analysis of the Kell system allele revealed a low frequency for the p allele at 0.05, whereas the frequency of the q allele was 0.95. This large epidemiologic analysis of donor blood provides valuable information for hematological and transfusion centers to inform the preparation of blood components for transfusion.

[Effects of metabolic triad (25% polarizing solution, mildronat, preductal MR) in acute myocardial infarction].

The aim of the work was to study the effect of metabolic triad with different mechanisms of acting--high-dose Glucose-Insulin-Potassium--25% polarizing solution (25% glucose, 50 IU soluble insulin and 4% 144 ml KCL-GIK), mildronat, preductal MR on the functional condition of heart during the acute myocardial infarction. 20 patients from the main group and 20 from the control one have been under the study. Patients with diabetes and heavy forms of heart failure (killip class>2) were not included in the study. Evaluation of the functional condition of heart was based on ECG and echocardiography data received before and after the treatment. It was determined that the frequency of the rhythm disorder decreases in the conditions of metabolic triad as well as during the thrombolitic reperfusion. Average period of time for normalization of S-T segment elevation made up 5.4+/-1.8 days and 7.3+/-1.2 days in case of the control group. The received data make it relevant to include the complex metabolic triad for preventive purpose during the complications followed after the acute myocardial infarction.

Continent urinary diversion (Gotsadze Pouch) after pelvic exenteration for gynaecological malignancies.

The problem of urinary diversion is of major importance in patients requiring pelvic exenteration for advanced gynaecological malignancies. Eight patients underwent pelvic exenteration (4-total and 4-anterior) at the Georgian Oncologic Centre, 7 of them for recurrent cervical carcinoma after combined treatment and 1--for primary advanced cervical carcinoma. The original surgical technique for construction of detubalarized ileal reservoir with continent umbilical stoma (Gotsadze Pouch) is described. Six patients underwent this type of urinary diversion with successful results. Urination is patient-controlled via self-catheterization every 5 to 6 hours. The results obtained by operation can be considered as optimal for their functional and rehabilitational properties.